The Effect Of Changing The Polarity Of Mobile Phase On Retention Time

Introduction: The purpose of this lab is to see how fast an Alka-Seltzer tablet dissolves in water when the surface area increases. Problem Statement: How does surface area influence the Alka-Seltzer tablet on how fast it can dissolve in water? Hypothesis: If the surface area increases then the time it takes to dissolve will decrease. Investigation Diagram (ID)

6. The solubility of the solids were tested using a micro tray, by placing them in water and oil to observe their polarity,

I found that -DNA/LB was not nearly as moist as that -DNA/LB/AMP plate. The first one was dry and white.

Experimental Method: Polyethylene glycol 3350(PEG) was placed in water in order to measure the amount of time it took for the PEG to dissolve. The amount of both PEG and water were the same for each trial, the only changing factor was the temperature of the water. Timing started the moment PEG was placed into water, and the timer was stopped once PEG was dissolved.

From data table 3, Kf values range from 327 to 362, averaging out to be 345. [Fe3+] and [SCN-] initial concentrations were first calculated using Beer’s Law M1V=M2V2 dilution equation. Then [FeSCN2+] was calculated using the found concentration of [Fe3+] and [SCN-]. After that, [FeSCN2+]eq. was solved by (A eq.)/(A sd. ) [FeSCN2+]sd.

The eluting solvent passed down the column by the gravity and an equilibrium was established between the solute absorbed by the absorbent (silica gel in this experiment) and the eluting solving flowing down. Since the components in the sample had different polarity and they interacted with the stationary phase and the mobile phase differently, the components would be carried by the solvent to a different extent and a separation of the components could be achieved.

This will increase the rate the spots (compounds) move on the TLC plate. The reason for this is because if a more polar solvent is used, it will compete better with the polar stationary phase, resulting in all spots partitioning more in favor of the mobile phase, travelling faster, and having a higher

The entire process was repeated once more with 1.00-mL of 6.00-M phosphoric acid and 2.00mL of

An addition of 3 grams of anhydrous calcium chloride was put into the same flask and was stoppered to store for next lab

For Standard solution 1, we added 2 mL stock solution with 8 mL DI water

When an aqueous solution is added to a salt, NaCl, it causes it to be insoluble in water and therefore be recovered later on by having it crystalized. This is referred to as salting out, meaning that the polar organic compounds, which are soluble in water, are extracted into a nonpolar solvent. This is useful when it comes to drying of the aqueous extraction via the drying agent and in removing excess water from the organic solvent. Moreover, the drying agent used in this experiment was the Na2SO4. The drying agent allows for removal of water; however, if too much drying agent is used, it could lead to lose of the organic solvent by absorption.

When a solid and liquid combine together the solid will disappear, or dissolve. The hypothesis is, if the particles in the mixture are more tightly packed, then it will take less time for the mixture to separate, because particles in a suspension

The compounds are separated by collecting aliquots of the column effluent as a function of time.

Phosphorus is often associated with suspended solids, which have a significant relationship with turbidity. Strong relationship has been reported between turbidity and suspended solids, where turbidity has been used as a surrogate measure for total suspended solids (Acheampong et al. 2012; Packman et al. 1999). A consistent relationship has also been reported between total

2cm of a solution was tested and added 2 cm of 10% of potassium hydroxide solution and the test tube was shaked.