The Process Of Sol-Gel Processing

1137 WordsNov 1, 20175 Pages
Sol-gel processing is prevented by nanomakes particles which causes pores surface with controlled pore size ranging from few nanometers to tens of nanometers to allow biomolecules loading. The dynamic light scattering (DLS) particle size analyzer (Malvern Instruments, Malvern, UK) will be used for size characterization, polydispersity index (PDI), and the zeta potential of SHMNCs. In addition, SHMNCs can be visualized by scanning electron micrograph (SEM) method (Fig. 9 B-C). The Number of pores on each SHMNC can be varied by changing the ratio of masks and interior templates molar concentration [23]. Synthesize of SHMNCs is illustrated in (Fig. 9): 1. Mixing of positively charged amine-functionalized polystyrene beads (available at…show more content…
Hence, different size of SHMNCs need to be fabricated (Table 2). The pores size on the surface of SHMNCs are controllable by choosing different nanomasks’ diameter. The size of each pore will be approximately around 25-35% of nanomask diameter. For instance, using 100, 60 and 40 nm nanomasks diameter, result mesoporous of 30±4, 20±3 and 12±2 nm in diameter, respectively [23]. The pore size should not be smaller than the maximum length of enzyme which needs to be loaded in SHMNCs. Here, since the maximum size of enzyme is 12.2 nm (CaMKII), the pores size cannot be less than 12 nm. Therefore, the 40 nm nanomask is the smallest nanomask that will be applied to create SHMNCs. Table 2: Various sizes of SHMNCs with different pore sizes and numbers SHMNC name Interior template size Nanomask size # Pores SHMNC 500/100/25 500 nm 100 nm 25 SHMNC 500/100/15 500 nm 100 nm 15 SHMNC 500/60/25 500 nm 60 nm 25 SHMNC 500/60/15 500 nm 60 nm 15 SHMNC 200/60/25 200 nm 60 nm 20 SHMNC 200/60/10 200 nm 60 nm 10 SHMNC 100/40/10 100 nm 40 nm 10 SHMNC 100/40/5 100 nm 40 nm 5 After SHMNC sol-gel processing followed by calcination, due to partial dehydration of silica gel hydroxyl group, the final diameter of SHMNCs will be around 85-90% of templates size [60]. Also, particle size distribution will be obtained by high-resolution technics such as Atomic Force Microscopy (AFM), or Dynamic Light Scattering (DLS) methods. It is
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