The Effect Of Osmotic Pressure

If we placed our chicken egg into a higher percentage of sucrose solution for a set period of 60 minutes then the egg will gain weight. If the egg gained weight then the sucrose solution it was placed in had a low concentration that would make it hypotonic. If the egg loses weight then sucrose solution it was placed in had a high concentration that made it hypertonic. If there is no change in weight in the chicken egg when placed in the concentration the solution will happen to be isotonic.

In this lab experiment, half our group observed and measured osmosis using dialysis tubes that were represented as the semipermeable membrane. It is permeable to water and other small molecules but is impermeable to larger molecules such as the sucrose solution used in each of the four beakers and tubing. The other half of our group observed the tonicity of sheep blood to determine whether the blood was isotonic, hypotonic, or hypertonic. The 85 g/dL of NaCl solution was the ideal isotonic number in relation to the sheep blood cells as well as a reference to the other observations of the solutions.

Osmosis is a natural occurrence constantly happening within the cells of all living things. For osmosis to occur, water molecules must move across a semipermeable membrane from an area of low concentration to an are of high concentration. In order to understand osmosis, people must understand the different types of concentrations that can be present within solution. One of them is an Isotonic solution where the concentration of dissolved particles is equal to that of a cell’s. Another is a hypertonic solution where there is a higher concentration of dissolved particles then inside the cell. And lastly there is a hypotonic solution where there are less dissolved particles than inside the cell. As dissolved particles move to a region of lower concentration, water moves the opposite direction as a result of there being less water in the highly concentrated region. In this experiment, gummy bears were placed in salt water, sugar water, and tap water to find the measure of osmosis between the solution and gummy bear.

Osmosis is described in one of three ways when comparing more than one solution. The cell’s external and internal environment helps determine tonicity, which is defined as how the cell reacts to its environment. When the cell’s environment is equal in osmolarity to itself and there is no change, it is considered an isotonic solution. When the environment has a higher osmolarity, shrinkage occurs and it is considered a hypertonic solution. When the environment has a lower osmolarity, swellings occurs and it is considered hypotonic.

As the Concentration of the Sucrose Solution decreases, the more the potato’s mass increases. This is due to the solution being hypertonic. So, as the solute concentration gets lower, the potato’s water concentration will get higher, therefore more water particles from the solution will absorbed by the potato. Some changed very little in mass because the concentrations of the H2O molecules in the potato and outside the potato were equal. This equality in concentration is called Isotonic.

We know that water will move through a membrane from more free water to less free water. The higher the solute concentration is, the less free water there is. All of the sucrose solutions are being put in pure water, but the ones with the most sucrose will need the most water to diffuse inside and bring the free water levels to equilibrium, and the ones with less will need less water to diffuse.

The lab for this paper was conducted for the topic of osmosis, the movement of water from high to low concentration. Five artificial cells were created, each being filled with different concentrated solutions of sucrose. These artificial cells were placed in hypertonic, hypotonic, or isotonic solutions for a period of 90 min. Over time, the rate of osmosis was measured by calculating the weight of each artificial cell on given intervals (every 10 minutes). The resulting weights were recorded and the data was graphed. We then could draw conclusions on the lab.

In this experiment, we will investigate the effect of solute concentration on osmosis. A semi‐permeable membrane (dialysis tubing) and sucrose will create an osmotic environment similar to that of a cell. Using different concentrations of sucrose (which is unable to cross the membrane) will allow us to examine the net movement of water across the membrane.

The hypothesis states that if the solution is hypotonic the results will decrease, if the solution is hypertonic the results will increase and if the solution is isotonic the solution will vary and or remain constant. In order to test the predictions of the hypotonic, hypertonic, and isotonic hypothesis for the solution  made during the study,  four samples of sucrose were taken and placed into two different beakers each containing a different concentration. Then dialysis tubing A was placed into beaker 1 with B, C, and D placed into beaker 2 for 45 minutes and weighted at 15 minute intervals. My finding in the study was that each of the four samples changed from their initial weight and for the most part accurately proved the hypothesis.

Osmosis is the passive movement of water from an area of low solute concentration to an area of high solute concentration, normally across a membrane which prevents the movement of solvent. This is a process by which materials may move into, out of, or within cells. Osmosis doesn’t depend on energy provided by living organisms but is affected by the properties of the cell membrane. The rate of osmosis is dependent on such factors as temperature, pressure, molecular properties such as size and mass, and the concentration gradient. In osmosis, the relationship between a solute’s concentration outside of cell and inside of a cell is described in terms of the tonicity of the solution outside of the cell. A cell is in a hypotonic solution when the solute is more concentrated inside the cell and therefore water moves into the cell. In this solution the cell swells as water enters, this may continue until it ruptures or hemolyzes. In the reverse condition, the cell is in a hypertonic solution

The reasoning behind this experiment is the examine whether the rate of osmosis is changed due to a change in temperature. It was hypothesized that the rate of osmosis will increase as the temperature of the sucrose is increased. The rate of osmosis was tested by using the different jars full of different temperate water and testing how high the water rose on an osmometer over a span of 20 minutes. An osmometer is a tool used to measure rates of osmosis. The different temperatures tested on a sucrose solution were 5 degrees Celsius, 20 degrees Celsius, and 37 degrees Celsius. Rates of osmosis were higher in the hot water than in the cold water and control. The results showed that the rate of osmosis increased as the temperature increased, henceforth the hypothesis was supported. In conclusion, the experiment showed how changes in temperature affect the rate of osmosis.

The major objective of the experiment was to test the effect of the concentration gradient on the diffusion rate. It was hypothesized that the greater the stronger the concentration gradient, the faster the rate of diffusion would be. To test this, dialysis tubes were submerged in different concentration fructose solutions. We weighed the tubes at specific time intervals to measure the rate of diffusion of water in each different solution. The results illustrated that increased concentration gradient increases the rate of diffusion of water in the tubes. We concluded that as concentration of the

b. Place crushed ice in the beaker so the water level is just below the top of the

Osmosis is defined as the tendency of water to flow through a semipermeable membrane to the side with a lower solute concentration. Water potential can be explained by solutes in a solution. The more positive a number is more likely it will lose water. Therefore should water potential be negative the cell the less likely it will lose water. In using potatoes the effects of the molarity of sucrose on the turgidity of plant cells. According to Clemson University, the average molarity of a White potato is between .24 M and .31 M when submerged in a sorbitol solution. This experiment was conducted with the purpose of explaining the relationship found between the mass in plants when put into varying concentrations of sucrose solutions. Should the potatoes be placed in a solution that contains 0.2M or .4M of sucrose solution it will be hypotonic and gain mass or if placed in .6M< it will be hypertonic and lose mass instead. Controlled Variables in this lab were: Composition of plastic cups, Brand of Russet Potatoes, Brand of Sweet Potatoes and the Temperature of the room. For independent variable that caused the results recorded it was the different Sucrose concentrations (0.0M, 0.2M, 0.4M, 0.6M, 0.8M, 1M). The dependent variable was the percentage change from the initial weighs to the final. The cup with .4 molarity was the closest to an isotonic solution and was used as the control group for the lab. Water potential is the free energy per mole of water. It is

Four eggs that were previously soaked in vinegar were placed into four beakers with different levels of a glucose solution. The four beakers were filled with distilled water, 0.5M glucose, 1.5M glucose and 2.0M glucose. After the eggs were placed in the solutions, they were left for 60 minutes but weighed every 15 minutes to record whether there was an increase or a decrease in mass. The various masses were recorded as well as the percent change in mass. These were then recorded as line graphs. From this it was able to be determined if the egg had been in a hypotonic, hypertonic or isotonic solution, thus being able to determine if osmosis had occurred.