The Specificity of Albumin Binding Experiment

1301 Words May 5th, 2013 6 Pages
The specificity of albumin binding experiment was to determine the binding interactions that occur between serum albumin and three synthetic dyes with the use of electrophoretic procedure. Whole blood, or plasma. Clots upon standing and if the clot is removed, the remaining straw colored fluid is called serum. The major protein in serum is albumin which functions as a carrier molecule for the transport of certain small molecular weight compounds in blood. Molecules that bind to serum albumin are fatty acids, hormones and some synthetic dyes. In this experiment the synthetic dyes used are Bromophenol Blue, Ponceau S and Orange G. we observed that free dyes not bound to albumin migrate faster that albumin or dyes bound to albumin. This …show more content…
The 2nd half for the gel is our experiment compared to Anderson group 6. The 1st layer in all 3 pictures is the bound dye, the 2nd layer is the free dye.
Fig1 shows on the right group one next to it, 1st half of our result on electrophroresis gel. The 2nd half for the gel is our experiment compared to Anderson group 6. The 1st layer in all 3 pictures is the bound dye, the 2nd layer is the free dye.

Group 1, the gel picture and Andersons experiment are closely related. The binding did occur, in the 1st lane of group 1the dye was free therefore no binding occurred because no Bovine Serum Albumin (BSA) was present in the mixture. While in lanes 2 to 4 binding did occur because there was BSA in the mixture, with the higest binding occurring in lane 4, this is because there was no buffer to interrupt the binding. (saturation).
Group 6, which is the 2nd half of the gel picture, does not correspond with Anderson results, no visible binding occurred. This group had two different dyes and SDS included in the mixture also. In Andersons version although two different dyes were used, it didn’t affect his results, it’s the SDS that…

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