The Field Water Samples

This experiment was centered on metabolic and biochemical testing procedures. The rationale of performing these tests was to distinguish six different microbes from one another and to compare how their metabolic and biochemical processes differ from species to species to determine the unknown sample.

In part II of the lab six small glass tubes were obtained in a test tube rack. Ten drops of distilled water were then added to test tube 1, five drops to tubes 2-4, and no drops in tubes 5 and 6. Five drops of 0.1M HCl were added to test tube 5 and five drops of 0.1M NaOH to test tube 6. Five drops of enzyme were then added to all tubes except tube 1. Tube 3 was then placed in the ice bucket and tube 4 was placed in the hot bucket at 80-900C for five minutes, the remaining tubes were left in the test tube rack. After the five minutes five drops of 1% starch was added to every tube and left to sit for ten minutes. After ten minutes five drops of DNSA were then added to all the tubes. All the tubes were then taken and placed in the

In The Color of Water, author James McBride explores all of the hardships and experiences that lead to the inevitable aspect of life; change. Writing this autobiography and memoir for his mother allows readers to understand what he and his family dealt through. James holds an insane infatuation for his mother’s past and went on to find out who she was in order to understand her constant proclivity to keep her previous life confidential. Rachel Shilsky remained mute and hopeless throughout her childhood. Unlike Rachel, the life she lived as Ruth was that of a woman with power and a voice that fought against mistreatment. It was imperative for Ruth to destroy any memory of Rachel Deborah Shilsky and take on a new identity in order to start afresh. James McBride focuses the book on the high levels of deprivation his mother went through. His thoughts on his mother’s way of life change when he comes face to face with the life she lived. As Ruth faces more challenges throughout her life she grows as a person who no longer fears anything, and finally relinquishes her past to James.

“Waterlily” by Ella Cara Deloria begins with a woman named Bluebird giving birth to a child named Waterlily while away from the caravan. The story shifts to a memory from Bluebird’s childhood about how she lost her family to a possibly raid or attack by another tribe while gathering supplies. However, the only other living relative happens to be her grandmother. Later, Bluebird and her grandmother set off to find a neighboring tribe. Eventually, Bluebird meets a young man named Star Elk. Bluebird goes on to marry Waterlily’s father, Star Elk, who happens to be a terrible husband. However, Bluebird remarries Rainbow. The story finally shifts to focus solely on Waterlily. During her childhood, Waterlily and her family are invited to attend a Sun Dance. Also, she witness her cousin, Leaping Fawn, participating in a Virgin’s fire and learning the proper way to accept a marriage. Eventually, Waterlily is brought by Sacred Horse because she felt that she must fulfill these obligations due to her family. However, Waterlily has a hard time adjusting to her new life and the new role she has to play. Waterlily begins to miss her family and people but she finds a sense of comfort with Red Leaf’s parents. She is finally able to relax with people she can relate to. Back at camp, an outbreak of smallpox spreads throughout the tiyospaye due to buffalo blankets. Sadly, Sacred Horse catches the sickness and requests to die alone. Leaving a pregnant Waterlily without a husband. Waterlily and

For the temperature test each bacteria was placed on a nutrient agar and incubated for either 10, 20, 30, 40, or 50 degrees Celsius for 48 hours. During the pH test, each organism was placed on four agars varying in pH level from pH 2, 4, 6 and 8 and incubated near 37 degrees Celsius for 48 hours. For the osmotic pressure test, each organism was placed on four agars one each containing 2%, 5%, 8%, and 11% NaCl concentration levels. These were incubated near 37 degrees Celsius for 48 hours. The results of the tests are recorded in Tables 1, 2, and 3. All tests were performed according to the instructions provided in Leboffe & Pierce(1). The biochemical tests used on both unknowns and the ubiquity are:

[1] Ruth's memory of her childhood includes the explanation of her drastic separation from her family, that explains why she always avoids the topic of her family when it's brought up. Ruth is hesitant to remember the memories of her painful past.

The book, The Color of Water by James McBride presented many aspects of cultural diversity from different individuals to groups of people. The book is an autobiography about James McBride’s mother, Ruth. The author thoroughly explains what Ruth had to go through during the time where diversity amongst individuals was clearly avoided. In addition, it explains James’ life after the death of his father and the ups and downs he encountered. After reading this book, I now have a better understanding of how important it is for people accepted the differences in each other and accept that we are a country of cultural diversity, not divided groups. The analysis of this book will thoroughly explain the positive and

The Color Of Water: A Black Man's Tribute to His White Mother, by James McBride is a Multiple Point-of-View novel in which a white mother and her black son deal with a recurring question: Is it possible to reject certain parts of one's Identity? They each find themselves lost in a world of racial and religious prejudice. Determined to start a new life, the mother, Ruth, leaves home with one goal: to leave behind her old identity. While searching for a new life, Ruth learns that her own view on topics such as race and religion are sometimes more meaningful to her life than the traditions she had once grown up learning. She uses the method of rejection to not only push her past behind her, but to also open up a world of new opportunities for her new identity.

The beautiful town of Stillwater is located on the St. Croix river in the St. Croix River Valley and is known as the Birthplace of Minnesota. The early days of Stillwater were uncertain and uneasy as the land was fought over for many years. The coveted land transitioned from being part of Wisconsin territory to being included in Washington county in 1938. All the while, lumbering interests drew many to the area and white settlers took up residence in Stillwater. One former soldier, Indian trader, Justice of the Peace and promoter, Joseph Renshaw Brown had been in the area since 1820. He had set up a store and farm at Grey Cloud Island and kept a warehouse at the head of Lake St. Croix to supply upriver fur trading operations. This warehouse, which was located where North Stillwater stands today, grew into a small village that he called “Dacotah”. In 1840 the first ferry was licensed to operate across the St. Croix.

The SIM media contains nutrients, iron, and sodium thiosulfate. Hydrogen sulfide can reduce to sulfur, and if this occurs, then the hydrogen sulfide will produce a black precipitate by combining with iron to make ferrous sulfide. If the broth does not turn black, then the test is negative. Kovac’s reagent was added to the SIM media to test for indole. Tryptophanase is an enzyme that hydrolyses tryptophan. The products from this reaction are indole, pyruvic acid, and ammonia. The Kovac’s reagent that was added should form a red compound in the SIM media to have a positive result. If the media does not turn red then the test is negative. Also, in the motility test, the test tube was stabbed with the unknown, and growth around the stab line would indicate a positive test result. The MR-VP test contains two test and two test tubes. The MR and VP were subcultured in separate test tubes, but the same broth was used for both tests. Adding methyl red (pH indicator) to the broth is the indicator for the MR test. If the bacterium uses mixed acid fermentation pathway and stable acidic products are made, the acids will make an acidic environment. When methyl red is put into the medium, the broth will stay red if there are acidic products present. The VP tests shows bacterium that use the butylenes glycol pathway and have a product of acetoin. The acetoin that is

The test tube was labeled with the bacteria identifying number. The cap on test tube was removed, and the lip of the test tube was flamed. Next, the Bunsen burner was used to sterilize the inoculating loop. Then, bacteria were picked up from the working plate with the loop and the agar was inoculated. The loop was then re-flamed. Finally, the plate was placed in the 37˚C incubator and left to sit for 48 hours and any changes in color were observed. A negative result appears green, and a positive result appears blue. This is because it tests for the organism’s ability to use citrate as its sole source of carbon, and if it does then it produces ammonia and ammonium hydroxide which make the medium basic, changing the green agar blue (Leboffe & Pierce,

6. The disks in the 0.00% solution were transferred to an agar plate held next to the blue flame using the sterilized tweezers. Excess disinfectant was removed from the disks by wiping on the side of the well of the spotting tile. When the 5 disks were positioned (refer to Figure 1 below) the lid was replaced and sticky taped down. A label was added indicating the concentration of disinfectant.

-Sterility: Each sample will placed in a sealed bag, preventing the introduction of extraneous bacteria.

Laboratory ABC uses both glass pipettes (1mL to 5mL) and disposable plastic pipette tips- yellow (20μL), blue (1000μL) and white (5mL). Glass pipettes and white pipette tips are currently washed and reused. Blue and yellow tips are discarded.

A sterile pipette was used to add 0.1ml of E. coli culture to the pH 3.0 tube. This was then repeated for the tubes at pH 7.0 and pH 9.The tubes were then incubated at 37oC for 48 hours. This was then repeated for saline culture of Saccharomyces cerevisiae but incubated for 72 hours at 25oC.