The Effects Of Microbial Cell Culture On Human Development Essay

The purpose of this lab was to identify an unknown bacteria culture using differential tests. The identification of the unknown culture was accomplished by identifying the bacteria based on its specific metabolic characteristics and morphology. It is suggested that culture 11 is a sample of Enterobacter aerogenes.

A mixed culture of two unknown bacteria was provided by the instructor. The methods used for

For many years the identification of microorganisms has been important in the world of medicine. It is essential or correct disease diagnosis in patients and for proper treatment. Knowing the correct identity and characteristics of microorganism is crucial when disease outbreaks occur in populations, also knowing how humans can benefit from microorganisms is important; many can be used in making certain foods or antibiotics.

The purpose of this lab was to identify two unknown bacteria cultures using various differential tests. The identification of these unknown cultures was accomplished by separating and differentiating possible

Purpose: The purpose of this lab is to help you become a little familiar with some of the tests that can be typically performed in a clinical or research lab facility. These tests may help in determining a particular pathogen’s growth needs.

There are many reasons for knowing the identity of microorganisms. The reasons range from knowing the causative agent of a disease in a patient, so as to know how it can be treated, to knowing the correct microorganism to be used for making certain foods or antibiotics. This study was done by applying all of the methods that have been learned so far in the microbiology laboratory class for the identification of unknown bacteria. The identification process can be completed with a series of deferential stains and biochemical tests. Creating a dichotomous key helps to limit the amount of biochemical tests done on an unknown organism and by observation

In this experiment, an unknown bacterium was given to each individual student. The main purpose of this lab was to identify the given unknown bacteria going through a series of biochemical tests as one of the gram negative bacteria among six different Gram negative bacteria Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa and Salmonella typhimurium. At the very beginning, streaking method; T-streak technique was used to isolate the pure colonies. For the morphological identification of unknown bacteria, Gram Stain Method was done. Biochemical tests that were conducted for the experiment

The purpose of the Unknown Project is to help microbiology students learn to identify an unknown bacterium in a mixed culture by performing different types of tests in a lab. The student must be familiar with the tests and should be able to analyze and interpret the results. Based on the results attained, an organism may be proposed. But what is a mixed culture? A mixed culture contains either two or more different types of identified microorganisms in a container, like a test tube for example. The culture may carry either organisms from the same bacterial family or it may contain a mixture of fungi and bacteria or other combinations. Being able to identify different types of organisms is essential for us to know if by any case, risks are presented. These cultures are performed mainly in hospitals. They are obtained from different parts of a patient’s body, depending on the suspected infection. Registered Nurses can perform certain cultures like blood, urine, sputum, etc. and send them to the lab. A mixed culture has been helpful in previous medical research by helping determine whether a patient has a bacterial infection or not. For example, “Sputum samples often give mixed cultures. Since the patient coughs up the sputum from the lungs, the sample can become contaminated by normal mouth bacteria, resulting in multiple types of bacteria in the culture.” (http://www.ask.com/science/mixed-culture-microbiology-af200d033ce00db0) Many tests may be performed on this mixed

When reflecting back to experiment 3, Aseptic Technique and Culturing Microbes, I realized the large amount of microorganisms that can be found in everyday life. Many different types are found with in the human body. Theses experiments focused on two types of bacteria. First was Staphylococcus epidermidis, found on the skin, and second was Lactobacillus acidophilus, found in the gastrointestinal tract. Both have similar needs for growth when it comes to temperature, however, different growth environments are used.

A highly conserved gene will be used to identify a prokaryotic species isolated from the body. Fundamental lab techniques will be also explored and utilized, such as amplifying using PCR, cloning, and transforming the gene into a host cell. DNA electrophoresis and specific substrate plating will serve as analysis check points. The final product will be sequenced and compared to similar species to observe phylogenetic relationships.

Title Selective and Differential Media, Replica Plating, Rapid Microbiological Systems Andrisha Smith Microbiology 275 Dr.McCann October 20, 2017 Introduction Microbiological media is used to grow microorganisms, select or identify a particular type of microorganism based on aspects of their biochemistry. The two types of medias used in tis lab were selective and differential media. Selective medias are designed to grow some microorganisms and inhibit the growth of others.

This lab and its procedures are all about finding out the unknown identification of a given bacteria. The lab consists of specific techniques, tests, chemicals, and vocabulary that are necessary for the finding of the bacterial identity. A bacterium is randomly assigned and it is a group effort to find the bacteria name through many of its specialties and characteristics. An example of classifying it would be to determine whether the bacteria is catalase negative or positive, or if the species is gram negative or positive. This lab is of huge significance because of its medical microbiology connections. Scientists Gurtler and Stanisich explained the connections more eloquently. They stated, in their medical article, that, “Medical microbiology

Microbiology is a subject dealing with microbes and related concepts. Microbiology has come a long way since discovery of microbes and is presently of great help to mankind. It is used in health care, food production, diagnosis, production of alcohol, maintenance of sterility and cleanliness etc.

2. Introduction: Each student was given unknown bacteria and was instructed to perform a variety of experimental tests that would help to identify their bacteria. During the process of identification, the unknown bacteria was added to many different testing medias using aseptic technique. They are as follows: lactose fermentation on eosin methylene blue (EMB), TSI (Triple Sugar Iron agar), Phenol red sucrose, the SIM test, H2S by SIM, IMViC (indole, motility, voges-proskauer, and citrate), Urease (urea broth), PDase (Phenylalanine Deaminase), Lysine Decarboxylase, and Ornithine Decarboxylase. Colonial morphology on EMB was used to

Each mixed culture that was tested had one gram positive and one gram negative bacterial species. The possible species of bacteria that could have been isolated from the mixtures included the following: Bacillus subtilis, Corynebacterium glutamicum, Escherichia coli, Staphylococcus aureus, Enterococcus faecalis, Enterobacter aerogenes, Salmonella enterica, and Pseudomonas aeruginosa. The identities of the unknown species were determined through comparing the experimental data against data acquired from earlier experimentation.