The Production of Pyruvate and Acetaldehyde During the Fermentation of Glucose.

This lab was to investigate fermentation, a cellular process that transfers the energy in glucose bonds to ATP. The energy in ATP can then be used to perform cellular work.

This process does not require oxygen and is referred to as fermentation. This process partially breaks down carbohydrates and it obtains a small amount of energy, again in the form of ATP. Pyruvic acid has to be broken down in respiration when formed by breaking down of glucose molecules, this can't be done in the same way as in aerobic respiration. When anaerobic respiration is taking place carbon dioxide and ethanol is formed.

The purpose of this investigation is to test the effect of different sugar sources on yeast respiration.

Respiration is a chemical process by which organic compounds release energy. There are two types of respiration reactions that cells use to provide themselves with energy: aerobic and anaerobic (fermentation). (Chemistry for Biologists: Respiration. 2015) Both processes are similar within the initial steps of the reaction- beginning with glycolysis. However, in fermentation instead of the pyruvic acid being converted to acetyl coenzyme A, it’s converted into both ethanol and carbon dioxide in yeast and some plants and lactic acid in animal cells. Another distinct difference between the two processes is that anaerobic respiration uses oxygen

For the cell to generate adenosine triphosphate (ATP), plenty of oxygen is needed. Fermentation is a unique way to generate energy ATP in the form of without using any oxygen. Fermentation consists of glycolysis and a process of NAD+ generation. Glycolysis oxidizes glucose to two pyruvate molecules. The two pyruvate molecules are converted into two carbon ethanol molecules, and two carbon dioxide molecules are released. The overall net yield of fermentation are two ATP molecules and two NADH

One of the most significant reactions in Glycolysis is reaction one which involves the phosphorylation of glucose to form glucose-6-phosphate. Through the transfer of the hydrolysis of ATP, this supplies energy for the reaction and makes it essentially irreversible, having a negative free energy change, which allows for a spontaneous reaction in cells. Although the preparatory phase is energy consuming and uses up 2 ATP, the pay off phase synthesizes 4 molecules of ATP, with the transfer of 4e- via 2 hydride ions to 2 molecules of NAD+. Therefore, a net gain of 2 ATP is achieved through the glycolytic pathway alone. Following the glycolytic pathway, due to the absence of oxygen, as oxygen cannot be supplied fast enough to undergo aerobic respiration, the athlete will instead, undergo lactic acid fermentation. Lactic acid fermentation involves pyruvate that is formed from the glycolytic pathway to be reduced to lactate, with the aid of the enzyme, lactate dehydrogenase, while the coenzyme Nicotinamide Adenine Dinucleotide (NADH) is oxidised to NAD+. The product NAD+ then re-enters the glycolytic pathway in order to produce 2 ATP. This process of lactic acid fermentation produces 2 ATP for each cycle, and thus, rapidly supplies the body with a small amount of energy. However, with the buildup of lactic acid in the body, the athlete will eventually encounter the feeling of discomfort as this accumulation of lactate causes the body to

Cellular respiration is the series of metabolic process by which living cells produce energy through the oxidation of organic substances. Cellular respiration takes place in the mitochondria. Fermentation is the process by which complex organic compounds such as glucose, are broken down by the action of enzymes into simpler compounds without the use of oxygen. The significance of these pathways for organisms is to allow for an organism to be able to generate ATP. Some organism that undergo cellular respiration are bacteria and fungi. Some organism that undergo fermentation are yeast and muscle cells. In cellular respiration, glucose is oxidized and releases energy. In cellular respiration, glucose produces ATP and 3-carbon molecules of pyruvate. The pyruvate is then further broken down in the mitochondria where it becomes oxidized and releases CO2 (Upadhyaya 2014). In the fermentation process oxygen does not play a part. This process converts glucose into pyruvate and produces ATP. From there pyruvate breaks down into CO2 and acetaldehyde (Upadhyaya 2014) Monosaccharides are known as simple sugars and their main function is being the source of energy for organisms. Disaccharides are two monosaccharides joined by a covalent bond and their primary function is to provide food to monosaccharides. Some disaccharides

A). The anaerobic metabolism of glucose to pyruvate is called glycolysis. This sequence of reactions will generate two molecules of pyruvate for every one molecule of glucose. This metabolism is anaerobic, which means that it does not require oxygen to be completed. The first phase of the process of glycolysis is called the preparatory phase. The entire process of glycolysis is started once glucose is trapped inside

In this lab we tried to find what fuels yeast could metabolize and what the yields of the carbon dioxide gas that were produced from the different sugars used. We used 6 different yeast and sugar mixtures. The different yeast and sugar mixtures we used were control, glucose, sucrose, fructose, starch, and saccharin. The results for the 6 different results are presented in Tables 1-6 and Graph 1. Graph 1 is a graph of all the information in Tables 1-6. Each Table and graph is labeled approximately.

This lab investigates the effects of Sucrose concentration on cell respiration in yeast. Yeast produces ethyl alcohol and CO2 as a byproduct of anaerobic cellular respiration, so we measured the rate of cellular respiration by the amount of CO2

Glycolysis is followed by the Krebs cycle, however, this stage does require oxygen and takes place in the mitochondria. During the Krebs cycle, pyuvic acid is broken down into carbon dioxide in a series of energy-extracting reactions. This begins when pyruvic acid produced by glycolysis enters the mitochondria. As the cycle continues, citric acid is broken down into a 4-carbon molecule and more carbon dioxide is released. Then, high-energy electrons are passed to electron carriers and taken to the electron transport chain. All this produces 2 ATP, 6 NADH, 2 FADH, and 4 CO2 molecules.

Uniquely, glycolysis is both anaerobic and aerobic. The end product pyruvate, from glycolysis, is anabolized to lactic acid when there is a need for energy without an adequate supply of oxygen available. This last step or reaction enables glycolysis to continue producing ATP without the need for oxygen, which is why it is called the anaerobic energy system (Fink, 2009).

Sugars are vital to all living organisms. The eukaryotic fungi, yeast, have the ability to use some, but not all sugars as a food source by metabolizing sugar in two ways, aerobically, with the aid of oxygen, or anaerobically, without oxygen. The decomposition reaction that takes place when yeast breaks down the hydrocarbon molecules is called cell respiration. As the aerobic respiration breaks down glucose to form viable ATP, oxygen gas is consumed and carbon dioxide is produced. This lab focuses on studying the rate of cellular respiration of saccharomyces cerevisiae, baker’s yeast, in an aerobic environment with glucose, sucrose, lactose, artificial sweetener, and water as a negative control. A CO2

The first part of the breakdown of glucose is known as Glycolysis, in which one molecule of glucose is broken down to two molecules of pyruvate (C3H4O3). The second part is the fermentation process in which the two pyruvate molecules are converted to two molecules of ethanol and two molecules of carbon dioxide.

pyruvate (3 carbon sugar), 2 NADH and 4 ATP (2 net) per molecule of glucose. During