The Effect of Substrates and Temperature on Lactose and its Shape, A list

460 WordsFeb 20, 20182 Pages
Title: The Effect of substrates and temperature on Lactase and its shape. Purpose: The purpose of this lab is to examine the specificity of the lactase enzyme to a specific substrate and how it can denature due to the rise in temperature. Background Information: Lactase is an enzyme that breaks down lactose. Lactose is the sugar found in milk. It is made up of two monosaccharides: glucose and galactose. Therefore it is a disaccharide that needs to break down in order to be digested. However some people are “Lactose Intolerant” or lack lactase in their bodies. This means that they cannot consume lactose-contained food or drinks or they need help digesting it. People who are lactose intolerant must be very selective to make sure that they do not eat food or drinks with lactose. There are pills though, which are purchased by lactose intolerant people. This pill helps the people break down the sugar lactose into its smaller components. Research Question: Will lactose in the milk react to lactose (will glucose be present) and will it react to the lactose in higher temperature then its optimum temperature? Independent Variable: lactose enzyme solution and denatured lactose enzyme solution Dependent Variable: glucose Control Variable: Milk Safety Rules: Do not put the lactase pill in your mouth. Unless your doctor prescribes you, you must not eat it. This could lead to an allergic reaction. Do not drink any of the materials needed for this lab. Do not

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