The Method Of Separation Of Charged Molecules Based On Different Migration

769 WordsJan 28, 20154 Pages
INTRODUCTION Electrophoresis is the method of separation of charged molecules based on different migration in an electric field. Applicable features of the electrophoresis technique such as high effectiveness, high resolving power, high speed, fully automation and a variety of injection based pre-concentration schemes and detection modes have all been broadly examined. (Tavares, et al., 2003). It is a developed systematic and micro preparative instrument. The method provides faster separations, at higher resolution and with greater separation efficiencies (Swerdlow & Gesteland, 1990). Capillary electrophoresis has applications in several areas like clinical/forensic, cosmetological, environmental, nutritional and pharmaceutical. (Tavares, et al., 2003). For forensic applications it has exceptional separating power, rapid analysis times, and high mass sensitivity, in terms of reagents and consumables, requiring only least quantities of sample (Tagliaro, Pascali, & Lewis, 2013) MATERIALS Instrumentation Model P/ACE 5510 from Beckman Instruments (Fullerton, CA, U.S.A.) or model HP3DCE from Agilent Technologies (Palo Alto, CA, U.S.A.) The P/ACE unit was equipped with a filter-carrousel UV detector, while the HP3DCE unit was equipped with a diode array detector. Both systems had temperature control devices, maintained at 25 - 30 °C and data achievement and treatment software supplied by the manufacturer. (Tavares, et al., 2003) Samples Clinical/forensic samples: 1. Serum
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