The Level Of Light Intensity

Measuring the Rate Oxygen Production using an Oxygen Electrode Chamber in Photosynthesis of Spinacia oleracea with Varying Light Intensities

Have you ever really wondered how different variables can affect how plants go through photosynthesis? Well, in this experiment, the purpose was to see how various environmental conditions can affect the overall photosynthetic capacity of a specific plant. The factors, light, darkness, cold, and heat were applied to see how the different components would affect the photosynthesis on spinach plants. Each group was given a different factor to test. Out group was given the light factor. The hypothesis for this experiment is that when adding light as a factor, the light will affect the overall plant photosynthesis.

Introduction: Photosynthesis can be defined as a solar powered process that removes atmospheric carbon dioxide and transforms it into oxygen and carbohydrates (Harris-Haller 2014). Photosynthesis can be considered to be the most important biochemical process on Earth because it helps plants to grow its roots, leaves, and fruits, and plants serve as autotrophs which are crucial to the food chain on earth. Several factors determine the process of photosynthesis. Light is one these factors and is the main subject of this experiment. The intensity of light is a property of light that is important for photosynthesis to occur. Brighter light causes more light to touch the surface of the plant which increases the rate of photosynthesis (Speer 1997). This is why there is a tendency of higher rates of photosynthesis in climates with a lot of sunlight than areas that primarily do not get as much sunlight. Light wavelength is also a property of

In conclusion, the hypothesis is supported by the experiment. Only 2/10 disks floated to the top of the 0.1% NaHCO3 solution, while all 10 of the disks in the 0.2% NaHCO3 solution floated to the top. A potential follow-up experiment could be to test the affects of increased concentrations of carbonation on PS (Bagley et al., 2015). There was the possibility of human error and bias having impact on the experiment. When using syringes, the plunger may have been pulled too harshly and damaged some of the disks. This could have led to disks not floating to the top in the experiment. Another form of error could have been the use of disks that were cut from the veins of the leaf, which has less chloroplasts, meaning less process of photosynthesis happening, and result in the disks not floating to the

The rate of photosynthesis can be determined different ways. Because oxygen is a product of photosynthesis and the Elodea plant is submerged in water, the oxygen is released in bubbles that rise to the surface of the water in the beaker. In this experiment, the rate of photosynthesis for each degree of light intensity can be measured by counting the number of bubbles released every 30 seconds for five minutes at each distance. The rate is the number of bubbles released per minute.

In this lab, you will be experimenting with how different variables affect the rate of photosynthesis.

The aim of this practical was to test whether a Scarlet Globe Radish will grow higher in cm when watered using different types of water and the hypothesis was that scarlet globe radishes should grow higher when watered with sparkling water as it contains co2 however that hypothesis was proven incorrect. From results it can now be concluded that the control patch surprisingly produced the highest plants in terms of height with an average ground to leaf height of 2.2 cm while the sparkling water patch produced 1.86cm and the filtered water patch with an average height of 1.18. However, from research it was shown that sparkling water was more likely to make radishes grow at a faster rate. A faster growth rate would’ve made the radishes taller

This experiment demonstrates the effects of pH on the rate of photosynthesis by examining the behavior of leaf disks in different pH solutions under light. In this experiment, we used five different pH levels: pH 5, pH 6, pH 7, pH 8 and pH 9. These solutions were created using a combination of hydrochloric acid and sodium hydroxide. Spinancia olcerea or spinach, leaves were used in the experiment to examine the effects of pH on the rate of photosynthesis. The rate of photosynthesis was measured by counting the number of leaf disks that rose to the surface of the solution after each minute. In acidic solutions, the rate of photosynthesis increased while in basic solutions, the rate of photosynthesis decreased.

Elodea Canadensis, also referred to as American waterweed or common elodea, is an aquatic plant that is usually found in lakes, ponds or even rivers. Elodea helps support aquatic life such as young fish and amphibians by providing shelter and can be consumed as food by ducks and beavers. Elodea, like many other plants, relies upon photosynthesis to acquire its energy and to make food. To determine the importance of light energy in the process of photosynthesis, two Elodea plants were placed inside CO2 saturated water and exposed to different light intensities: one was under full light exposure while another was covered with mesh clothing to reduce the amount of light that it received; the solutions to this plants were then neutralized and compared to a control group which had no Elodea to see the amount of CO2 that each would have left after a period of an hour. The yielded results indicated that under high intensities of light, the Elodea plant photosynthesized and respired at the same rate; and while it was covered, it photosynthesized more because of an indicative decrease in CO2 levels. Therefore light energy is an essential aspect that affects the amount of CO2 that a plant can use in the process of photosynthesis.

When sodium bicarbonate is added to the water, the bicarbonate ion acts as a carbon source for photosynthesis causing the leaf disks to sink. As photosynthesis proceeds, oxygen is released into the interior of the leaf, which changes its buoyancy causing the disk to rise. Since cellular respiration is taking place at the same time in the leaf, consuming oxygen, the rate that the disks rise is an indirect measure of the net rate of photosynthesis.

The photosynthetic rates of Camomba Caroliniana, were effected by the color of light it was exposed to during the experiment. As seen in Table 1, Camomba Caroliniana that was exposed to green light had an average photosynthetic rate of 0.13 mL O2 produced/g/hour, which was lower than the photosynthetic rate of Camomba Caroliniana that was exposed to white light (0.19 mL O2 produced/g/hour). The data from table 1 can also be used to determined the reliability of the experiment, the standard errors for green and white light are relatively small, 0.02 and 0.01, respectively. This means that there is less variation in the experiment, but the experiment involving white light is slightly more reliable because its standard error is smaller. The 95%

The results were conclusive and differed slightly from previously expectations with the plants germinated with an absence of light growing on average 17.975 mm per day compared to the plants with exposure to light growing 6.965 mm less at an average of 11.01 mm per day. This trend was evident throughout the duration of the experiment however as the investigation continued it was clear the growth of both plant sets was beginning to decrease and slow. As shown in graph 2.0 and table 1.0 on day 15 the plants grown in the dark had reached a height of 175.9 mm and by day 17 they had only grown 1.475 mm and reached a height of 177.375 mm, compared to the growth between day 8 and 9 when the plants grew by 44.375 mm, a 48% increase, nearly doubling its height of 47.85 mm on day 8. The heights of both sets altered from each other for most of the experiment expect for day 10 when the heights only altered by 0.891 mm with the plants in the dark having a average height of 108.125 mm while the plants with light had reached a height of 108.944 mm.

Discussion The hypothesis for this experiment was, “If the light intensity increases, then more leaf disks will be floating after the 18 minute period.” This hypothesis was supported because at the end of the 18 minute period, the syringe that had the intensified light had more leaf disks floating than the syringes with less intense light shining on them. Throughout the entire 18 minutes, the syringe with regular water and the syringe with the bicarbonate solution and regular light intensity had no leaf disks float to the top. But the syringe with the bicarbonate solution and intensified light slowly had all 10 leaf disks float to the top.

In this study, the focus is on the effect of a reduced quantity of light being the intensity of the light and quality of the light being the wavelengths that the chloroplast isolate of Spinach beet (Beta vulgaris subsp. cicla) is exposed to, and whether the results indicated have a connection to the effect of the difference in lights in forests, and hence the effect on seedlings and plants underneath dense canopies and their ability to photosynthesis, therefore their photosynthetic activity. Together with investigating whether the intensity of the light or the quality has a greater effect on the rate of photosynthetic activity, was also the effect of varying densities of chloroplasts on the rate of activity.

The light loss factor is a major one and is commonly referred as LLF. It is simply the multiplying factor in which the light output can be changed mostly because of the environmental impacts as well the product degradation. The Lamp Lumen Depreciation which is commonly abbreviated as LLD, Lamp Dirt Depreciation (LDD), Luminaire Ambient Temperature Factor (LATF) as well as the Ballast Factor (BF) are among the common factors that widely affect the light output. These four factors are the major ones and hence the ones to mostly focus on.