The Effects Of A Putative Anti Cancer Drug On Hela Cells
3121 WordsOct 31, 201413 Pages
According to the Office of National Statistics, in 2013, 29% of deaths in the UK were cancer related, making it the leading broad cause of death in the population. In the United States, 1 in 4 deaths are cancer related, and it is estimated that in a lifetime, there is 44% chance of contracting an invasive cancer if you are male, and a 38% chance if you are female (Siegel et al. 2014). So, although our knowledge and education into different cancer mutagens such as UV radiation, chemical carcinogens and viruses (Alberts et al. 2002), as well as cancer physiology and treatments may have improved, leading to a decrease in cancer related mortalities, there are still huge advances to be had in the field (CRUK 2013).…show more content…
In another review paper in Nature, Masters (2000) described how cancer cell lines are usually from fast growing, undifferentiated cancers that are easily transported and proliferated. HeLa cells may be differentiated cervical carcinoma cells but they attain their ability to proliferate uncontrollably from the protein HPV-18, which, combined with their ability to divide indefinitely, due to their chromosomes not shortening on replication, mean that they are the perfect cell line to grow in vitro (Watson 2010). Therefore, the HeLa model was used worldwide in research for not only cancers and development of different chemotherapeutic drugs such as Cannabinoids (Guzman 2003), but also in the research of genomics and vaccines including that for polio. It should also be noted, that the conditions in a laboratory lend themselves to a very minimal level of genetic drift and combined with control measures that assure that new cells are used every few weeks, the HeLa cell line is actually very similar to that of the original group of cells (Masters 2000). These characteristics mean that HeLa cells are an ideal cell line to use in this experiment.
In this experiment, analysis of cell viability will be achieved through an MTT assay. The salt 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) is often used in cancer drug screening (Vistica et al. 1991) as it is