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The Effects Of Ultraviolet Radiation On Survival Of Yeast Cells

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The effects of ultraviolet radiation on the survival of Saccharomyces cerevisiae
Whitney Jones
With Riley Allen, Josh Tesema, and Maryam Riaz
Dr. David Barnes
December 2, 2014

Abstract
The lab group conducted three separate experiments to test the effect of ultraviolet radiation on the survival of yeast cells. These experiments are significant because ultraviolet radiation can damage the DNA of human skin cells and eventually lead to skin cancer. The yeast cells were prepared and then exposed to UV for various increments of time. The first experiment was a comparison of a wild type strain versus an unknown type. The hypothesis was that if the unknown yeast has better adapted to ultraviolet radiation, more unknown cells would survive. The wild-type strain survived substantially more than the unknown. The second experiment introduced “protections.” The group covered the yeast samples with a thick black film or a sample of distilled water. More cells under the black film outlasted the cells under the distilled water. The third experiment covered the samples with a thinner, red film or a general sunscreen with SPF 70. More cells covered by the sunscreen lived than those under the red film.

Introduction
Ultraviolet light is present in sunlight. Generally the wavelengths are too short to be seen in the visible spectrum, it is present in sunlight, tanning beds, and black lights. (Lynch and Livingston, 2001) Earth’s atmosphere blocks

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