The Effect Of Temperature On Daphnia By Changing Ph Levels And Measuring The Hear Rate

1419 Words6 Pages
This experiment is being done to show the effect oh pH levels on daphnia by changing pH levels and measuring the hear rate.
Introduction
Daphnia magna are commonly used to test water toxicity, they are a vital part of fresh water ecosystems. They serve as a crucial role in food webs as they are a food source for smaller fish and invertebrates, and they also are a consumer of algae and bacteria (Elbert, 2005). They range from 2-5mm long these small crustaceans are also referred to as water fleas. Daphnia belong to the group called Daphniidae, which is a relative of freshwater shrimp. It is easy to see the internal organs of daphnia because of a transparent taco shell like carapace. A carapace is the hard outer part of a shell or
…show more content…
Also while measuring the heart rate observing these daphnia locomotion when exposed to these different pHs. The locomotion of the daphnia wasn’t measured it was just observed to see how these animals would react and maybe adapt to these pHs and maybe be able to survive.
Hypothesis
If the pH level is exceedingly acidic or basic then the heart rate of daphnia will drastically change compared to the neutral pH of 7. A pH of 3 and 4 will have the effect of the daphnia to have a high heart rate compared to a pH of 7. A base of 10 and 11 will have the effect of the daphnia to have a lower heart rate than the pH of 7. The locomotion of the daphnia will be much higher in the acidic pHs compared to the basic pHs. The comfortable pH for daphnia is between 7.2 and 8.5 (Clare, 2002).
Methods and materials
For making pH solutions For measuring heart rate
• Vinegar
• Spring water
• Baking soda
• Sodium hydroxide
• Clear plastic cups
• Computer
• Go link
• pH sensor
• Logger pro • Timer
• Pipettes
• pH solutions
• Concavity slide
• Black rubber rings
• Chap stick
• Light Microscope
• Daphnia
• Tally Clicker

When making the acidic pH solutions vinegar and spring water were used. For making the basic pH solutions sodium hydroxide, baking soda, and spring water is used to make the different pH solutions. For the higher basic pH solutions small amounts of sodium hydroxide was used. The pH sensor is put into the liquid and
    Get Access