The Immune System Of Rats

961 WordsMay 2, 20164 Pages
In this study, the immune system of rats was systemically suppressed with DEX to evaluate the effects on VEGF expression and leukocyte accumulation within the ovary. Half the rats received DEX to transiently suppress their immune system, while the remaining rats were exposed to the appropriate vehicle (Control). To prevent possible bacterial infection, DEX-suppressed rats received tetracycline, an antibiotic. Since DEX-suppressed rats receive tetracycline, Control rats also received tetracycline as an appropriate control. A total of 6, twenty-one day old female Sprague-Dawley rats (Charles River Laboratory, Wilmington, MA) were utilized as follows: 1) Control (n=3 rats): Rats received rat chow and tetracycline (500mg/liter) in their water for 8 days, starting from 21 days of age to 29 days of age. 2) DEX suppressed (n=3 rats): Rats received rat chow, DEX (.024mg/liter), and tetracycline (500mg/liter) in the drinking water for 8 days, starting from 21 days of age to 29 days of age. On 30 days of age, the rats were euthanized via carbon dioxide asphyxiation to collect ovaries and prepare frozen histological sections for VEGF expression and macrophage accumulation (immunohistochemically). Immunohistochemistry for Macrophages within rat ovaries: Frozen tissue sections of ovaries were air-dried, fixed in cold ethanol for 10 minutes at 4°C. Sections were rinsed 3 times, 5 minutes each in PBS containing 1% BSA. Sections were then blocked with 10% normal horse serum (Sigma,
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