The Effect Of Substrate Concentration On Activity Of Catalase

858 WordsOct 15, 20144 Pages
The purpose of this experiment was to analyze the effects of substrate concentration and temperature on activity of catalase. The activity was measured by observing the amount of time it took for 10 mL of oxygen to evolve as an outcome of the catalyzed breakdown of hydrogen peroxide. Increased temperature and substrate concentration will both effect the activity of a catalase enzyme. We used 4 different temperatures of an enzyme, as well as a variety of scientific materials. The results of this experiment confirmed the hypothesis that was tested. Introduction: A catalase is an enzyme that is typically found in all living organisms that are exposed to oxygen. A catalase catalyzes the decompositions of hydrogen peroxide to hydrogen and water. Catalase are categorized as tetramers because they are polymers consisting of four monomer units. The complex structure of the catalase tetramer as well as the hydrophobic nature of heme and its potential in generating reactive oxygen species indicate the need for tightly controlled and assisted assembly of catalase (Baureder). They are much different from the peroxidases, with considerable pleated sheet structure, and usually with a bound NADPH molecule (Dunford 12). Enzymes are important because they protect the cell from oxidative damage by reactive oxygen species. Catalase levels help to define the oxidative state of cells (Terlecky). Change was not predicted to occur if there was no catalase present. Although, the higher the
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