The Metabolic Capabilities Of Cell Culture Models

Purpose: The purpose of this lab is to study the relationship between cellular respiration in the muscles, and muscle fatigue due to lactate fermentation in anaerobic respiration.

Another importance of animal testing is this testing makes large contribution in toxicity; a study of harmful effects of chemical substance on living organisms besides finding cure for human diseases (The MSPCA–Angell, 2013). In fact, Daston, Procter and Gamble made a clarification in their article of Alternatives in Reproductive Toxicity: A Way Forward, that animal tests are being relied on for the prediction of potential harm of chemicals which may affect human reproductive system. This is because, as some animals and human have identical characteristics in complex body system, animal tests that is conducted for evaluating toxicity of reproductive are designed to apply on the complete reproductive cycle. Those three authors also added that animal testing become a medium for good prediction of toxicity as animal body systems are as complex as human’s. Rather than testing the chemical substances on human which is used for predicting potential harmful effects on body system, animal testing is the most appropriate and safest medium to save human lives.

To be able to carry on metabolic processes in the cell, cells need energy. The cells can obtain their energy in different ways but the most efficient way of harvesting stored food in the cell is through cellular respiration. Cellular respiration is a catabolic pathway, which breaks down large molecules to smaller molecules, produces an energy rich molecule known as ATP (Adenosine Triphosphate) and a waste product that is released as CO2.

There are many processes that are needed to occur to produce something that help organisms live. Cellular respiration and fermentation are two process that are important to the survival of organisms. Cellular respiration is the way cells make ATP, which they need to survive. The process starts with the breaking down of glucose into other compounds that can be used by the cell. However, there are more steps in the process than just cellular respiration and how precise cellular respiration is depends on how much ATP can be taken from food particles in the body (Hill 646). Fermentation is mostly known in the world of beer and wine, but it also produces lactate in organisms. Fermentation is breaking glucose into separate components like water or carbon dioxide, much like that of cellular respiration. N’guessan and some peers did an experiment and they found that after fermentation had stopped, they had over 200 counts of yeast in the beer (N’guess, Brou, Casaregola, Dje 858). Under the

b) Janine made this statement: “… if your brain doesn’t get carbs—well, glucose, anyway—you get

small wad of absorbent cotton in the bottom of each vial and, using the pipette or syringe, saturate the cotton

Reinberg, S. (2013, July 31). Plastics Chemical BPA May Harm Human Fertility. Retrieved April 11, 2017, from http://www.webmd.com/baby/news/20130731/plastics-chemical-bpa-may-harm-human-fertility-study#1

The purpose of the Cellular Respiration lab was to identify if cellular respiration is sped up due to exercise. To conduct the experiment each student filled a beaker with 15 ml of water and three drops of bromothymol blue. Next each student blew into the beaker through a straw until the water mix turns yellow. When carbon dioxide is added too bromothymol blue it changes to a yellow color. Each student times how long it takes for the water to turn yellow, records the data, and then reset the lab. Subsequently the student would exercise for a total of five minutes and then repeating the lab, again recording how long it takes for the water mix to turn yellow.

Abstract: Metabolism is used to refer to all of the chemical reactions involving energy transformations in the cell of the body, for example cellular activity such as cellular respiration and its sub activtys like glycolysis, Krebs Cycle and the Electron Transport Chain all produce energy in the form of ATP and a byproduct of the energy production, heat is also produced . Although the cell is not 100% efficient thermodynamically, the energy in the form of heat is lost in the cell each time when energy is produced. Metabolic rates of an organism can be measured directly by the amount of heat lost from the body in a certain period of time. Another way an organisms metabolic rate can be measured is indirectly, by measuring the amount of oxygen that is consumed in a given period of time. In the experiment conducted, we needed to find the metabolic rate of an ectotherm. Using a goldfish (ectotherm) we calculated the metabolic rate by means of counting the number of operculum beats when three different goldfish where submerged in various temperatures. What was found is that the metabolic rate increased when the temperature increased and the metabolic rate decreased as temperature dropped.

The hypothesis of this lab was if yeast is exposed to glucose, fructose, lactose, sucrose and DI water, then the yeast will break down glucose at the fastest rate and produce the most CO₂. This hypothesis was supported by the results of the lab. The results showed that glucose produced the most CO₂ in comparison to the other solutions, supporting the idea that cellular respiration was happening the fastest in the glucose solution. As cellular respiration is occurring, CO₂ is being released into the air. The carbon in the CO₂ is coming from carbons in the reactants of cellular respiration and are released to get rid of excess carbons. By looking at the rate at which carbon dioxide is being produced, the rate of respiration can be identified because the release of CO₂ is a product of respiration

If the pollutants are altering body cells certain parts of the body may not grow properly and more fat would be produced where it doesn’t belong. When these “…chemicals [are present] in the mom’s blood, such as DDE (a by product of the now widely banned pesticide DDT, which lingers in the environment decades later, and is still found in small amounts in many foods such as meat, dairy, and fish)” can alter the development of the fetus and cause problems later in life (1). The children of whom mothers’ had this chemical in their blood while pregnant are even more at risk for obesity without even having bad eating habits. Equally important are the affects it has on children's health and learning.

To begin the Cellular Respiration lab, have the lab set up as ordered on the lab manual. After reading its beginning instructions, the lab should be ready for testing the carbon dioxide concentration that is inside the chamber. Procedure should follow by weighing 10 grams of the germinating mung beans then the beans should be placed inside the Testing Chamber. Next, insert the carbon dioxide sensor with the germinating mung inside the Testing Chamber and form a closed seal. Start the machine by pressing play and collect the corresponding data. By following this procedure, calculation of the carbon dioxide concentration for dormant mung and glass beans are eligible. The variables that are controlled in this experiment are the quantities, such

During recent years, numerous newspaper and magazine articles have suggested that humans may be at risk because small amounts of well known environmental contaminants, such as dioxin, PCBs and DDT, can affect hormone levels. Hormones are produced by the endocrine system as regulators of biological function in target organs. Because hormones play a critical role in early development, toxicological effects on the endocrine system often have an impact on the reproductive system. The term endocrine disruptor is used to describe chemicals that can mimic hormones and may either enhance or counteract their effects. It has been suggested that these hormone changes can, in turn, lead to a variety of health problems

PCB’s (polychlorinated biphenyls) and dioxins have been in focus recently as there are concerns that the different structures are appearing to mimic the effects of female oestrogenic hormones. (Gray 1996) These chemicals can cause genetic chromosomal problems and major concerns to the reproductive effectiveness of marine animals. Oil spills and the burning of wood and coal can cause poly-aromatic hydrocarbons (PAHs), which has a negative effect on all wildlife causing genetic abnormalities. (EMCBTAP-ENVIS Newsletter 2002).

In 2002 a study conducted found that parabens mimicked the hormone estrogen by binding to estrogen receptors on cells. The study also discovered that parabens increase the expression of genes usually regulated by estradiol, the genes that cause human breast cancer cells to grow and