The Effect Of Catalyse On Hydrogen Peroxide

• Fourthly, we kept the temperature at a constant 25°C using a water bath. At low temperatures, an increase in temperature causes an exponential increase in enzyme activity. This is because an increase in temperature provides more kinetic energy for the collisions of enzymes and substrates, so

The hypothesis is that catalase activity will increase exponentially with higher concentrations of hydrogen peroxide until all catalase active sites are filled, in which case the

Enzymes are biological catalysts, which means it decreases activation energy in reactions. The lower activation energy in a reaction, the faster the reaction rate. Many enzymes alter their shape when they bind to the activation site. This is called induced fit, meaning for the enzyme to work to its full potential it has to change shape to binding substrate. The location of enzyme’s activation site is on the surface of the enzyme, where the binding of substrates take place. Enzyme activity can be influenced by a variety of environmental factors. If the concentration of enzyme is low, and there is a great deal of substrate, then increasing enzyme concentration results in more molecules available to convert substrates to products. Thus, increasing enzyme concentration can increase reaction rate. If substrate concentrations are low, and many of the existing enzymes are idle because of a lack of substrate, then adding enzyme will have no effect on reaction rate. Enzyme concentration affects the enzyme activity, because the more enzyme concentration the faster the reaction rate, until it hits it’s limiting factor. When substrate concentration is increased, it also increases rate of reaction. Temperature plays an important

4.a) Describe the effect of low (pH 2) and high (pH 12) pH levels on catalase activity.

Students will be observing normal catalase reaction, the effect of temperature on enzyme activity, and the effect of pH on enzyme activity in this experiment. The enzymes will all around perform better when exposed in room temperature than when it is exposed to hot and cold temperatures. This is based on the fact that the higher the temperature, the better the enzymes will perform, but as the temperature reaches a certain high degree, the enzymes will start to denature, or lose their function.

This investigation will be carried out to investigate the rate of reaction of the enzyme catalase on the substrate hydrogen peroxide.

If the substrate concentration is increased then the rate of a catalase reaction will also increase until it reaches the optimal concentration or saturation point and will remain constant. This is because there will be more substrate molecules in a higher concentration therefore a higher frequency of collisions. This increases enzyme activity and more product will be formed. However at a certain concentration the enzymes will become saturated (all

These results show how temperature of extreme high, or low affects enzyme activity. The highest rate of enzyme activity occurred at 37 Cº. Anything that was hotter or cold than 37 Cº slowed the reaction rate. As I thought, 100 degrees would denature the enzyme, and that was the case. The data provided shows exactly what temperatures enzymes work best, and worst. The objective was achieved as we discovered the different reaction rates under different temperatures. The results are reliable, as we know enzymes do not work well when under extreme heat or denaturation occurs. What I learned in this experiment was that enzymes don’t work well under cold temperatures because they tend to move slower. My hypothesis did not quite match, because I thought they work best at lower temperatures.

Question: How does changing enzyme concentration or temperature affect the reaction time of enzyme activity?

The purpose of this experiment was to record catalase enzyme activity with different temperatures and substrate concentrations. It was hypothesized that, until all active sites were bound, as the substrate concentration increased, the reaction rate would increase. The first experiment consisted of five different substrate concentrations, 0.8%, 0.4%, 0.2%, 0.1%, and 0% H2O2. The second experiment was completed using 0.8% substrate concentration and four different temperatures of enzymes ranging from cold to boiled. It was hypothesized that as the temperature increased, the reaction rate would increase. This would occur until the enzyme was denatured. The results from the two experiments show that the more substrate concentration,

Between 36.7°C and 4° C there was a decrease in reaction rate. This is majorly due to the fact that less enzyme-substrate complexes are formed as the kinetic energy is decreased and both the catalase and hydrogen peroxide have less collisions with one another (“The Kinetic Molecular Theory”). A temperature decrease would have also caused the molecules to be more tightly packed together, thereby reducing the flexibility of catalase, making enzyme-substrate complexes less likely to occur.

In the experiment, it was proven that Tube 13 had the highest enzyme activity condition. The question being asked in this experiment is how does substrate concentration and pH affect enzyme function. It was hypothesized that there will be an optimal condition outside which function is lower. The group predicted that optimal conditions are pH is 7 and substrate concentration is 6 percent. In conclusion, this whole experiment justifies that enzymes are affected by the changes in pH. Where enzymes are most active is where you will find the most favorable pH value. According to the data, the substrate concentration with the highest enzyme activity is the 0 percent H2O2, which has a value of 0.0093. When compared to literature the 6 percent H2O2

The prediction of minimum activity occurring in 0° C and a maximum activity in 37° C was also supported. Through this lab it is easier to see when enzymes are at their weakest and their strongest. Within the body an enzyme is most efficient at a comfortable 37° C. For Catalase, its job in a perfect environment includes breaking down millions of hydrogen peroxide molecules every second (rcsb.org). As the temperature was increased, the reacting molecule H2O2 gained more kinetic energy, increasing the rate of bonds with the Catalase molecule and oxygen production.

If the temperature is too hot or too cold, then the reactivity and reaction rate of which the enzyme catalase breaks down hydrogen peroxide will decrease.

By observing the constructed graph, it is shown that the average rate of change initially increased as the temperature increased, but when temperatures increased past 50°C, the average rate of the reaction began to decrease until the 60°C point. therefore, the height of the reaction initially increased until the optimal point which was around 50°C and further decreased as the temperature was increased. This graph is very similar to what was predicted as the hypothesis. These results outline the effect of temperature on enzyme activity, and it is obvious that changes in temperature do have an effect on the enzyme catalase.