The Effects Of Mixed Bacterial Culture And Surfactant Play On The Formation Of Emulsions And Biomass

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The experimental results in this experiment were similar to, but not exactly the same as those outlined in literature. Primarily, examination of the demonstration material was relevant in helping understand the role that a mixed bacterial culture and surfactant play in the formation of emulsions and biomass. Preparing the control treatment (no inoculum, no surfactant) was important because it established a method of comparison of the remaining experimental treatments and assessing whether or not the results obtained were rational. The experimental flask that was prepared as part of this experiment contained no inoculum and had surfactant addition. As indicated by the observations, demonstration flasks that contained surfactant had…show more content…
Next, the demonstration flasks that were not inoculated did not have biomass adhering to the edges of the flask. Evidently, microbial growth could not have occurred in the flasks that were not inoculated, therefore biomass formation was inhibited in these flasks. Moreover, the degradation of hydrocarbons into simpler products did not occur in these flasks. Again, this is attributable to the lack of microorganisms in these flasks. Biomass was present in the two flasks that were inoculated by a mixed bacterial culture. Most importantly, however, biomass formation was most prevalent in the flask with mixed bacterial culture inoculum and surfactant addition. This observation aligns with the results outlined by Van Hamme et al., (2000), who noted that the addition of a surfactant to a mixed culture resulted in higher biomass levels throughout the incubation period (109 CFU/ml) than compared to a mixed culture containing only crude oil (108 CFU/ml). Moreover, the addition of a surfactant resulted in a 48 hour growth phase, as opposed to a 24 hour growth phase in the absence of surfactant (Van Hamme et al., 2000). Next, streak plates of the demonstration flasks were prepared. As was expected, demonstration flasks without mixed bacterial culture inoculum did not show signs of colony growth on the streak plate. In contrast, the streak plate that was prepared using the experimental flask (no inoculum, surfactant
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