The Effect Of Calf Serum On Cell Proliferation

1565 WordsOct 20, 20167 Pages
The purpose of this experiment was to observe how calf serum affected cell proliferation. It was hypothesized that various concentrations of Fetal Bovine Serum (FBS) would provide nutrients for non-transformed cells, and therefore affect their growth, but have no effect on the transformed cells. It was also hypothesized that the protein concentrations will increase for the non-transformed cells upon addition of the calf serum and FGF, but not affect the transformed cells since they are not dependent on growth factors. After analyzing the results, it can be concluded that increasing FBS concentrations does in fact increase non-transformed cell density. Transformed cells are unaffected by this concentration as is proven by the results since the cell densities are roughly the same at the differing concentrations of FBS (0.5%, 2%, and 10%). Furthermore, since the ERK1/2 factor is increased in the non-transformed cells, the cell density will also increase. Introduction: An increase in the population of cells is referred to as cell growth. This growth is monitored by regulation points after each stage of the cell cycle. Transformed cells differ from normal cells because they have an infinite lifespan. Transformed cells arise due to an alteration in the cell cycle. This alteration is passed on similarly to how traits are passed on in evolution. Since transformed cells are stronger than normal cells, they are ‘more fit’ for their environment. However, that does not necessarily mean
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