The Effect Of Concentration On The Ph Of A Buffer

In this lab, the purpose was to determine the stability of a substance after adding an acid or a base. The results claim that liver and buffer are the most resistance to change in pH. Looking at figure 3, buffer and liver both maintain a stable pH even with the addition of an acid or base. However, potato and water have less buffer in them since their pHs did change. In figure 3, the potato acid’s pH level decreased by two, and the potato base’s pH level increased by two. The level of pH of a water acid decreased by 4, while the water base’s pH increased by 5. These results all tie to the fact that buffer is a substance that maintains a stable pH; the presence of buffer in organisms help maintain homeostasis by binding or releasing hydrogen

A. How did the experiment in Part I demonstrate surface tension? Use your experiment observations when answering this question.

A buffer is a solution that resists changes in pH when H+, OH-, or H20 is added. By using standard lab equipment, a lab pro diagnostic tool, and acidic and basic solutions, the pH can be found. By recording the pH while adding a base or an acid gradually to a buffer solution you can find the capacity of each buffer to resist drastic changes in pH. The best buffers will keep a solution from becoming either too acidic or basic with the addition of a strong base or acid.

The normal blood pH is between 7.35 and 7.45. Allen’s blood was acidotic due to a decrease in lung expansion and an alteration in the perfusion to his spinal cord. He also has an alteration in spinal innervation to the respiratory muscles including the phrenic nerve that controls the diaphragm. This would further cause Allen to not be able to adequately take in enough oxygen and blow off enough CO2 to adequately have gas exchange, within the alveoli. Respiratory failure.

1. In the human blood, there is the bicarbonate buffer system. CO2 is released from cellular respiration and then taken up by red blood cells. Next, it is changed to carbonic acid which dissociates to form bicarbonate and H+ ions.

pH is a measurement of hydrogen ion concentration. It measures rather the solution is acid, base, or neutral.

We will observe the reaction of a buffer solution with added acids or bases. We will then evaluate buffering capacity in response to additions added from dilute acid and bases to the buffer.

In this experiment different pH levels ranging from 3 to 11 were used to test the effects on daphnia heart rate. The pH scale ranges from 0 to 14. A pH ranging from 0 to 6 is acidic, a pH of 7 is neutral, and a pH higher than 7 ranging from 8 to 14 is basic. PH revolves around hydrogen ions (H+). The reason pH levels can be acidic, basic, or neutral is because acids give hydrogen ions away while bases accept hydrogen ions. (Decelles, 2002).

How does the blood pH value change as Pco2 changes? The Pco2 binds with the water in the blood to form carbonic acid which then forms H+ and HCO3-. When the H+ increases it causes the blood to become more acidic and lowers the pH, and when the H+ is low the blood pH rises.

One of the basic concepts that new nurses need to learn is that homeostasis in the body is maintained by the acid base balance in the body. That concept is critical when looking at arterial blood gases. This can help guide the nurse to anticipate what the doctor will order and the education that she needs to give the patient and the family. This case study should help to illustrate the point.

The pH of a solution is the measure of the concentration of charged Hydrogen ions in that given solution. A solution with a pH lower than seven is considered to be acidic. A solution with a higher pH is a base. It is very important for organisms to maintain a stable pH. Biological molecules such as proteins function only at a certain pH level and any changes in pH can result in them not functioning properly. To maintain these constant pH levels, buffer solutions are used. A buffer solution can resist change to small additions of acids or base’s. A good buffer will have components that act like a base, and components that act like an acid.

For this experiment, titrations on a weak acid, acetic acid, and a buffer were performed. Acetic acid was titrated with NaOH in order to observe the half-equivalence point as well as the equivalence point. Then, the buffer and the buffered acetic acid solution prepared faced additional titration with NaOH and HCl to evaluate the differing buffering effects following the addition of a strong acid and strong base. Finally, the buffer’s buffering capacity was calculated. If the experiment were to be repeated, it would be interesting to observe the buffering effects following a titration between a weak base and a buffer instead with greater concentrations. The change in the concentration following the preparation of buffer with a weak base and its conjugate acid would pose for an interesting experiment to observe an increase in the buffering capacity.

Metabolic acidosis results from all conditions that decrease the pH of the body fluids below 7.35 and HCO3- levels are <22 mEq/L, with the exception of conditions resulting from altered function of the respiratory system. As hydrogen ions accumulate in the body fluids, buffers first resist a decline in pH. If the buffers cannot compensate for the increase in hydrogen ions, the respiratory center helps regulate the body fluid pH. The reduced pH stimulates the respiratory center, which causes hyperventilation. During hyperventilation, carbon dioxide is eliminated at a greater rate. The elimination of carbon dioxide also eliminates excess hydrogen ions and helps maintain the pH of the body fluids within a normal range. (Angus, 2006)

The hydrogen ion (H+) concentration is extremely important to living organisms. Even small changes in H+ ¬¬ion concentration can cause serious consequences to the structural and functional integrity of molecules. Consequently, it is important to regulate the pH within strict limits so that important biochemical processes of living systems can proceed normally.

Acidosis is when the pH of one’s blood is below the normal level of 7.35. Alkalosis is when the pH of one’s blood is above 7.45. These can be broken down into two categories respiratory and metabolic. Normal oxygen (CO2) levels are 35-45 mmHg in systemic arterial blood. Normal levels for HCO3− 22-26mEq/liter in systemic arterial blood.