The Effects Of 5 Fluorouracil, Penicillin G And Amphotericin On The Growth Of Pythium And Micrococcus Luteus

The antibiotics will have different zones of inhibition on different bacteria. Also, a range of concentrations of the nutrients in the bacteria will change the bacterial growth.

This experiment was designed to test and observe the transformation efficacy of the pUC18 and lux plasmids in making E. coli resistant to ampicillin. Both plasmids code for ampicillin resistance, however, the lux plasmid codes for a bioluminescence gene that is expressed if properly introduced into the bacteria’s genome. The E. coli cultures were mixed with a calcium chloride solution and then heat shocked, allowing the plasmids to enter the bacteria and assimilate into the bacterial DNA. The plasmids and the bacteria were then mixed in different test tubes and then evenly spread onto petri dishes using a bacterial spreader, heating the spreader between each sample to make sure there is no cross contamination. Each of the dishes was labeled and then incubated for a period of 24 hours. The results were rather odd because every single one of the samples grew. Several errors could have occurred here, cross contamination or possibly an error in preparation as every single sample in the class grew, meaning all samples of the bacteria transformed and became ampicillin resistant.

The purpose of this report is to analyse the growth of the bacteria known as Citrobacter Freundii as well as distinguishing what antibiotics effect its growth. This will be done so by answering the following question after completing its associated experiments. This question includes: what antibiotics are most effective in denaturing the bacteria? It has been predicted that chloramphenicol will be the most effective due to the fact that its medical uses are treating meningitis which is an infection caused by Citrobacter freundii. After conducting the experiments it was found that the chloramphenicol antibiotic was the most effective in denaturing the bacteria, although streptomycin was also affective. However, none of the other antibiotics were able to halt the growth of the bacteria.

The aim of this investigation was to find out which antiseptics were most effective at preventing the growth of bacteria.

The Effects of Antibiotics on Bacterial Growth Biology II 1996 Bacteria are the most common and ancient microorganisms on earth. Most bacteria are microscopic, measuring 1 micron in length. However, colonies of bacteria grown in a laboratory petri dish can be seen with the unaided eye. There are many divisions and classifications of bacteria that assist in identifying them.

The purpose of medicine is as contested of a notion as the term patient. While there are certain ends in which it is clear that medicine seeks to achieve, controversy often arises over how medicine chooses to achieve these certain goals. One such controversy is the treatment of patient-symptoms, more specifically, the role of medication in treating patients. The purpose of this essay is not to discuss the general issues of prescribing medication. Rather, the purpose is

These mutations, no matter what process that has led to their occurrence, block the action of antibiotics by interfering with their mechanism of action (1). Currently, antibiotics attack bacteria through one of two mechanisms. In both mechanisms the antibiotic enters the microbe and interferes with production of the components needed to form new bacterial cells. Some antibiotics act on the cell membrane, causing increased permeability and leakage of cell contents. Other antibiotics interfere with protein synthesis in cells. They block one or more of the steps involved in the transformation of nucleic acids into proteins.

In a study by Jun Lin et al., it was stated that bacitracin is an antibiotic that has a growth-promoting ability. This means that it would have no affect on inhibiting, or stopping the growth of bacteria, in particular, E. coli. On the other hand, a study done by Sukayna M. Fadlallah et al. proved that gentamicin proved a promising ground for treatment of E. Coli. Different molecular strains of E. coli were subject to different regimens of antibiotics, including gentamicin. The results

In order for amphotericin B to work, there must be sterols present. This is because it is susceptible to sterols. A bacterial membrane does not contain sterols, which means it can’t be used as an agent against bacterial infection. The antibiotic amphotericin B is an antifungal drug because Fungi have sterol in its membrane.

Tyrothricin, one of the first antibiotics discovered after penicillin, treated many wounds and helped fight diseases in the body (“Antibiotic” 1). Scientists tested the efficiency of Tyrothricin on open wounds. After twelve days, the Tyrothricin that had been administered to one of the wounds had miraculous results in comparison to the injury without the ointment (Williams 1). Tyrothricin has benefitted many people with flesh wounds particularly soldiers at war. Their accessibility to the drug had increased their bodies productivity in healing the laceration. Moreover, Actinomycete is another example of Penicillin blooming the beginning of all antibiotics. Actinomycete, when tested its capability of eliminating marine alga Phaeocystis globosa, proved to be highly efficient in that after 7 days, marine alga Phaeocystis globosa decreased its toxicity by 60% (Zhang 1). Actinomycetes contribution to society includes drugs like Bestain. When combined with Aminopeptidase, Bestain resists and reduces the size of liver cancer stem cells and also xenograft tumors (Sun 1). Derived from Penicillin, Actinomycetes is the benefactor and savior to the once ravenous diseases that once spread among people by producing many different antibiotics. Eventually, antibiotics created by cephalosporins commenced into society with drugs such as Cefixime, Cephalexin, and Cefuroxime which suppress infections like skin,

Chemotherapy is the use of drugs to prevent, control or remove the infection or disease. Antibiotics are substances derived from a microorganism or produced synthetically or semi-synthetically, that kills or limits the growth of a living organism. Whereas antibacterial agents used to kill bacteria or, suppress their growth or their ability to reproduce. According to the mechanism of action antimicrobial drugs are work in a several ways: inhibition of cell wall synthesis, inhibition of protein synthesis, inhibition of nucleic acid synthesis, inhibition of metabolic pathway, disruption of cell wall permeability and inhibition of viral enzymes. Also, antibiotic drugs further classified as bacteriostatic or bacteriocidal. Antimicrobial drugs either

Antimicrobial chemotherapy is a highly respected medical science which has shaped modern humanity in many ways including the furthering the understanding of medicine. A wide variety of antimicrobial substance have been discovered, designed and synthesized. Modern medicine is highly dependent on chemotherapeutic agents that are used to treat diseases. Antimicrobials are combined of chemotherapeutic agents and antibiotics. It can be defined as any substance of natural, synthetic, or semisynthetic origin which at low concentrations kill or inhibits growth of microorganisms and causes little or no damage. Antimicrobial Chemotherapy is a chemical compound that selectively inhibits or kills bacteria without suggestively harming the

The main objective of this experiment is to investigate the effect of different types of antibiotics on bacteria Bacillus subtilis and Escherichia coli. Some of the main methods used in this experiment

Tetracycline is a common antibiotic that is used to treat many patients that have infections such as urinary and respiratory infections. The objective of this research was to analyze the effects of Tetracycline on E.coli, in order to determine if it would have a dose response to the sensitive strain DH5Alpha. For the experiment, we had to prepare a bacterial Serial Dilution based on our MIC range of 1 µg/ml to 128 µg/ml. First, we prepared a bacterial serial dilution and placed the bacteria on LB agar, and incubated it at 37 degrees Celsius until the next lab period. During the incubation period, we began to calculate our tetracycline serial dilution. Then began preparing the samples by adding them to bacterial plates. We then incubated the bacterial plates that have tetracycline. After a week of incubation, we interpreted our results from the antibiotic plating. The Results showed that any concentration lower than 0.15 µg/ml will show a growth in bacteria compared to a concentration higher than 0.15 µg/ml that showed no growth of bacteria. Therefore, the minimum inhibitory concentration of Tetracycline that would inhibit growth of E. Coli is 0.15 µg/ml.

Antibiotics are chemical compounds that selectively interfere with the growth of microorganisms while causing little or no damage to the animal or human host. The purpose of this lab was to determine the effectiveness antibiotics against any given bacterium by means of examining the zone of inhibition, the minimum inhibitory concentration, and the minimum bactericidal concentration.