Upconversion is a term that characterizes the conversion of lower-energy light, particularly in the near-infrared region, into higher-energy light within the visible spectrum. This occurs through a process in which the sequential absorption of multi-photon near-infrared light yields the emission of light that is of shorter wavelength[1]. This process is represented in Figure 1, which shows the emissions of light within the visible spectrum after excitation of various dopant ions by near-infrared light[1]. The upconversion effect has been observed primarily in the rare-earth metals of the lanthanides as well as yttrium, and scandium; however, some transition metals and actinides can also display this effect[2]. Lanthanide ions display special inner-shell arrangements of 4fn 5d0-1, which allow for unique energy levels and provide shielding from the outer shells[2]. Due to their various f-orbital configurations, lanthanide ions are able to display very sharp luminescence emissions via intra-4f or 4f-4d transitions[2,3]. The importance of the lanthanide ions is evident in several applications including applications in lasers, bioprobe applications, optical imaging, and photodynamic therapy [2,3]. This is due to their fluorescent properties, which include: resistance to photobleaching, monochromaticity, large Stokes shifts, narrow bandwidth, and long emission lifetime[2,3]. The direct excitation of the lanthanide ions is not very effective because they are limited by low
The purpose of this lab was to see which level of light (measured in lux) made Spinacia oleracea (Spinach) leaf disks float the fastest. Our hypothesis was that an increase in light intensity will decrease the time it takes Spinacia oleracea disks to float. If light intensity is increased, then the time it takes Spinacia oleracea disks to float will be decreased. The mean for the no light (0 Lux) sample and the low light (4 x100 Lux) sample was 1200 seconds with no standard deviation because none of the disks in these two samples floated. The mean and standard deviation for the medium light (110 x100 Lux) was 902 seconds +- 84 seconds. The mean of the high light sample (410 x 100 Lux) was 692 seconds with no standard deviation because only two Spinacia oleracea disks floated so there was no need to measure the variability of the data. The final results indicated that the highest light intensity led to the quickest rise of Spinacia oleracea disks, supporting our hypothesis.
The effects of light intensity and light wavelength on photosynthesis was observed in two different experiments and closer study of different pigments in spinach was observed. For the experiment where the effects of light was observed, it was found that a light intensity of 30 cm, wavelengths of blue and red lights show the greatest photosynthetic activity. The different pigments present is spinach were also observed and it was noted that though chlorophyll is the most abundant in plants, there are other pigments present as well.
Photosynthesis is the conversion of light energy from the sun into chemical energy. During this process light energy is absorbed by the chlorophyll molecule (a green pigment found in chloroplasts of plant cells) and is then used to convert carbon dioxide and water into simple sugars, glucose. The chlorophyll molecules however absorb different colours enabling plant growth as well as food production and by this it could be identified whether photosynthesis does occur or not. Blue and red light are the most absorbed and this is because they are colours that are most effective when driving photosynthesis. Green light is reflected which is why leaves appear green. Plant growth will occur the most under the red and blue light but not green. This is because red light wavelengths which are absorbed by the leaves of the plant will produce a hormone which stimulates budding and flowering of plants and blue light will promote photosynthesis which increases the rate at which it occurs.
The sunlight photons allows for the infrared photons not to settle all the way down.
Some significant similarities and differences between lanthanides and actinides are observed. The two rows that are generally placed underneath the main periodic table are called the lanthanides series and the actinides series. These two rows are produced when electrons are being added to f orbitals.
Fluorescence imaging is visualization of fluorescent probe labeled processes or structures with the help of various fluorescence imaging techniques such as time-lapse microscopy, confocal microscopy, fluorescence microscopy, etc. In recent years, fluorescence imaging has received much attention especially in the field of biology and medicine due to the increasing availability of fluorescent dyes, proteins, and probes which provides ease to the noninvasive study of many biological processes. Fluorescent labeling using usual organic dyes, fluorescent proteins and lanthanide chelates are the preferred methods due to its low cost, availability and ease of use. However, they posses some inherent drawbacks such as poor photochemical stability, short
The purpose of this lab was to observe and reserve the emission spectra of various elements in which hydrogen was the element that was emphasized on. More specifically, the frequency, initial energy level, and change in energy of the element hydrogen was determined by the wavelength of the light in the hydrogen atom.
When atoms or ions in the ground state (the lowest allowed energy state of an atom, molecule, or ion) are heated to high temperatures using a Bunsen burner, some of the electrons may absorb enough energy to move into an excited state (any quantum state of an atom that has higher energy than the ground state)
Complexation of porphyrin with the metal will cause the shifts of Q bands in the UV-Vis spectra in the range of 500-700 nm as shown in Figure 2.7. This can be observed by the disappearance of two Q bands out of four as the porphyrin undergoes the change from D4h symmetry to D2h symmetry (Rita Giovannetti, 2012). The Q-bands were denoted as α and β. The coordination of porphyrin to Ni(II), Sn(IV) or Pd(II) which can form stable square planar complexes generally consists of a higher α peak (Figure 2.7 (A)), while the coordination of porphyrin to Cd(II) which forms a complex that can easily be displaced by protons will show a higher β peak (Figure 2.7(B)).
● Infrared light comes from the things that we can see and feel everyday. For
To answer the questions previously asked, Lee et al conducted an experiment using several methods, which include fluorescence resonance energy transfer (FRET), two-photon microscopy, photostimulation, and
The first hypothesis stated for this lab was that our wavelength would be around 500 nm for the colored light. The second hypothesis stated was that the full spectrum light will have a better outcome for both photosynthesis and respiration compared to the green lights results. Throughout this experiment both of this hypotheses were supported. The first hypothesis was supported because our colored ended up being533.8 nm which represents the green light. Therefore, the second hypothesis was supported because the full spectrum light did have more linear results compared to the usage of the green light. The full spectrum light had better results with the processes of photosynthesis and respiration. The full spectrum light covers all aspects of
Light spectra of the sampled habitats of studied species. a Upward light in air; b underwater upward light; c underwater sideward light. Light spectra shown are the geometric means of the measurements at the respective collection sites. Red: habitats of P. h. himantegus; Blue: habitats of P. h. chii.
This IR spectrum sets forts wave numbers in the X-axis and the transmittance at the Y-axis. This spectrum elucidates the gap in the wavenumbers of the absorptions in the transmittance in addition to the wavelengths of the infrared light. As you can see the peak at 2300 to 2400 in the IR spectrum indicates the relation of the carbon double bonded oxygen.
Genesis 1:3). This sentence can be the most famous sentence that has ever been written. When light was created, the faith of the universe, earth and humanity was completely redesigned. The control of our universe was given to its owner, light. We wereare born into a universe that light governs us. However, we couldn’t realize it in a short span of time. After that moment, the perception of humanity towards light, a mysterious phenomenon, has altered a lot; nevertheless, light has never lost its meaning of being fundamental for anything related to human, conversely, we discovered beyond what we knew about it.