The Effect Of Dl- 2 On Antioxidant Capacity And Its Related Gene Expression

1305 Words Sep 18th, 2016 6 Pages
Effects of DL- 2-hydroxy-4(methylthio)butanoic acid supplementation on antioxidant capacity and its related gene expression in lung and liver of broilers exposed to low temperature

J.P. Wang, G.L. Yang, K.Y. Zhang, X.M. Ding, S.P. Bai, Q.F. Zeng*

Institute of Animal Nutrition, Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education, Sichuan Agricultural University, Chengdu, Sichuan, China, 611130
*Corresponding author:Prof. Qiufeng, Zeng
Tel (Fax): 86-28-86290922; Email: zqf@sicau.edu.cn

Abstract
DL-2-hydroxy-4(methylthio)butanoic acid (DL-HMTBA) exhibits a higher antioxidant capability in vitro as compared to DL-methionine, but the mechanism is not known. A total of 400 8-d-old broilers were allotted to a 2 [Low (14-16℃) or control temperature] × 2 (0.17% or 0.51% DL-HMTBA) factorial arrangement to investigate effects of DL-HMTBA on the gene expression related to oxidative stress in low temperature. The hepatic glutathione (GSH), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities were decreased, while protein carbonyl and malodndialdehyde content in lung were increased in low temperature (P10-fold decrease in CβS protein levels. Therefore, it indicated that DL-HMTBA supplementation may increase MAT-1A and CβS gene expression, to enhance Met trans-sulfuration to GSH, then to increase antioxidant capacity of broilers exposed to low ambient temperature (39). Further work is needed to determine whether the…
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