The Physics Of Splicing And Splicing

1446 Words6 Pages
3. Conclusions Compared to the molecular methods, the optics-based method offers several advantages such as the kinetic study of splicing and splicing inhibition, study of cis–trans alternative splicing, and rapid measurement of RNA splic-ing. The kinetics of pre-mRNA splicing and the effect of isoginkgetin on the splicing kinetics of the pre-mRNA at the single molecule level were analyzed. Because of their high temporal resolution and the ability to follow the splicing of individual pre-mRNA molecules, the optics-based methods provide the evidences about the relative stabilities of weakly stabilized RNA molecules and their lifetimes. This observation attributed to the formation of mRNA molecules that are the results of the splicing of pre-mRNA. The kinetics and course of monitoring of pre-mRNA splicing was directly reached by 10 min, and are not able to be monitored by the conventional assay. The appearance of scattering intensity and SERS spectra indicates the success in monitoring RNA splicing; and the RNA splicing inhibition assay confirms that the optics-based methods can be used in study of RNA splicing inhi-bition. Moreover, the optics-based methods are able to detect alternative splicing, the RNA splicing for multi exon–intron pre-mRNA, generation of microRNA from intron of RNA processing events (e. g., maturation of microRNAs), and the siRNA maturation from the processing of host mRNAs, which are hot topics for the re-search of cancer, molecular immunology, and
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