The Effect of the Concentration Amylase on the Rate of Breakdown of Starch

1716 WordsJun 17, 20187 Pages
The Effect of the Concentration Amylase on the Rate of Breakdown of Starch Investigation: To find the effect of the concentration amylase on the rate of breakdown of starch. Prediction The lower the concentration of amylase, the slower the breakdown of starch will be. If you double the amount of amylase, the breakdown of starch will be quicker because there are twice as many active sites. I expect to see a graph like: [IMAGE] Rate of Breakdown [IMAGE] Concentration of amylase Research Enzymes are made of long protein molecules, which are folded up so they have an irregular globular shape. One part of the surface is the active site and fits exactly…show more content…
« Volume of enzyme must be kept the same. This can be decided during the pilot experiment. « I must mix the immediately after the enzyme has been added. « Temperature must be kept the same because if it is not kept the same in all of the test tubes, some of the enzymes will work faster in some tubes than in others. In order to keep the temperature the same, the tubes will be stood in cold water at room temperature, so that it will not fluctuate. Therefore, the experiment will work better because of the increase of kinetic energy and collisions. « The pH needs to be tested as amylase works best in alkaline conditions, because the pH effects the shape of the enzymes. The pH must be above 8.5 and below 6.5. If the pH is too acidic, then the enzyme will work slower in some test tubes than in others. In order to test the pH of the amylase a drop of the solution should be put on pH paper. This should be done both at the beginning of the experiment and at the end to see if the pH stayed the same throughout. « In order to keep it a fair test, I must choose a shade of brown that I will stop the stopwatch at. The reason for this is because if I leave the stopwatch on until the iodine turns dark brown and then on one of the other tubes until the iodine changes to light brown, the
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