The effects of ultraviolet radiation on the survival of Saccharomyces cerevisiae
Whitney Jones
With Riley Allen, Josh Tesema, and Maryam Riaz
Dr. David Barnes
December 2, 2014
Abstract
The lab group conducted three separate experiments to test the effect of ultraviolet radiation on the survival of yeast cells. These experiments are significant because ultraviolet radiation can damage the DNA of human skin cells and eventually lead to skin cancer. The yeast cells were prepared and then exposed to UV for various increments of time. The first experiment was a comparison of a wild type strain versus an unknown type. The hypothesis was that if the unknown yeast has better adapted to ultraviolet radiation, more unknown cells would survive. The wild-type strain survived substantially more than the unknown. The second experiment introduced “protections.” The group covered the yeast samples with a thick black film or a sample of distilled water. More cells under the black film outlasted the cells under the distilled water. The third experiment covered the samples with a thinner, red film or a general sunscreen with SPF 70. More cells covered by the sunscreen lived than those under the red film.
Introduction
Ultraviolet light is present in sunlight. Generally the wavelengths are too short to be seen in the visible spectrum, it is present in sunlight, tanning beds, and black lights. (Lynch and Livingston, 2001) Earth’s atmosphere blocks
Different types of environments were encountered during that period of time. Since some of those environment didn’t receive as much radiation, “natural sunscreen” could have been harmful. The skin had to adapt to the environmental conditions that the new different regions had to offer. Recently, the evolution of skin color has been influenced by cultural behaviors. For example, people living around the Arabic Peninsula tend to be covered by wearing protective clothing. If they didn’t wear protective clothing perhaps their skin would start to darken. Towards the end of “Skin Deep,” Jablonski and Chaplin state that our survival depends on of the ability of skin color to adapt to different types of environments.
Saccharomyces cerevisiae, also known as baker’s yeast, is eukaryotic fungi (Nakasono, 2003). When yeast is fed, it uses oxygen and a sugar source to produce energy (ATP), and CO₂, the way humans do (Nakasono, 2003). Yeast undergoes aerobic respiration which involves oxygen.
We hypothesize that the time exposed to tanning beds, increases the risk of skin cancer. This hypothesis was a way to understand the statement, tanning beds give more harmful UV radiation rays in the shortest time, then being in the sun for the same amount of time. With this we emphasize that tanning beds are bed.
Dermatologists, physicists, and scientists conducted a workshop of UVA treatment, and the workshop’s findings are presented in the New England Journal of Medicine. A previous president of the American Academy of Dermatology wrote an article titled “UVA1 is Often A1” to discuss their findings. The workshop found that medium or high-dose UVA1 works well with morphea, urticarial pigmentosa, atopic dermatitis, dyshydrotic dermatitis, subacute pruigo, and systemic lupus erythematosus. These diseases are treated by UVA rays because the wavelength is longer, which means there is less energy delivered to the skin and the greater penetration of photons (Dahl, 2012). Dermatologists even use sunbeds with higher intensity than regular tanning beds in their offices. For example, Windsor Dermatology uses four different types of light therapy to treat the skin condition psoriasis. One type of light therapy is that of UVA. The anti-inflammatory properties of ultraviolet light aid in slowing the growth of psoriasis effected skin
UV radiation, such as that from the sun can be very harmful. It has been shown to cause many different mutations within cells, leading to issues for the organism such as death or disease. One of the most prevalent sources of UV radiation for humans is the sun. It’s very important for us to know the extent of cellular damage that can be caused by this radiation, as to know how harmful the sun’s rays are to us as humans. One way that the damage caused by the suns radiation can be tested is through the model organism yeast. For this lab, we exposed two different strains of yeast to UV radiation to test its affects. One strain was able to self-repair, while one was genetically altered so that it could not. Observations were recorded at
Whether swimming at the beach, picnicking at a park, or snowboarding in the mountains, our skin is continuously exposed to the sun’s ultraviolet rays as we go about our daily lives. The skin is beneficial as it protects our body from the outside elements including keeping out wind and water, dirt and dust, and all types of germs (Claybourne; 56). The topic of my experiment is to determine which SPF strength of sunscreen - SPF 30, SPF 50 or SPF 70 - is the most effective in blocking out the sun’s UV radiation. The purpose of this investigation is to observe, compare and determine whether the SPF label on sunscreen bottles are really effective in blocking out the amount of the sun’s UV rays, or if it is just a marketing gimmick to sell more products. This investigation benefits society because knowing which strength of SPF is the most effective in protecting against UV rays is useful to know when shopping for sunscreen, especially for those who spend a lot of time outdoors.
We hypothesized that the amount of UV radiation exposed has a negative correlation with yeast genetic stability, which ultimately determines the number of yeast colonies. In this experiment, yeast cells were treated with UV radiation, protected by photoprotective agents with different strength (in ascending order: Sunscreen #2, #1, aluminium foil). After letting it grow for seven days at 37 degrees, some grown colonies were counted to determine the effectiveness of photoprotective agents. After comparing collected results with the controlled group, we conclude that sunscreen #2 absorbs nearly zero UV radiation (0 yeast growth). Sunscreen #1 has some photoprotective effect (observed yeast growth). Theoretically, aluminium foil should be the
In the United States, more cases of skin cancer have been diagnosed than any other type. It is estimated that about 2 million of these cases arise every year, and can be prevented significantly from utilizing sun protection.
It is estimated that one out of every five Americans will develop skin cancer in their lifetime. People in the U.S. are diagnosed with skin cancer every day. Women are at higher risk of developing skin cancer than men, due to their recreational activities in the sun and over exposure to tanning beds. Doctors believe if preventive measure are taken, this can prevent some skin cancers from developing. Things like seeking shaded, especially between 10 AM and 4 PM, avoid tanning and UV tanning beds, Cover up with clothing, wear a trimmed hat, and UV-blocking sunglasses will greatly reduce chases of developing skin
[01.03]Sue conducts a series of experiments to investigate the effects of UV radiation on bacteria growth. In order to verify and strengthen her findings, she asks another scientist to conduct the same experiments in an independent investigation. The results of the second investigation are expected to be
Ultraviolet radiation is invisible rays that are part of the energy that comes from the sun. For example; Ultraviolet radiation can burn the skin, such as a sunburn and cause skin cancer (Medicinenet). The ozone layer is a layer of ozone (a colorless unstable toxic gas) in the upper atmosphere that prevents dangerous radiation from the Sun from reaching the surface of the Earth (Merriam-Webster). In correlation of the two together, we need the ozone layer to protect or animals and our plants from the ultraviolet radiation. Without the ozone layer being there it could damage the immune system to animals and humans, leaving many with skin cancer and eye cataracts (eco-action). The ozone layer is also important because it will have a negative
There was no difference between the control plates which proves that the lid on the plate serves as significant protection, potentially shielding the bacteria to the mutagenic effects the UV light. All three plates were incubated at 25 degree Celsius. The first Control plate showed lots of red and furry growth and the original pattern of swiping was observed in all the quadrants. The second control plate was also observed to have furry and pigmented red growth in all the quadrants, despite being exposed to UV light due to the plate lid during exposure. The experiment plate showed the most interesting results, in the 10 second UV exposure quadrant the bacteria had red pigmented growth but it is speckled, pinpointed and the original swipes were not observed. Moreover the growth seemed not as furry and robust as the control plates. The quadrant exposed to 20 sec of UV light showed significantly less growth than the 10 sec quadrant and the colonies are pinpointed with several beige colonies in between the red speckles. Original swipe pattern of the bacteria was not observed in this colony. In the quadrant exposed to 30 seconds of UV light, the differences are even more significant as the colonies are few and far in between and only several pinpoint red colonies found with a few beige colonies and some colonies with red centers surrounded by beige. The effects of UV light are very drastic in the 40 second quadrant as the growth is severely diminished with only a
Every hour at least one American will die of skin cancer caused by exposure to the sun. These statistics are alarming and most people are unaware that the sunshine that we all love so much is in fact a threat to our health and good looks. It is up to us to educate ourselves and our children on the harmful effects of sun exposure on the human body like premature aging, physical damage to the body, and cancerous diseases.
It has been proven that the Earth’s atmosphere blocks 98.7 percent of the sun’s UV rays. The other 2.3 percent that we are exposed to, have both beneficial and harmful effects. We do need to focus on the benefits, because we have way more of them. The only harm it does to our body is skin cancer, which by being more careful we can protect from. The
Natural and artificial light have varying properties that affects their capability and appropriateness these properties regularly impact their surroundings. Natural light comes in a spectrum of colours, which are the visible rays. Also on the colour spectrum are shorter wavelengths, named ultraviolet rays and higher wavelengths, known as infrared rays. Both of these are not visible to us. Exposure to the sunlight can help