The Effect Of Temperature On Daphnia By Changing Ph Levels And Measuring The Hear Rate

Introduction: Everyday household items can be seen as just “household items”. However, it is often not realized that we could actually determine the pH very easily. It could also be important to know the pH in everyday life. For example, it is important to keep track of human pH levels. Some people are curious to see the pH of their own body, which is a great use to keep acidic levels leveled. Another use in real life is determining the pH of pools. Whether it’s your own or a public pool, pH indicators come in hand to make sure the pool is sanitary and safe. pH indicators are indicators that contain halochromic synthetic compound which is added to a solution so that it can be

The following was the procedure used by the team that introduced chemicals into the environment of the Daphnia. First a zero reading was taken before any chemicals were introduced. The zero reading was an observation of the Daphnia’s heart rate before any substances were administered. All fluids were drawn off the slide using the corner of a Kimwipe. Then two drops of two percent alcohol solution were dropped onto the Daphnia. After a minute a heart rate reading was taken. The same procedure, including using the Kimwipe to draw off previous solution, was then used with four, six, eight, and ten percent solutions. A heart rate reading was taken after each solution was introduced.

Daphnia, also known as water fleas, are small crustaceans about 1mm-5mm long and are part of the freshwater zooplankton (Ebert 2005, Hutchinson 2005 & Clifford 1991). Daphnia can be found in most fresh water habitats such as freshwater springs, ponds and reservoirs and are the predominant food for planktivorous fish. Dapnia are ‘filter feeders’ meaning they feed on small particles suspended in the water which can include algae. It has been found that daphnia tend to migrate to the upper parts of the water at night and return to the lower parts of the water in the day to hide from predators (Ebert 2005) (Hutchinson 2005). Daphnia can reproduce through sexual reproduction and also asexual

To improve the results from the experiment buffer solutions that were not whole pHs could have been used e.g. pH 4.5, 5.5 etc. This would have provided more reliable results as a wider range of results would have been produced. Using pHs with decimals would also help to more accurately determine the optimum pH as the optimum may have been above or below the pH stated in the hypothesis; 8. In this experiment however the optimum is taken at 8 because the graph does not rise again.

When using different methods to measure pH levels there are some tools that can be useful. Some more than others but by putting into action the different methods it may determine which tools will work best and give the best results when testing the pH within a solution. The pH, which stands for the proportion of hydrogen ions in a solution, could be acidic (acidosis), neutral or basic (alkaline). The pH scale goes from numbers 1 through 14. A pH of 7 is neutral;

to the body of Daphnia. For example, an experiment may test the effects of toxic chemicals on Daphnia, meaning that Daphnia can act as water quality indicators (Ren et al. 2009). The lack of Daphnia in certain areas signifies the presence of a toxic chemical, if the habitat meets the living requirements of Daphnia (Villegas-Navarro, 2003). Therefore, Daphnia act as indicators of environmental health as well as chemical responders reacting to potentially harmful

The purpose of this experiment was to test the affect of caffeine on the heart rate of Daphnia by observing their behavior and heart rate under a microscope when exposed to different concentrations of caffeine. Caffeine is a stimulant drug used in many energy drinks and causes large amounts of stimulatory neurotransmitters to be released. Therefore, as caffeine is a stimulant drug it was hypothesized that the Daphnia heart rate would increase immensely. Daphnia is a group of microscopic, planktonic crustaceans that are arthropods that measure 1-5 millimeters in length. Daphnia live in multiple aquatic environments such as freshwater lakes, ponds and rivers. “The development of genomic infrastructure coupled with a wide range of phenotypic diversity make Daphnia a versatile model

Remember to use an efficient counting method and count the Daphnia’s heart rate for 15 seconds, multiply all your trials by four to get the beats per minute. Do no less than five trials to be accurate. Keep in mind that you need to test the Daphnia’s heart rate in its regular habitat before performing the

The Effects of Alcohol on Daphnia magna In this experiment I tested for the effect of alcohol on the Daphnia magna’s heart rate. I tested for alcohol and let water be the control to see the difference in heart rate. I hypothesized that alcohol will increase the heart rate of the D. magna, and my results positively supported my hypothesis. Introduction:

Investigating the Effect of Alcohol on Heartbeat of Daphnia Daphnia are the organisms that are involved in this experiment to find out what effect alcohol has on their heartbeat. It is easy to study the effects of alcohol on the heart of Daphnia as the organ can be easily seen through the transparent body of Daphnia. The number of heartbeats may be counted before submersion in alcohol and after submersion in alcohol to investigate the effect of alcohol. Daphnia belong to the Phylum Arthropoda and are Branchiopoda which belong to the class, Crustacea. Daphnia are invertebrates and also have an exoskeleton, jointed appendages, a dorsal heart and open blood system.

The experiment took place in a laboratory setting, and the first step was obtaining sixty individual Daphnia magna (that were neither adults nor tiny offspring) from a large tank in the lab. These individuals were equally divided into three groups; low density, medium density, and high density. The twenty Daphnia assigned to the low density group were split into four groups of five and pipetted into one of four tubes filled with 10mL of Chlamydomonas algae. The twenty Daphnia assigned to the medium density group were split into two groups of ten and placed into one of two tubes also filled up to 10mL with Chlamydomonas. The final twenty Daphnia were all placed into a single tube filled with 10mL of the algae. In order to avoid suffocation-related

must be above 8.5 and below 6.5. If the pH is too acidic, then the

In this experiment different pH levels ranging from 3 to 11 were used to test the effects on daphnia heart rate. The pH scale ranges from 0 to 14. A pH ranging from 0 to 6 is acidic, a pH of 7 is neutral, and a pH higher than 7 ranging from 8 to 14 is basic. PH revolves around hydrogen ions (H+). The reason pH levels can be acidic, basic, or neutral is because acids give hydrogen ions away while bases accept hydrogen ions. (Decelles, 2002).

The intention of this experiment is to determine the effects of pH on the rate of photosynthesis in living leaves. Photosynthesis is a process by which plants convert light energy captured from the sun into chemical energy which they use to perform various plant functions. During the photosynthesis process, light, carbon dioxide, and water react to produce products: sugar and oxygen. The equation for photosynthesis is:

What is the effect of varying pH solutions on the rate of photosynthesis on the Indian Lotus (Nelumbo nucifera) via The Floating Leaf Disc Assay?