Introduction: On this Earth, every living species needs energy to live. Energy is a product of photosynthesis, which is the process that converts energy in sunlight to chemical forms of energy that can be used by biological systems2. Many organisms are not able to use the energy obtained from sunlight directly; however, plants are able to use this energy and convert it into chemical energy by converting CO2 (carbon dioxide) and H2O (water) to organic materials3. The energy source for photosynthesis comes from sunlight, which allows for the fuel that drives photosynthesis. This process can be affected by several variables; one of the most notable variables is the temperature of the plant's environment. Previous study has led me to …show more content…
As a control for the effect of temperature, one of the samples was kept at room temperature which is 25° C (Tube 3). Since the experiment we conducted on September 23, 2010 during Biology Lab 101 was conducted at room temperature, we had baseline data for what the rate of photosynthesis at this temperature would be. We concluded that this data could be used as a control to compare the rates of photosynthesis in both our experimental tubes. This tube was housed in a water bath set to 25° C. Contents of this tube included 1 mL of DPID, 1 mL of the buffer solution, 3 mL of water, and 3 drops of chloroplasts. Our first experimental tube (Tube 4) was kept in a water bath, at approximately 37° C. This tube was intended to measure the rate of photosynthesis at higher environmental temperatures. Contents of this tube included 1 mL of DPID, 1 mL of the buffer solution, 3 mL of water, and 3 drops of chloroplasts. All the Tubes were filled utilizing transfer pipettes to ensure the accuracy of volume being dispensed. Kimwipes were used to clean the tubes and wipes away any residual oils that could have been left from fingerprints. Distilled water was used to eliminate any potential bias that particles in the water could have had on the transmittance results. 0.1M phosphate solution was used for all tubes to control for the pH level. All tubes were covered with Parafilm and inverted
Have you ever really wondered how different variables can affect how plants go through photosynthesis? Well, in this experiment, the purpose was to see how various environmental conditions can affect the overall photosynthetic capacity of a specific plant. The factors, light, darkness, cold, and heat were applied to see how the different components would affect the photosynthesis on spinach plants. Each group was given a different factor to test. Out group was given the light factor. The hypothesis for this experiment is that when adding light as a factor, the light will affect the overall plant photosynthesis.
This lab was conducted to discover which level of light (measured in lux) would make Spinacia oleracea leaf disks float the fastest. Lux (lx) is latin for light, and is the unit of measurement for light in the International System of Units (Rouse 2011). Light plays a key role in the process of photosynthesis and in this we are essentially measuring the rate at which Spinacia oleracea leaf disks convert a bicarbonate solution into oxygen. The oxygen then would bubble up, allowing
Photosynthesis is the conversion of light energy to chemical energy into sugars. It is the process in plants that uses carbon dioxide, water, and sunlight from its surroundings and releases oxygen as a byproduct (6H2O+6CO2+light energy -> C6H12O6+6O2). Photosynthesis is required for plants because they are autotrophs, organisms that make their own food. Plants require a specific environment that is ideal to them to be able to carry out the process. Environmental conditions can either increase or decrease the rate of photosynthesis. Things like colors of light, pH, and temperature can all affect the rate of photosynthesis in plants.
The purpose of this lab is to observe the effect of white, green, and dark light on a photosynthetic plant using a volumeter and followed by the calculation of the net oxygen production using different wavelengths color of white and green light, and also the calculation of oxygen consumption under a dark environment, and finally the calculation of the gross oxygen production.
-Measuring the pH of a solution (such as in the lab we had) could also help determine the rate of photosynthesis. You would need a much more specific pH meter, but generally, if pH goes down, the level of CO2 is higher, meaning more cellular respiration. Higher pH means there’s less CO2, so more photosynthesis.
The rate of photosynthesis is affected by environmental factors like light intensity, light wavelength, and temperature. This experiment will test the
This lab will be driven by the research question, what is the effect of temperature (at 5oC, 15oC, 25oC, 35oC and 45oC) on the photosynthetic rate (measure of oxygen bubbles over a period of time) of Elodea (pondweed)?
Photosynthesis is a food making process for algae and plants. The photosynthesis process rate varies from different wavelengths and intensities of light. This lab will evaluate the optimal wavelengths and degrees of intensity during photosynthesis when chloroplast is exposed to light. The mixtures of DCPIP with water, PO4 buffer, and chloroplast will be prepared in a number of cuvettes. The cuvettes were tested individually at different wavelengths and intensities to find the optimal rate of photosynthesis by using a spectrophotometer, measuring the greatest change in absorbance. From this experiment, two data charts and four graphs were obtained. The hypothesis was set from graphs obtained in this lab, and the optimal reaction
The intention of this experiment is to determine the effects of pH on the rate of photosynthesis in living leaves. Photosynthesis is a process by which plants convert light energy captured from the sun into chemical energy which they use to perform various plant functions. During the photosynthesis process, light, carbon dioxide, and water react to produce products: sugar and oxygen. The equation for photosynthesis is:
photosynthesis happens in two stages: light reaction and carbon fixation also known as calvin cycle.light reaction TAKES PLACE IN THYLAKOID USE light energy to produces atp and nadph whereas, calvin cycle takes place instroma uses energy derived from light dependent reaction to make GA3P from CO2 ( Bio166 lab execise manual, 2015). the purpose of this experiment was to separate plant
For experiment one, data reveals clearly which light made the most impact on photosynthesis. There were no irregular results other than the decrease in the formation of bubbles throughout the second trial and a tiny increase in the third trial. This experiment, being the first to test, did not run smoothly as hoped. Problems such as the bubbles not forming were occurring at the beginning of this process due to the wrong type of plants being used, although this was resolved the following week in lab. This experiment requires no modification based on the clear results that were obtained. The results agreed with the prediction and in conclusion, the hypothesis has been supported and the intensity of the green light had greater increase and effectiveness to the plant than the natural or red light.
Experiment 1 Question: The main question that was addressed in the first experiment (Parts I and II) was which cell fraction had the most photosystem activity, which would be proportional to the number of chloroplasts present in the cell fraction. Photosystem activity is proportional to the number of chloroplasts since the photosystems are active in the thylakoid membranes of chloroplasts (Leicht and mcallister 2016). The amount of photosystem activity would be seen in the relative absorbance values for each cell fraction under the given light conditions. The absorbance would decrease as the DCIP electron acceptor (which is blue when oxidized) gets reduced (which is colorless). “The amount of color change is expected to be proportional to the number of functional photosystems, which in turn is proportional to the number of intact chloroplasts” (Leicht and mcallister 2016). We knew that chloroplasts were required for photosystem activity, but we
For lab 12, it is hypothesized that chlorophylls a and b are present in a plant leaf and contribute to the starch production in photosynthesis. Also, products of photosynthesis will be present in leaf tissue exposed to red and blue light wavelengths for several days, but a decreased presence in leaf tissue exposed to green and black light wavelengths. In lab 13, it is expected that since chlorophylls a and b are more polar and smaller molecules than the anthyocyanins and carotenoids, they will travel higher up the chromatography paper than the other pigments.
Photosynthesis is a huge concept to learn and understand in the field of biology. Plants have their own special way of using the ATP they produce. Photosynthesis is a process where plants harness the sunlight they receive and they produce carbohydrates, as well as oxygen for living things and other plants. Now the sunlight ultimately powers the process of
Photosynthesis is a biochemical process in which plant, algae, and some bacteria harness the energy of light to produce food. Nearly all living things depend on energy produced from photosynthesis for their nourishment, making it vital to life on Earth. It is also responsible for producing the oxygen that makes up a large portion of the Earth¡¦s atmosphere. Factors that affect photosynthesis are light intensity and wave length, carbon dioxide concentration, and temperature.