Assignment – Michael Layland – C1476301 – I1417
Q1: What does the comparison of the supernatant fraction between the experimental and control samples prove, regarding the normal cellular location of the CRUSOE1? [2 marks]
A1: Triton X-100 is a detergent in laboratory practice and is used to permeabalize unfixed eukaryotic cell membranes and aids in solubilizing membranous proteins. The addition of the substance Triton X-100 would cause the majority of the cell membranes to become permeable thus allowing contents of the eukaryotic cell to leave via simple diffusion hence the large concentration of radioactive prominence to be situated in the supernatant fraction. Again, the detergent property of Triton X-100 is further reinforced by the fact that it causes the majority of all radioactive sulphur to be situated in the supernatant. Without the addition of Triton X-100 the graph displays the greatest quantity of radioactive sulphur to be situated in fraction 3 of which a majority of “small organelles” are situated ranging from a diameter 0.2 to 1.5 μm. It is due to this fact that one can assume the normal cellular location of the CRUSOE1 protein to be situated in normal cellular location in organelles of the previously stated diameter and not it the cytoplasmic structure as the addition of Triton X-100 would assume.
Q2: Professor Holton’s team speculate that CRUSOE1 is located predominantly in the Lysosomes of the cell. Is this a reasonable assumption to make, and why? [4
Exercise 3A is a study of mitosis. You will simulate the stages of mitosis by using chromosome models.You will use prepared slides of onion root tips to study plant mitosis and to calculate the relative duration of the phases of mitosis in the meristem of root tissue. Prepared slides of the whitefish blastula will be used to study mitosis in animal cells and to compare animal mitosis and plant mitosis.
In this experiment first the stages of an onion cell undergoing mitosis are going to be observed and every stage is going to be detected and drawn on paper. A brief description to what is going on should be attached to the pictures. This is important to understand the basics of cell division which is necessary growth,repair and asexual reproduction. Second the number of cells undergoing each phase is going to be counted to figure out in which phase the cell remains the most. If interphase is the stage in which the cell grows and prepares for cell division then the
This Lab Report is an analysis of the results of a two-part experiment. In the first part, we used a gel filtration column to separate the components of a mixture composed of protein and non-protein molecules. By doing so we hoped to obtain fractions that contained single components of the mixture, while also gaining insight into the relative molecular weight of each component compared to each other. We would then plot these fractions onto nitrocellulose paper in order to determine which fractions had protein. In the second part, we would use the fractions which we had determined had protein to conduct an SDS-PAGE. By doing so we hoped to determine an estimate on the molecular weight of the proteins present in each fraction by comparing it to a tracker dye composed of a variety of molecules of differing molecular weight.
This technique separates Rubisco samples based on their size. The electrophoresis has a positive and a negative end. Positive charge proteins are loaded from the positive end and migrate towards the negative end. Negative charge proteins are loaded from the negative end and migrate towards the positive end (Sakthivel & Palani, 2016). The sample that contained the highest molecular weight of Rubisco will travel the shortest distance on the gel while the protein with the smallest molecular weight will travel the longest distance (Sakthivel & Palani, 2016). The size proportion of each Rubisco molecule correlates with the distance traveled. Rubisco will be in its purest form after running through SDS-page since each technique will increase the purity of the protein. If the salting out, the ion exchange and the SDS-page protein isolation techniques are performed on protein Rubisco, then it is purified and separated by solubility, charge, and size. The rationale of this experiment is to isolate the purest form of Rubisco so that it can perform carbon fixation at an optimal
In this assignment I will be describing the microstructure of a typical animal cell and the functions of the main cell components. Describing and explaining the factors the ways in which materials move in and out of cells. I will also be analysing the role of the phospholipid bilayer in terms of movement of materials in and out of cells.
5.Nissl bodies are in the soma of the nucleus and are made up ofrough ER.
A.accommodation B.the concentration of cones in the fovea C.chemical structure of the vitreous humor D.the prevalence of amacrine cells in the
“Moving on to the Caitlin and the Cytoskeletons. They control our shape and movements. They gave us direct movement and keep us in one place when
Analyze the anatomical structure of ten different organelles in the cell and their respective functions.
Which of the following structures is not normally found in the cytoplasm of a resting cell?
Use complete sentences and your own words; exams will be graded manually, and reviewed by SafeAssign for plagiarism. Make sure to answer all parts of each question. Writing counts, so please plan to do a spell and grammar check. You may use any references you like, as long as the answer is in your own words and it is clear that you understand what you are saying. For most, but not all, questions you can find at least some information in your textbook.
The observation under the microscope of a cell of an onion skin soaked for 15 minutes in a 0.5 molar sucrose solution showed a cell membrane just beneath the cell wall. The cell wall had a rectangular shape. See diagram 3 for sketch. The cell and its surrounding were in an isotonic solution. The two solutions in the cell and out (0.5 molar sucrose solution) of the cell were homogenous. No net movement of water and change in the cell structure was observed. The components of the solute and solvent were evenly intermixed. The concentration of solute and solvent on either side of the cell membrane was equalized. Because the onion tissue didn’t get any water the cell was flaccid and nonturgid.
This laboratory experiment is aiming at using Drosophila as the most suitable model organism to gain an understanding on genetic concepts and principles that Gregory Mendel proposed and also to pride us with the knowledge of hypothesis testing in genetics. Materials and apparatus In order to conduct this experiment we used several materials which were provided by the lab, the materials included vial tubes, sponge covers, filter papers, labels, male and female drosophila
Hypothesis: If the salt solution concentration is increased, then the cell’s vacuole size will decrease.
Our hypothesis for this experiment was correct, the cytoplasm of the cell did shrink up because of osmosis. When we observed the onion without salt solution, the cells seemed to be full of cytoplasm and well nourished. Then, after we had filled the pipette with salt solution and added several drops to the curved section of red onion, we observed the onion with a microscope and we saw a noticeable change in the cells. The cells in the onion seemed to look shriveled up. The cytoplasm that had once filled up the cell, was almost gone with only a little amount of it remaining in the