The Mibrobiome

The following tests according to the lab manual were performed: gram stain, fermentation tubes, methyl red, vogues proskauer, sulfur, indole, motility and growing it up on MacConkey agar. The gram stain was performed incorrectly the first time. This is because the decolorizer was not on the bacterium slide for long enough, giving a false outcome.

Three ways to assess water are positive/negative, membrane filtration and IDEXX Colilert and Enterolert assays. For membrane filtration, petri dishes and an incubator are used. For IDEXX coliert powdered medium is used for total coliform and E. Coli, while Enterolert media is used for enterococci. Also an incubator, colilert trays are used. IDEXX is more expensive than membrane filtration.

Thereafter I used a pipette to meticulously drop in water up to the 250ml mark.

Moisten filter paper with a few drops of solvent and turn on water vacuum to fullest extent

1. Fill the graduated cylinder nearly to the top with water, with a tall glass tube open at both ends (the water level with act as the closed end).

After all results have been recorded, pour all of the solutions down the drain, rinse equipment and clean up the

For the first procedure the student put 5ml of distilled water in to a test tube then picked up one filter paper and placed it in a dish. The 5ml of distilled water was then dropped on to the filter paper. The student then grabbed alcohol for its habitat solution, and placed 5 ml of alcohol in to another test tube, and placed a new filter paper in to another clean dish. The 5ml of alcohol was then dropped on to the filter paper. Once both filter papers were submersed in the proper liquid then they were taken out of the dish and out in to individual chambers that had a connection. On the left hand side was the water filter paper and on the right hand side the alcohol filter paper. The second procedure was to grab ten pill bugs and gently place them in to the alcohol and water chamber.

- Get 5 50mL beakers and fill the first one with 30 mL of water

XII. Take the 250 ml beaker to your lab bench. Set up a gravity filtration with a plastic funnel, folded wet filter paper, and an Erlenmeyer flask. Pour the content in the 250 ml beaker slowly through the filter paper. Wash the filter paper with deionized water. Dispose of the filtrate in the proper labeled waste container.

Methods: In this experiment, results were found by discovering the kinds of bacteria found in the given pond water samples. The first step in this experiment was to prepare the pond samples by completing serial dilutions of the pond water to the 10-4. We then had to prepare five separate plates by streaking the 10-4 dilutes. These plates all had different treatments. The treatments were pond 16, pond 22, pond 22 with penicillin, pond 22 with ampicillin, and pond 22 with colistin sulfate. The plates that were treated with antibiotics required 0.1 mL of the designated

This step will guide you on how to best position your aquarium, cleaning up your aquarium and placing the filter properly.

Dispense .5 mL water into the already weighed conical vial, replace cap and face insert on its down side.

Reduce heat as amount of filtrate decreases and eventually evaporates. Remove the beaker from the hot plate and allow for it to cool prior to recording its mass. Proceed to record the mass of an evaporating dish. Carefully remove the filter paper from a funnel and remove as much of the residue as possible with distilled water into the evaporating dish. Afterwards, measure 8 mL of 3M in a graduated cylinder and slowly add it to the residue in the evaporating dish.

The observed micro-organisms of watersample A were different from the micro-organism found in sample B. Sample A contained more micro-organisms with a greater diversity than sample B, which had less organisms and a smaller diversity.

Once water has been allowed to flow through this membrane, purer water can be collected from the output of the filter. Once enough of this water is collected, the hobbyist can fill the aquarium and begin to plan on his or her next step, the salt content.