The Purpose Of Pglo Transformation

Genetic engineering (GE) is a recently developed technology that allows the alteration of the genetic make up of living organisms. This technology allows scientists not only to exchange genes from members of the same species, which is what farmers and nature has been doing through out history, but also the exchange of genes between completely separate species. For example genetic engendering allows scientists to insert the genes from a fish into a tomato, something that can never happen in nature. Many in the biotechnology industry claim that the recent advancements in this field of research will help solve many of the problems developing countries face. These claims have led to a massive increase in commercial growing of genetically

The pGLO plasmid will transform the E. coli bacteria with a gene called GFP that codes for the Green Fluorescent Protein in the genetic code. GFP was discovered in the jellyfish, Aequorea victoria as a green fluorescent light emitted from the jellyfish. It was typically seen in the dark upon its activation and since then has been used in studies relating to genetic transformation. (Chalfie and Tu 1994) The majority of the studies test the many different factors that are required in the transformation of pGLO which will determine the functionality of GFP in the E.coli bacterium. The first experiment in transforming GFP and E. coli was completed in 1994 by Chalfie and was further refined the same year. The experiment proved the importance of using restriction enzymes, and DNA ligase in the process of transforming GFP to identify arabinose as the primary activator, and to identify the ampicillin

Genetic Engineering is the direct modification of an organism's genes by using biotechnology. Scientists can genetically modify an organism’s chromosomal makeup by inserting proteins that will

First of all, a definition of genetic engineering is in order. Genetic Engineering is defined by Merriam-Webster as "the science of making changes to the genes of a plant or animal to produce a desired result" (Genetic Engineering, n.d., para. 1). At first glance, genetic engineering sounds remarkable similar to cloning, which is also a hotly debated bioethical issue that has recently arisen. Cloning, however, differences from genetic engineering in the end result. While genetic engineering allows for pinpointing and transferring a specific gene known to produce specific trait in an organism, cloning is making an exact copy of the organism 's DNA (Holtzmann, 2014). It 's important to note that both cloning and genetic engineering, in their most rudimentary forms, have been practiced for years. As science has progressed over the years, it has become better at and found new ways of injecting new traits into plants to make them less susceptible to insects in an effort to prevent the erroneous use of pesticides, or to produce larger yields with larger plants. The end result of these technological innovations: known as genetically modified organisms (GMOs) have started a worldwide debate as to the environmental

Transformation is any change in an organism that alters its general character. It means “change caused by gene.” (biology-online.org) Genetic engineering is the process of manually adding new DNA to an organism. The main goal of genetic engineering is to add one or more new traits that the organism does not already have. ( ) There are many benefits of genetic engineering. With genetic engineering many illnesses and diseases can easily be prevented by isolating the gene that causes them. Also, genetic engineering has the ability to increase genetic diversity and pinpoint desirable traits of certain organisms. There are also various disadvantages of genetic engineering. For example, the process of genetic engineering is a long process that is risky and tricky. You need a variety of information in order to attempt the process. There is also debate about genetic engineering. Some people believe that it messes with the moral issues in religion.( )

Genetic engineering is the alteration of an organism’s genotype using recombinant DNA technology to modify an organism’s DNA to achieve desirable traits. (OpenStax 459). The main purpose however of genetic engineering is to deepen the understanding of how genetics works, as well as how it can be applied to the rest of the world. For example growing body parts on mice, or make crops resistant to pesticides, help them grow faster, make them produce faster and much more. The process of genetic engineering according to FAO Corporate Document Repository occurs in 9 steps:

Genetic engineering is the process of changing an organism's genetic material. Genetic engineering is also used in the processes of heredity reproduction (genetic engineering, 1). Genetic engineering alters the genes of an organism to give it desired characteristics, these techniques have been used in plants, animals, and even people. Researchers are working on curing genetic diseases by genetic engineering. Genetic engineering techniques include a wide range of procedures that alter the reproductive and hereditary processes of organisms. Depending on the problem, the procedures

Geneticists continue to look for ways to genetically alter genes and transformation to better the lives of other people. In this experiment the goal was to understand the transformation process and how genetically engineering bacteria could possibly be useful. The transformation process involved 5 steps: pre-incubation, incubation, heat chock, recovery and growth, and selection. Four different plates were used to analyze the effects of the transformation. The plates were as follows: plate 1 contained lysogenic broth (LB) and ampicillin (amp) with plasmids (pGLO); plate 2 contained LB/amp/arabinose sugar with pGLO; plate 3 contained LB/amp without pGLO; and plate 4 contained LB without pGLO. The results obtained proved our hypotheses to be correct.

Genetic engineering techniques have been applied to various industries, with some success. Medicines such as insulin and human growth hormone are now produced in bacteria, experimental mice such as the oncomouse and the knockout mouse are being used for research purposes and insect resistant and/or herbicide tolerant crops have been commercialized. Plants that contain drugs and vaccines, animals with beneficial proteins in their milk and stress tolerant crops are currently being developed

Genetic engineering is the scientific process of altering the genes in an organism's DNA resulting in a desirable outcome. Genetic engineering was first heard of in 1941 but, did not take off running until the 1970s. Everything started with genetically altering plants DNA such as, Bt corn, rice, wheat, and roundup ready soybeans; “products that have been the focus of controversy and protest around the world” ( Fukuyama 671). Not only did scientist alter plants but, took it a step further and started genetically altering animals that resulted in wanting for more desirable traits in the animals. Once scientist realized that it was possible to alter the DNA sequence of plants and animals, then their focus turn to the possibility of altering humans.

Steven C. Austin 11/6/17 LIFE 102 – L34 The introduction of a green fluorescent protein into the DNA of E. coli using the heat shock method. Introduction: The purpose of this experiment is more significant than meets the eye. The true nature of this experiment was to find competent cells that were able to take in foreign DNA, but the importance of genetic transformation translates to all forms of life.

Industrial applications include transforming microorganisms like bacteria or yeast, or insect cells with genes for creating a useful protein.

For those who are unaware, genetic engineering is the alteration of a single DNA cell in order to manipulate a living organism to “resurrect” or revamp a species. Biotechnology allows geneticist’s to edit DNA, insert it into an

Genetic Engineering is a process where we manually remove our gene of interest from an organism and insert it into another organism. This results the organism to possess the particular trait we want from the organism. Applications of genetic engineering can be found all around us. They can range from certain crops that are pest resistant to the food with higher nutritional value and even to the medicine that we consume. Genetic engineering began in 1973 when Herb Boyer and Stanley Cohen created the first recombinant DNA organism. They transferred antibiotic resistance genes into a plasmid. The plasmid was later introduced into Escherichia Coli. They then discovered that the following generations of daughter cells were also resistant to the

Genetically Modified Organism (GMO) is one of the most important issues around the world. Genetic engineering is a process where scientists take genes from one species and force it into the DNA of other species. GMO has long been in practice to breed select individuals of a species to produce offspring of the desirable behaviors. It is used in conventional livestock production, crop farming and even pet breeding. It involves combining elements of DNA from different sources to create a new DNA molecule that has a different combination of genes than original, which occurs naturally. There are some scientific methods for producing GMOs, such as recombinant DNA technology and reproductive cloning. Recombinant DNA technology involves the