The Mutant Ap Enzyme

1107 Words Nov 10th, 2016 5 Pages
Abstract: Mass sample of individuals suffer from osteopenia, which is the loss of bone density, because of hypophosphatemia. This affect is due to Falconi Syndrome. Although this is the case, some individuals with hypophosphatemia have no bone loss. The enzyme, alkaline phosphatase(AP), is proved to slow down the effects of hypophosphatemia. Few individuals with hypophosphatemia and no bone loss have found a mutated version of AP in their blood. To observe the behavior and functionality of the mutant AP enzyme versus the Normal AP enzyme, we test and compare them in different pH levels as well as different concentration levels. The first step in our experiment was determining the wave length in which the enzyme functioned the best. Our procedure found that the enzyme functioned best at a wavelength of 406.15 nm. After this we determine the optimum incubation time of the enzyme. While testing with various pH levels, we mixed together different concentrations to figure out what conditions the two different enzymes functioned coherently. Our results showed that the mutant AP enzyme functioned best in a pH of 7 while the normal AP enzyme functioned better in a pH level of 10. The goal of this experiment was to understand how a change in conditions affect the behavior of each AP enzyme.

Introduction: Hypophosphatemia is a condition where there is a lack of phosphate in the blood. This lack of phosphate results in a low bone density. According to the Journal of Bone and Mineral…
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