The Surface Bacteria Investigation And Antibacterial Growth Of E.coli

9. After the 48 hour time period the inhibition of the Escherichia coli bacteria around the disinfectant disks was measured. A ruler was used to measure the clearing from the edge of the disk to the perimeter of the clearing. Irregularities in the clearing were not included in the area of clearing.

“Ajax Spray ‘n’ Wipe” specifically states that it kills E. coli. This is because of the lactic acid in the spray, which is used as a natural antibacterial agent.” ("Colgate-Palmolive | Product Ingredients"). Lactic acid kills unwanted bacteria especially in food which is where E. coli is often found and transmitted, of which meat is the most common cause. Vinegar was chosen as a substance because “it can be used as a bleach alternative” (“Disinfectants: A Guide to Killing Germs the Right Way”) and bleach kills E. coli. It is also used as an everyday cleaning product. The last substance chosen was salty water because it is thought that salt kills bacteria. Much of the world’s population has access to salt and if the experiment proves that salt kills E. coli it would be a helpful factor for further treatment of the

On August 19th, 2015 this experiment was performed, by 6 separate lab groups.The experiment began by measuring 1 Ml of E. coli into a pipette and pump, then placing the bacteria into a culture medium. The E. coli and medium were then swirled together for a period of 15 minutes, until completely mixed. This mixture was then poured into a petri dish and allowed to solidify for 45 minutes. After the 45 minute solidification time, 5 small paper disks were inserted into the dish. 4 of the disk contained treatments of antibiotic and 1 was left untreated. The

5) My hypothesis: “The higher the concentration of antiseptic, the more powerful it will be at inhibiting/ killing microorganisms which cause bacterial growth.” This theory supports my result because as you can see the zone of inhibition increases due to the increasing concentration of antiseptic. The higher concentration of antiseptic means it has been less diluted, which means its more stronger than other more diluted concentrations, this high concentration will determine how powerful the antiseptic will be at killing/inhibiting the growth of bacterium, this high concentration of antiseptic will break down the bacterial cell wall, after this process has happened the antiseptic can stop the mutation of the bacteria and inhibit the

You can grow microorganisms in liquid media or on solid media. Most bacteria can be grown in labs as long as the media contain a source of the major nutrients; carbon, nitrogen, sulphur, and phosphorous. They may also need to have other nutrients. These nutrients are made into a broth and the pH and salinity can be adjusted. The nutrient broth is placed in test tubes, which are plugged with cotton wool, capped with foil and then sterilised in an autoclave, at 121c for 20minutes. These tubes are then cooled before they are inoculated. To prepare the streak plates we dipped an inoculating loop into ethanol then placed it in the flame until the loop glowed red. Still holding the inoculating loop by its handle we removed the lid from

Some brainstorming was done to determine the lineup of the bacteria on two Petri dishes. Next, the experiment started with the labeling of the two Petri dishes. This was followed by a mistake that occurred, which was corrected with an orange dry-erase marker. Then our hands were sanitized with hand sanitizer, and was followed with bacteria collecting. The bacteria was collected, from the silver-ish, doorknob on the inside of the door near room B216 (with evacuation plan), and the beginning part of the handrail at the right found near the stairs of the B building opposite to Luck Hall. After that, the process was

Testing the Effects of Bacteria Growth on a Non-Treated Vs. Treated Hand Name: Laura Torres Biology 1407-P Abstract The objective of this experiment was to test the effects of bacteria using a treated hand and an unwashed hand. Two plates of agar and a hand is used to retrieve the bacteria and then placed in a temperature where they can survive. Different types of bacteria are affected by the environment that slow down or increase the growth of these prokaryotes.

In this experiment, pH indicators, colour indicators, metallic ions, and Kovac’s reagent all aide in the differentiation of different bacteria under different conditions. Proteus vulgaris for example is a rapid production of urease and this is shown through the phenol red indicator, turning from yellow to pink. This experiment is usually done to distinguish from Proteus and other bacteria, which helps to isolate these bacteria since they are infectious (O'hara, et al., 2000).

In this experiment, I hypothesized the women’s restroom soap dispenser will contain more microorganisms, rather than the student’s hallway bench. The Microorganisms are very tiny organisms that travels through the cell caused by archaea and protists bacteria that appears in the human body. For example, Microbes appears thorough our skin, mouth and into the air. It is not visible for you to touch, feel, or see. These tiny organisms can contribute to worse conditions. The worse conditions cab be spread, directly or indirectly to certain cancers, bowel syndrome, asthma, and autism (Campbell 2015). Also, microbes changes can effect on how we eat, live, and move around in the human body. Microorganisms reflect the changes of the environment

Bacillus causes anthrax; it is one of the most deadly bio weapons. Anthrax is classified as a class A endangerment according to the CDC. It poses a significant risk to nation security. The gram positive, rod shaped anthrax spores are naturally in soil. It can be produced in a lab; it lasts long in the environment. Bacillus has been a biological weapon for over a century; it’s been mixed before with powders, sprays, food, and water. It is invisible, infectious, odorless, and tasteless. Spores make anthrax a very flexible bio weapon. Letters were once sent out from the US postal system in 2001. It affected a total of 22 people in which 5 died.

Lee and Bishop (2016) have stated, microbes are microscopic, living single-celled organisms such as bacteria, fungi and virus which is non-living. The structure of bacteria that causes disease are the fimbriae, flagella and toxins. The cell wall provides structural support and the cell membrane supports the functions that occur in the subcellular structure of higher organisms. Bacteria are also known as pathogens which cause diseases in plants, insects, animals and humans. Flagella are the long tail-like structures that enable the bacteria to swim or move and it also help the bacteria to migrate to its site of infection and survive. Toxins are very harmful which are responsible for the symptoms and complication of the disease. One of the chronic disease caused by bacteria is tuberculosis (TB). A favourable environment such as time, temperature and oxygen are important to facilitate the bacterial growth.

Bacteria are single-cell microorganisms that cannot be seen by the naked human eye. Bacteria are in many different classifications. Anaerobic bacteria, also known as anaerobes, are bacteria that do not survive with the assistance of oxygen. Generally, in humans they live in the gastrointestinal tract, but are also found in other places outside the body, such as, in soil and water, in foods, and in animals. Some anaerobes are beneficial to humans, although many others can cause sicknesses, such as appendicitis, diverticulitis, and gingivitis. Qualities that are included in anaerobic bacterial infection are nasty smelling pus, abscesses, and the endangering of tissue. An example of an anaerobic bacteria is bacteroides genus, which is both beneficial and harmful to humans. Secondly, is anthrax, a disease caused by bacillus anthracis (bacterium). In most cases, this is a deadly disease that puts both humans and animals lives at stake. With the large amount of financial, and motivational support scientists have present day, researchers have developed an effective vaccine to prevent this disease. Although not all cases of this have been cured and or prevented by this vaccination, many cases have profoundly responded and ended in a much more positive outcome. Coccus is a bacteria that is shaped like an oval or sphere. Cocci are the plural for cocccus, while a single coccus bacterium is referred to as a monococcus. While some can benefit from life, by maintain balance within

Bacteria, which is the plural form of bacterium, is a large group of unicellular organisms. They are prokaryotes, which means that they do not have a nucleus and other various organelles. They are the smallest living cells, and these cells are both beneficial and detrimental to the human body. However, antiseptics are applied to the epidermis (usually hands) of organisms to decrease the chance of receiving an illness. Antiseptics, such as rubbing alcohol or hand sanitizers destroy bacteria by changing the pH level of the environment that the bacteria remain in. The change of the pH level makes it difficult for the bacteria to survive in the environment it invades. In this lab, we are trying to figure out the effectiveness of these particular antiseptics: Bath and Body Works “Ocean” (A), Germ X (B), Bath and Body Works “I Love Geeks” (C) and no antiseptic (D). The effectiveness of this sanitizers is based on whether they can inhibit bacteria or not.

As for the keyboard (Figure 3), the colonies were spread out, unevenly, and the mouse pad’s bacteria colony (Figure 2) was barely visible. What was surprising was the fact that the most touched region was not the area with the most bacteria. It was actually one of the least touched regions, because many people restrain from touching the screen, since it may cause the it to have finger imprints on it. Not only this, but the difference in colony sizes sparked the idea that they may be different types of bacteria on each region of the laptop. The data suggested that the bacteria from the keyboard and mousepad are the same, most likely someone's hands. Through this journey, the experiments learned the importance of cleaning not only their hands, but their laptops also because it may even carry various amounts and types of bacteria.

This assignment will encompass how the results of the personal hygiene and susceptibility microbiology experiments provide a framework for the basis of the NICE Clinical Guidance (CG139) on Infection and how crucial the reasons for the hand wash protocol and hygiene is in all healthcare settings.