The Effects Of Mixed Bacterial Culture And Surfactant Play On The Formation Of Emulsions And Biomass

The purpose of this experiment is to investigate the effects of various household products may have on bacterial growth. The bacteria that was chosen as a test subject is Micrococcus luteus, which is generally considered a harmless bacterium unless the host has a weakened immune system. Among the household products that a set of groups will test are common cleaning and disinfecting products such as rubbing alcohol, Betadine, Germ-X, Neosporin, liquid hand soap, and Lysol cleaner. In the experiment, the bacterium, Micrococcus luteus, is to be swabbed and grown in a Petri dish divided into four sections with agar in each. After that, a small circle of thin paper is soaked in the four household items. Then, a piece of parafilm

In part one (Influence of Crude Oil on prokaryotes) the following question will be answered; (1) which bacterial species in the Biello and Robinson articles gave off an impression of being best at separating the raw petroleum?

Abstract: This lab’s purpose was to see how different levels of yeast, distilled water, and sugar interact to affect the level of carbon dioxide evolved in fermentation. In this experiment we had two sections. The first section tested four test tubes with varying levels of yeast, glucose and distilled water for evolved carbon dioxide levels. The tubes were timed for 20 minutes. The amounts of solution in the test tubes are noted in the methods section of this lab report. The second section of the lab used three test tubes and flowed the same procedure except added spices. The levels of ingredients are also in the methods section. The main goal of this experiment was to see the effects of yeast concentration.

After labeling a new microtube with a marker, the culture tubes were removed from the incubator and observed under the ultraviolet lamp. Using a sterile pipette, the contents of the (+) culture tube were transferred to the 2 milliliter mictrotube and the (-) culture tube was disposed of. The (+) tube was then spun in the centrifuge at maximum speed for 5 minutes. Once it was removed from the centrifuge, a noticeable bacterial pellet had formed at the bottom of the tube and the remaining liquid was disposed of. After the

You can grow microorganisms in liquid media or on solid media. Most bacteria can be grown in labs as long as the media contain a source of the major nutrients; carbon, nitrogen, sulphur, and phosphorous. They may also need to have other nutrients. These nutrients are made into a broth and the pH and salinity can be adjusted. The nutrient broth is placed in test tubes, which are plugged with cotton wool, capped with foil and then sterilised in an autoclave, at 121c for 20minutes. These tubes are then cooled before they are inoculated. To prepare the streak plates we dipped an inoculating loop into ethanol then placed it in the flame until the loop glowed red. Still holding the inoculating loop by its handle we removed the lid from

Bioremediation is the process in which microorganisms such as bacteria, yeasts, molds, and filamentous fungi are introduced into the spills. The introduction of these microorganisms assists in altering and metabolizing various chemical compounds present in oil. When the microorganisms metabolize these certain compounds it significantly reduces the time it takes for the oil biodegradation to occur.

The thioglycollate broth showed that Clostridium sporogenes, Escherichia coli, Micrococcus luteus, and Enterococcus faecalis all showed growth that was throughout the tube but there was a slight inhibition of growth at the very top of the tube. Pseudomonas aeruginosa showed growth throughout all of the tube including the very top of the tube. With the TSA + glucose plate, all the bacteria but C. sporogenes was able to grow on the plate aerobically. For the plates that were grown in anaerobic conditions, only C. sporogenes, E. coli, and E. faecalis were able to grow on the plates.

Coupland here at Penn State. He is currently studying effects of these colloid suspensions with the discipline of food chemistry, applying his findings to applications of many sorts. In general, Dr. Coupland does not focus his research on creating or modifying a specific emulsified food, for example mayonnaise. Instead, he studies how the physical structures of food products are affected, which encompasses an overall study of emulsions in a immense array of foods. However, emulsions are ubiquitous in the world of food, coming in many shapes and sizes; therefore, Dr. Coupland has narrowed in on mainly oil in water emulsions, where water surrounds microscopic fat droplets to increase viscosity and alter various other physical properties. As a whole, the research being conducted by Dr. Coupland impacts food product, pharmaceuticals, etc. to a great degree, broadening the current viewpoints of the

In the 10-3 pasteurized sample, the plate exhibited 71,000 cells/mL. The results of the additional dilution samples contained too few colony forming units to count. However, in the 10-7 dilution, although the plate demonstrated 12 colonies, there should have been no colony forming units on this plate. The reasons for this could have been that this sample was contaminated from “double-dipping” the sample before dispensing it onto the plate or when using the pipette, it mistakenly was inserted in a higher concentration sample and then immediately to a lower concentration sample before it was dispensed onto the plate.

On the second day of the unknowns experiment, the TSA plates were observed and their results were recorded. The aerobic TSA plate had a medium amount of growth and the colonies were an off-white color, had irregular shape, undulate margins, and umbonate elevation. The anaerobic TSA plate had less growth, but there was still growth present; the colonies on this plate were transparent, had irregular shape, undulate margins, and raised elevation. The largest colony from each plate was sub-cultured and prepared to be smeared onto a slide for viewing under the microscope. Once the organism had dried, the slide was heat fixed and gram stained. The colony obtained from the aerobic TSA plate showed gram positive bacillus and gram negative bacillus. The colony that was acquired from the anaerobic TSA plate only had the gram negative bacillus. This showed that the gram positive organism was an obligate aerobe and that the gram negative organism was a facultative anaerobe. After the results of the gram stain, the gram negative organism was sub-cultured

Abstract: The purpose for this experiment is to see how much colonies will grow on the section four as it will be counted as growing bacteria on a 37C location. We as a individual were counted as our control with our unwashed hand and treatment once we did the steps of washing our hands. The handwashing procedure is a procedure for nurses using hand soap to kill bacteria then you had before in your hands. We followed the steps by first taking any jewelry off and having our hands and forearm clear from any clothing. Second, we covered our hands with soap and then rub the soap from palm to palm twice. Afterwards, we did the procedure but for the back of your hands and do the same steps. Once we had our treatment ready, we left it in a location

Another purpose of this experiment is to stress the importance of knowing the identity of a microorganism. Knowing the species of microorganism present in a sample provides a

The purpose of the two experiments was to determine the fundamental effects that temperature has on the growth and survival of bacteria. During the first experiment five different bacterial broth cultures of Escherichia coli, Pseudomonas fluorescens, Enterococcus faecalis, Bacillus subtilis and Bacillus stearothermophilus were individually incubated at temperatures of 5, 25, 37, 45 and 55°C for one week in an aim to distinguish the effect temperature has on growth and survival of the five different species. After one week they were observed for distinguishable changes by the turbidity showing an indication of bacterial growth, or the clarity an indication of no survival.

A fresh pipette was used to transfer 0.5ml of broth culture of E. coli, to be inoculated, into a tube of molten agar previously boiled to drive off oxygen. The tube was then rolled to distribute the bacteria and allowed time for the agar to harden. The tube was incubated at 37oC for 48 hours. The procedure was repeated for broth cultures of the bacterium Clostridium sporogenes and B. Subtilis.

Most industrial microbial processes are aerobic, and are mostly carried out in aqueous medium containing salts and organic substances; usually these broths are viscous showing non-Newtonian behavior. The rate of oxygen transfer to the cells is one of the limiting factors in the product formation. It is known that increasing the rate of oxygen transfer to the cells can enhance the rate of product formation. Gas holdup and volumetric mass transfer coefficient are mostly used to characterize oxygen mass transfer. Oxygen transfer is strongly influenced by