The Isolation And Identification Of An Unknown Bacteria

Abstract and Introduction Microorganism identification plays a very critical role in the medical significance of modern medicine. One example of reasoning behind it can be for the detection of a disease causing organism in a patient, so it can be certain that it will be appropriately treated. Numerous species of bacteria can appear to share identical morphology (spirilli, cocci, rods), when looking through a microscope. There are many methods and biochemical tests that can be performed to differentiate

The identification of bacteria is a fundamental objective of microbiologists. It is essential to distinguish specific bacterial properties to understand the environment, physiology and disease. As new bacterial species emerge and existing ones evolve into different strains, it is imperative that microbiologists continue to isolate bacteria from the field, identify their findings and research newly discovered forms. Their discoveries can then be used to evaluate the types of microbial life that may

For many years the identification of microorganisms has been important in the world of medicine. It is essential or correct disease diagnosis in patients and for proper treatment. Knowing the correct identity and characteristics of microorganism is crucial when disease outbreaks occur in populations, also knowing how humans can benefit from microorganisms is important; many can be used in making certain foods or antibiotics. The purpose of this study project was to carefully isolate and identify

Identifying Two Unknown Species of Bacteria Materials and Methods Week 1, Day 1 (10 November 2000) The first day an unknown sample was assigned to each group of students. The first test applied was a gram stain to test for gram positive or gram-negative bacteria. The morphology of the two types of bacteria was viewed under the microscope and recorded. Then the sample was put on agar plates using the quadrant streak method for isolation. There were three agar plates; one was incubated

The strategy used to determine the unknown organism was to first perform a gram stain to determine if the organism was either a gram negative or gram positive bacteria based on the color of cells. A TSA plate was inoculated to streak for isolation and purity. After colonies were incubated characteristics of colony morphology were recorded and a TSB broth and TSA slant were inoculated to determine characteristics of growth in broth and slant. Another TSA plate was inoculated to determine growth

MCB 3020L- Section 018 Unknown #16 Abstract This report contains the background information on gram positive and gram negative bacteria, which will aid in understanding the use of specific laboratory experiments to distinguish between the two types of bacteria. Included are the materials and methods used to identify the gram positive and gram negative bacteria and methods which also differentiate between microbes of each group. The implications of the methods of identification used are also described

test was also negative. The Novobiocin test was sensitive with a forty millimeter zone of inhibition. The effects of oxygen on my unknown bacterium was facultative anaerobic meaning the the bacteria was distributed throughout the tube but mainly in top of the tube . In the catalase test it came out positive so there was some bubbling. Introduction In the unknown lab we had to find out what bacterium we had through a series of tests that we had previously done as a class. The

specimen was an unknown sample of a mixed culture of two different species of bacteria. The first step that was taken was obtaining a pure culture of each species of bacteria by isolating one species from the other. Once isolation was complete, the isolated cultures were tested using procedures and tests that had been performed during previous lab sessions. Each mixed culture that was tested had one gram positive and one gram negative bacterial species. The possible species of bacteria that could have

The purpose of this experiment was to identify two species of bacteria from an unknown mixed sample out of a possible twelve strains. With the intentions to correctly identify the unknown bacterium, a plethora of test were executed and documented, yielding the appropriate results for that particular sample. In this experiment, many methods were critical in the identification of the unknown. A dichotomous key, streaking and staining techniques, microscopy, differential and selective media, colony

Determination of Unknown Microorganisms Unknown Numbers 1 & 2 Justin Lucas MCB3020L 10561- M/W: 2-4pm April 21, 2015 Introduction Microorganisms are ubiquitous and play a large role in the environment. Microorganisms help some other organism in symbiotic relationships and they can also harm other organisms by causing them disease. Tests have been developed to help determine the characteristics of many microbes so that their special capabilities can be utilized for benefit. While these tests