The Effect of Temperature on Photosynthesis

1085 WordsNov 10, 20105 Pages
Introduction: On this Earth, every living species needs energy to live. Energy is a product of photosynthesis, which is the process that converts energy in sunlight to chemical forms of energy that can be used by biological systems2. Many organisms are not able to use the energy obtained from sunlight directly; however, plants are able to use this energy and convert it into chemical energy by converting CO2 (carbon dioxide) and H2O (water) to organic materials3. The energy source for photosynthesis comes from sunlight, which allows for the fuel that drives photosynthesis. This process can be affected by several variables; one of the most notable variables is the temperature of the plant's environment. Previous study has led me to…show more content…
As a control for the effect of temperature, one of the samples was kept at room temperature which is 25° C (Tube 3). Since the experiment we conducted on September 23, 2010 during Biology Lab 101 was conducted at room temperature, we had baseline data for what the rate of photosynthesis at this temperature would be. We concluded that this data could be used as a control to compare the rates of photosynthesis in both our experimental tubes. This tube was housed in a water bath set to 25° C. Contents of this tube included 1 mL of DPID, 1 mL of the buffer solution, 3 mL of water, and 3 drops of chloroplasts. Our first experimental tube (Tube 4) was kept in a water bath, at approximately 37° C. This tube was intended to measure the rate of photosynthesis at higher environmental temperatures. Contents of this tube included 1 mL of DPID, 1 mL of the buffer solution, 3 mL of water, and 3 drops of chloroplasts. All the Tubes were filled utilizing transfer pipettes to ensure the accuracy of volume being dispensed. Kimwipes were used to clean the tubes and wipes away any residual oils that could have been left from fingerprints. Distilled water was used to eliminate any potential bias that particles in the water could have had on the transmittance results. 0.1M phosphate solution was used for all tubes to control for the pH level. All tubes were covered with Parafilm and inverted
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