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The Normal Cellular Location Of The Crusoe1

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Assignment – Michael Layland – C1476301 – I1417

Q1: What does the comparison of the supernatant fraction between the experimental and control samples prove, regarding the normal cellular location of the CRUSOE1? [2 marks]
A1: Triton X-100 is a detergent in laboratory practice and is used to permeabalize unfixed eukaryotic cell membranes and aids in solubilizing membranous proteins. The addition of the substance Triton X-100 would cause the majority of the cell membranes to become permeable thus allowing contents of the eukaryotic cell to leave via simple diffusion hence the large concentration of radioactive prominence to be situated in the supernatant fraction. Again, the detergent property of Triton X-100 is further reinforced by the fact that it causes the majority of all radioactive sulphur to be situated in the supernatant. Without the addition of Triton X-100 the graph displays the greatest quantity of radioactive sulphur to be situated in fraction 3 of which a majority of “small organelles” are situated ranging from a diameter 0.2 to 1.5 μm. It is due to this fact that one can assume the normal cellular location of the CRUSOE1 protein to be situated in normal cellular location in organelles of the previously stated diameter and not it the cytoplasmic structure as the addition of Triton X-100 would assume.

Q2: Professor Holton’s team speculate that CRUSOE1 is located predominantly in the Lysosomes of the cell. Is this a reasonable assumption to make, and why? [4

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