The Roundup Ready Solution Is An Important Legume Crop
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Introduction The Glycine max (L.) Merr soybean is an important legume crop (Zhang et. al. 2013). Soybeans are used to produce oils, proteins (Zhang et. al., 2013), and soymeal (Cherubin, 2008). Soybean oil is largely produced and consumed globally (Cherubin, 2008). Soymeal provides 70% of an animal’s protein (Cherubin, 2008). Canada is a top producer of soybeans worldwide (Cherubin, 2008). The Roundup Ready (R.R.) gene is often incorporated into the soybean seeds (Villela et. al., 2014) and is sold by licensed seed companies such as Monsanto, Pioneer, and Novartis (Bullock et. al., 2004). The Roundup Ready solution is a glyphosate compound, which is a low cost yet effective herbicide (Bullock et. al., 2004). The R.R. gene is incorporated…show more content… The objective of this study was to determine the level of the Roundup Ready gene in a sample of soybean plants and determine the efficacy of an Agdia strip test as measured by the frequency of false positives and false negatives of the test in comparison to the reaction of plants to the herbicide glyphosate.
Methods and Materials In this experiment, Glycine max (L.) Merr soybeans were used. 40 of these soybeans were provided for the experiment. Each student was assigned a specific soybean plant to use. A small leaf would be torn from the stem of the soybean. This leaf was then weighed using a top loading balance. The leaf was to weigh approximately 0.12 to 0.16 grams. Gloves were worn when handling the extraction bag. The top of the bag was cut using scissors, and the leaf was then placed between two layers of mesh in the bag. The bag was then temporarily sealed to do the extraction. Students used the blunt end of a pen to rub the leaf in order to mix the contents. The extraction then sat for 30 seconds minimum, then an Agdia ImmunoStrip was provided to test the solution. The ImmunoStrip was inserted into the contents by no more than 0.5 centimeters. The ImmunoStrip was held still in the bag for 3 to 5 minutes, or until the ImmunoStrip appeared complete. A single test stripe will appear first to indicate that the ImmunoStrip is working. A second control stripe