The Staining Of Bacteria Are Known As Differential Technique Of Staining

Proper collection of blood cultures are necessary and the most direct method of determining whether or not a patient is septic. The purpose of obtaining blood cultures is to identify and isolate the bacteria that are causing an illness and then determine the best course of treatment based on the sensitivity of the bacteria to particular antibiotics. One of the most frustrating problems plaguing hospitals is the increased rate at which blood culture results are being returned as contaminated specimens. These results can lead to a significant increase in cost to the hospital and patient as well as an increased length in hospital

This experiment was conducted to find the genus and species of an unknown bacteria prescribed by the lab teacher, which was unknown bacteria GA3 in my case. Identification of unknown bacteria techniques are used on an every day basis to figure out what type of bacteria it is and to find the best method of how to treat a patient with this bacteria (1). All five “I’s” of Microbiology were used in the testing for the unknown culture. Inoculation was used several times to put the unknown culture into agar plates or into biochemical test tubes. After Inoculation of these tubes or plates, they always were placed into the incubator for further growth and development. Isolation was used to make sure we got the correct bacteria we were testing for. After each further isolation, we gram stained the culture and inspected the culture under a microscope to further help in the identification process of the unknown bacteria. Multiple tests were done on the unknown culture to make sure we were confident in what kind of bacteria the unknown was.

Preliminary studies help identify Genus species of bacteria. Two different preliminary study pathways must be used since two different pathogens were found in the sample. A dilution and a quadrant streak are the ideal methods to separate pure cultures of bacteria. MacConkey Agar and CAN (MAC) is a selective media that is used for the cultivation of gram negative bacteria. (PEA) is a selective media that is used

The purpose of the following study is to determine where the two unknown bacteria acquired in Microbiology lab should be classified in regards to temperature, pH level, and osmoregularity. It is important to classify bacteria in order to identify them. Identification of bacteria is important because they are not only useful but potentially dangerous as well. The identification of bacteria can lead to breakthroughs in healthcare regarding treatment of old and new diseases alike. Identifying bacteria can also be used in many other areas from better crop production through microbial pesticides to biological warfare. Their uses are endless as are their abilities to evolve and adapt to changing environments. That is why it is so important

In this experiment, an unknown bacterium was given to each individual student. The main purpose of this lab was to identify the given unknown bacteria going through a series of biochemical tests as one of the gram negative bacteria among six different Gram negative bacteria Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, Proteus mirabilis, Pseudomonas aeruginosa and Salmonella typhimurium. At the very beginning, streaking method; T-streak technique was used to isolate the pure colonies. For the morphological identification of unknown bacteria, Gram Stain Method was done. Biochemical tests that were conducted for the experiment

As the flowchart shows, a series of tests were conducted to identify the unknown bacterium #65. Microscopic observation of the gram stain indicated a gram-positive coccus bacterium. S. epidermidis was used as the gram-positive control while E. coli was used as the gram-negative control. This observation led to the elimination of all gram negative and rod-shaped genera: Enterobacter, Citrobacter, Klebsiella, Escherichia, Pseudomonas, Serratia, Alcaligenes, Neisseria, Proteus, Salmonella, Shigella, Erwinia, Veillonella, Flavobacterium, Bacillus, Arthrobacter, Lactobacillus, Listeria and Kurthia (2). By performing the catalase test, it was determined that the bacterium was catalase negative and it did not produce bubbles. M. luteus and E. faecalis were used as positive and negative controls, respectively.

There are many reasons for identifying an unknown bacterium. The purpose of this exercise was to identify an unknown bacterium from a liquid culture. We chose our unknown bacteria from a rack of test tubes with several different species of bacteria inside. I wanted to pick an unknown bacteria with a number easy to remember so I pick the test tube labeled “745”. Procedures were followed as stated in the lab manual written by Dr. Pedro J.A. Gutierrez.

The purpose of this lab was to identify an unknown microorganism using lab techniques. The importance of identifying microorganisms is essential to the survival of humans, expansion of modern day medicine and improvement of quality of life. In 1884, Hans Christian Gram designed a differential staining technique to identify bacteria that would change the future of microbiology. He give rise to a staining process, known as the Gram stain to differentiate microorganisms into two groups between positive and negative gram staining microorganisms. The Gram stain is essential in a lab technique as it distinguishes the cells based on the physical properties of the individual cell walls, and is almost always the first test preformed to differentiate a microorganism. The identification of weather a microorganism is gram positive or negative can revel the bacteria’s virulence, cell wall structure, resistance to antibiotics, resistance to physical disruptions and so much more. In order to identify the unknown provided, unknown #27, the Gram stain was the first test preformed. After discovering that the unknown bacterium was indefinitely a gram positive microorganism, the vast possibilities were narrowed down. However, In order to more definitively identify the unknown, the next step was to preform biochemical tests. A biochemical test identifies metabolic

The purpose of the Unknown Project is to help microbiology students learn to identify an unknown bacterium in a mixed culture by performing different types of tests in a lab. The student must be familiar with the tests and should be able to analyze and interpret the results. Based on the results attained, an organism may be proposed. But what is a mixed culture? A mixed culture contains either two or more different types of identified microorganisms in a container, like a test tube for example. The culture may carry either organisms from the same bacterial family or it may contain a mixture of fungi and bacteria or other combinations. Being able to identify different types of organisms is essential for us to know if by any case, risks are presented. These cultures are performed mainly in hospitals. They are obtained from different parts of a patient’s body, depending on the suspected infection. Registered Nurses can perform certain cultures like blood, urine, sputum, etc. and send them to the lab. A mixed culture has been helpful in previous medical research by helping determine whether a patient has a bacterial infection or not. For example, “Sputum samples often give mixed cultures. Since the patient coughs up the sputum from the lungs, the sample can become contaminated by normal mouth bacteria, resulting in multiple types of bacteria in the culture.” (http://www.ask.com/science/mixed-culture-microbiology-af200d033ce00db0) Many tests may be performed on this mixed

An unknown bacterium 15 was awarded and labeled at the table ready to be identified. Using the skills and test that are taught and learned in microbiology were applied into learning what the unknown bacteria culture was. There were multiple procedures and test done in order to gain all the information needed to determine which bacteria was given. In order to find what the bacteria was the first step was finding the right environment and temperature that would allow the bacteria to thrive and grow. Determining this is one of the most important steps in being able to obtain conclusive results that would allow the results of the test to be accurate and correct. Without the correct temperature and environment the bacteria will give inconclusive results which will alter and skew the end results and may lead to the wrong conclusion. By using the methods that were obtained and learned through the microbiology class allowed the skills and knowledge to determine the bacteria and execute the tests in order to determine the culture.

In the world of microbiology it is vitally important to be able to discern the identities of microorganisms. Not only is it important in a lab setting but as well as in healthcare in general. Properly identify what strain of bacteria a person has will aid in the proper medicine and dose given. Throughout the semester we have learned about different types of bacteria and certain test that can clearly identify them. The purpose of this lab report is to identify a Gram-positive or Gram-negative bacterium. Using all the knowledge of procedures and lab techniques identify the unknown and discuss all the tests you performed.

For this experiment, I utilized unknown number three which I later identified as Staphylococcus epidermis. I concluded that the unknown organism was Staphylococcus epidermis based on numerous tests performed in the laboratory which I will discuss in detail throughout this paper. One of the first tests performed was the Gram Stain. The Gram Stain

There are many reasons for knowing the identity of different microbes when presented with them. The reasons include identifying the causative agent behind the disease in a patient, understanding how it can be treated, as well as knowing the microbe that should be used for making the right antibiotics. With this being said, their correct identification is not only significant in a microbiology lab but also in the medical, manufacturing, and pharmaceutical fields. The first test used in the identification of unknown bacteria numbers 3 and 4 was the Gram stain, which was used to differentiate between gram-positive and gram-negative bacteria based on their different cell wall elements. The Gram stain procedure distinguishes between

There are many different physiological tests one can used to identify bacteria. For this project, one can use a spore stain to see if a bacteria can produce spores in response to nutrient depletion.(Intrieri, 2014) There is also the catalase tests, which show whether bacteria possesses the enzyme catalase, which can handle reactive oxygen species (Intrieri, 2014). There is also the oxidase test, which tests for the presence of oxidase in a bacteria, which indicates the bacteria contains cytochrome c (Intrieri, 2014). Another useful test to differentiate bacteria is the Entero-Plueri Test®, which performs multiple tests in one setting, like

There are two major classes of bacteria that contain peptidoglycan, Gram-positive and Gram- negative. Gram positive bacteria will stain dark purple due to their thick peptidoglycan cell wall, while Gram negative will stain pink due to their thin peptidoglycan cell wall. Many Gram positive and negative bacteria are considered pathogens because they are capable of causing disease in humans. In order to treat properly individuals who are infected with a pathogenic Gram positive or Gram negative bacteria, the identity of the bacteria must be found. In this experiment numerous, differential and selective media, as well as chemical test, were used to evaluate the growth patterns and characteristics of clinically relevant bacteria. Data collected from the specific results was used to create a chart that was used to identify an unknown Gram-positive and Gram negative bacteria.