The Formation Of Cellulose Fibrils Essay

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Other genes like chvA, chvB, pscA and att regulate the formation of cellulose fibrils which are required for the first step of weak and reversible attachment. Cellulose fibrils help the bacteria to attach to the wounded parts of the plant and also anchoring to each other forming a microcolony.
VirD4 protein and 11 VirB proteins make up type IV secretion system for the transfer of T-DNA and several other Vir proteins like VirE2, VirF etc to the host cell. VirD4 also promotes interaction of the processed T-DNA/VirD2 complex. As discussed earlier the T-DNA/VirD2 complex in turn transforms into the T-strand that is then transferred into the plant cell. Most VirB proteins help either in forming the membrane channel or serve as ATPase to provide energy. T-pilus is then formed by VirB2 ,VirB5, VirB7, VirB2 as they are the major pilin protein.

So the first major step in the transfer of T strand is the formation of a long flexious appendage known as the T-pilus (Bulgakov, 2000) . The detection of presence of acetosyringone and other substances activates the expression of 11 genes in VirB operon which are responsible for the formation of the T-pilus. Other products of VirB genes like VirB6, VirB7 etc are used to transfer other subunits across the plasma memebrane.
Now the T-DNA is delivered as a single stranded DNA molecule coupled with the VirD2 protein in the plant cell. The T-DNA is then integrated into the plant chromosome and are expressed by plants transcriptional machinery.

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