The Effect Of Catalase On Enzyme Activity Essay

1724 Words7 Pages
Effects On Enzyme Activity
This experiment tests the reaction rate of the enzyme catalase. Enzymes are proteins and they help to speed up chemical reactions. Catalase is an enzyme that helps to eliminate toxins such as hydrogen peroxide within an organism (Nuffield Foundation). When enzymatic reactions occur the reactant is known as a substrate. Within an organism enzymes are in a rather controlled environment where temperature and pH level stay in a tight range (Dominic Corsini). The hypothesis is that if concentration, temperature, or pH level increase in an enzyme then, the enzymatic reactions will speed up. The purpose of this experiment is to test the effect of temperature, enzyme concentration, substrate concentration, and pH level on an enzymatic reaction. In Experiment 1, Enzyme Concentration, the materials were 3 clean test tubes, a test tube rack, a ruler, a timer, a marker, catalase and hydrogen peroxide. Test tube 1 was marked at 1cm and 5cm. Test tube 2 was marked at 2cm and 6cm. Test tube 3 was marked at 3cm and 7cm. Test tube 1 was filled to the 1cm mark with catalase then hydrogen peroxide was added to the second mark at 5cm. The test tube was then swirled gently to mix. Test Tube 1 was then placed on the test tube rack and timed for 20 seconds for bubbling to develop. At the end of the time the ruler was used to measure the height of the bubbles from the surface of the liquid to the top of the bubbles and results were recorded. Test tube 2 was filled to
Get Access