The Separation Of A Solvent

5. Prepare the salt solution by mixing 1/8 teaspoon of salt and three cups of water in a clean pitcher or 2-liter bottle. Stir or shake the solution until it is dissolved. This will produce a 1% salt solution.

At the end of the 75 minutes, two 1.0 mL samples of the albumin/glucose solution from the beaker were added to two test tubes labeled solution end. Then, the dialysis tube was removed from the beaker and rinsed off with distilled water. Once the tubing was rinsed and blotted dry the final water weight was recorded. After measuring the final water weight, the contents in the tubing was dumped into a beaker and 1.0 mL of starch/sodium sulfate solution was added to two test tubes labeled bag end (Keith et al., 2010).

1. Cut four 6-inch pieces of dialysis tubing and soak in a coffee cup filled with tap water for 2 hours prior to your start

The dialysis tubing will be clamped at one end in order to fill it and then clamped at the other end to seal the filled bag. If the bag is not soft and floppy, the experiment will not work. Blot a bag with a paper towel to absorb the moisture and weigh it, if this blotting process is not done it could interfere with the weight readings creating inaccurate information. After the bags of the solutions are prepared, they will be placed into five different beakers with different solutions. Beakers 1-4 will be filled with tap water and the fifth beaker is filled with 40% sucrose and water. Fill each beaker with just enough water or solution so that the bag is covered and place the bags in the beakers simultaneously and record each time. Every 10 min the bags are to be taken out, blotted, and weighed again before returning them back into their respective beaker for another 10 min. The process is repeated until you have reached 90 min. The weights should be recorded in grams (g).

Then added 5.00g of CuCl₂ ●H₂O into the distilled water. Stirred to dissolve the mixture

Using the graduated cylinder, measure 20mLs of the stock sucrose solution and 180mL of water to create a 3% sucrose solution and place it into the 250mL beaker (beaker #2). Place bags #1‐3 (red, blue, yellow) into beaker 2 and bag #4 (green) into beaker 1. Allow the bags to sit for one hour. After allowing the bags to sit for one hour, remove them from the beakers carefully open the bags, noting that often times the tops may need to be cut as they tend to dry out. Measure the solution volumes of each dialysis bag using the empty 250 ml beaker.

Measure 500ml of tap water in the 500cm3 beaker, then measure 5g of sodium hydrogen carbonate using the 50cm3 beaker and weight scale and place in the beaker of water, using the glass rod to dissolve it into the mixture.

Prepare standard solution #1, Take 1 ml sub stock solution from the 100 ml beaker and then put into 25 ml volumetric flask with the help of 10ml graduate pipette.

Weigh the nut, the aluminum cup, and the test tube clamp all together. Record the total

Create a water bath by filling ½ of the 100 mL beaker with cool water, adding crushed ice to the beaker so the water level is just below the top, and sprinkling salt into the beaker

4.Measure 35mL of warm water and add them into each of the 4 test tubes at about roughly the same time. It is essential that the water is warm. Do not seal the test tube.

XII. Take the 250 ml beaker to your lab bench. Set up a gravity filtration with a plastic funnel, folded wet filter paper, and an Erlenmeyer flask. Pour the content in the 250 ml beaker slowly through the filter paper. Wash the filter paper with deionized water. Dispose of the filtrate in the proper labeled waste container.

1) Pour 25 mL of the 1 M hydrochloric acid into the beaker and rinse the solid by swirling the acid around in the bottom of the beaker.

Record the sample mass in your notebook. Remember to record all digits on the balance. Closed toed shoes and goggles are REQUIRED for the lab

2. Allow the beaker to cool until it reaches room temperature; then weigh the beaker with the contained solid. The loss in mass represents the amount of NH4Cl in your