The Effect Of Ph, Enzyme Concentration And Temperature On The Catalytic Rate Of Enzymes Essay

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The Enzyme Properties Lab was performed to analyze the effects of pH, enzyme concentration, and temperature on the catalytic rate of enzymes. Many functions within the human body require/involve enzymes. It was found that enzymes have an optimal pH level, catalytic rate increases as the concentration of the enzyme increases, and an optimal temperature. The enzyme that was used throughout the experiment was ALP (alkaline phosphatase enzyme). ALP functions best at a neutral pH level, as it had the largest calculated enzyme activity of 2.05E-03. When pH went above or below the neutral value the enzyme activity tended to fall. ALP’s calculated enzyme activity tended to increase with an increase in enzyme concentration, as the calculated enzyme activity of the high enzyme concentration environment was 0.004607, which was larger than the other conditions. The last part of the experiment involved temperature and it was found that the optimal temperature for the enzyme was 32°C with a calculated enzyme activity of 0.000353. When temperature was below or above the optimal temperature the calculated enzyme activity dropped. Enzymes can begin to change shape and lose function when conditions are not appropriate, which was seen in the enzyme activity of ALP in varying environments. It can be concluded that enzymes play a major role in many reactions, but if conditions (pH, enzyme concentration, and temperature) are not appropriate enzyme activity tends to drop. The results supported the

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