The Field Of Cosmetics And The Treatment Of Injuries, Skin, And Hair

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Today, the field of cosmetics and the treatment of injuries, skin, and hair ailments have gained the attention of doctors all over the world. While multiple treatment methods have been examined, preference remains for the safest and the most effective method. Recently, treatment with PRP has been established as an effective medical treatment in many fields, such as oral surgery, neurosurgery, cosmetic surgery, sports medicine, and orthopedics. Platelets are one of the essential components found in blood, known best for their importance in coagulation. They contain proteins known as growth factors that play an essential role in healing damaged tissue (Lubkowska et al., 2012). The normal range of platelets in healthy human blood ranges from…show more content…
Despite these variations, all protocols follow the same sequence: blood sample collection, a soft spin for separating blood components, followed by a hard spin for concentrating the platelets, in the presence or absence of anticoagulants, and growth factor activators.
Kececi, Ozsu, and Bilgir (2014) examined the effect of the number of spins on the platelet recovery during centrifugation. Both single and double centrifugation methods were used to prepare the PRP. Two sets of blood samples were drawn from 10 adult New Zealand rabbits. The blood samples were added into 10% sodium citrate and centrifuged; The first group was single centrifuged at 160 G for 6 minutes at room temperature, while the second group was double centrifuged, the first spin was at 160 G for 20 minutes at room temperature, followed by the second spin at 400 G for 15 minutes. Average platelet amount collected from the second group (1,986,875 ± 685,020/μl) was greater than the first group (781,875 ± 217,693/μl). According to Amable et al. (2013) different centrifugal forces have been applied to obtain pure PRP from the blood samples obtained of healthy donors (4.5 ml of WB), using a refrigerated high speed centrifuge, with speeds reaching up to 30,000 rpm (rotation per minute). Since the lower centrifugal force (less than 300 x g) did not release a sufficient amount of PRP, 300 × g for 5 minutes at 18°C

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