The Use Of Biogenesis Theory And Its Effect On Microbial Growth

Dr. Charbonnet believed that diseases appeared within humans similar to how certain forms come from inanimate objects like fleas from dust; this is called spontenous generation. Charbonnet argued that the microbes are the result of disease meaning that the microbes are not the reason for causing an illness but come after someone contracts a disease. Unlike Pasteur’s idea, he said that microorganisms are

-Labs facilitated the establishment of the germ theory; because it was the place where full time scientists and researchers, studied disease, germs, bacteria, and viruses.

There were two tubes used in this process: the tube that contained the primary culture and the tube that contained the nutrient agar where the unknown bacteria would grow. First, the inoculating loop was flamed. After removing the caps of both the test tubes, they were flamed to prevent contamination of the unknown bacteria. The inoculating loop was cooled for a few seconds and was then placed into the test tube containing the bacteria. The inoculating loop with the bacteria was placed into the nutrient agar test tube for cultivation. Before the test tubes were capped, they were flamed once again. Also, isolation of the unknown bacteria had to completed. Nutrient agar was placed in the petri dish, and was left to gel for a few minutes. After the agar gelled, the inoculating loop was used to acquire bacteria and streak the unknown onto the plate for

Our world has changed dramatically since the day Antoine van Leeuwenhoek discovered microorganisms in 1676 using a simple microscope. In early days, scientists first thought life arose from inanimate materials. This theory, known as abiogenesis or spontaneous generation, was disproved later on by scientists including Lazarro Spallanzani and Louis Pasteur. The experiments conducted by these scientists showed that living things could only arise from preexisting life, or biogenesis. All life begins with a living cell, composing of five required components. These components are DNA, RNA, cell membrane, ribosome, and cytoplasm. As more investigations on bacteria were conducted, scientists were able to acquire a deeper knowledge of the microbiology and pathology of animals, plants, and humans.

    Secondly, I present to you the “Law” of Biogenesis, which is actually not a law at all. This “Law” states that matter can arise spontaneously from other matter. Some would say that Biogenesis is an existing fact, because of the Miller Urey Experiment. This Experiment, though trying to prove that Biogenesis exists, actually proves exactly the opposite. Miller tries to convey life as a spontaneous event, which formed as the effect of an accidental chemical reaction in the Earth’s atmosphere(Answers). The scientists who conducted this experiment did not use any oxygen to support their replicated atmosphere. Instead they used methane ammonia, even though our earth is supported by oxygen. Although the Miller Urey did

By the time Pasteur was doing his researches, there was an accepted theory based on spontaneous generation. This spontaneous generation would happen without the hand of God, or else, here was exposed the enigma of life’s origin. He relied on this work and moved on to keep on searching for more evidences.

development of the germ theory of disease- with this historical brought improvement to science and technology in discovery

The purpose of this lab experiment was to measure the population growth of Escherichia coli, or E. coli, a type of bacteria that can be found in the human body, specifically in the large intestine. It works to aid in digestion as well as the absorption of essential nutrients. While E. coli is extremely beneficial to the human body and most populations are safe for humans, some cause disease if they end up in food that is eaten (Trzepacz et al.) Sickness can occur when waste reaches a water supply, eating meat that is not properly cooked, or drinking milk that is not pasteurized properly. When working with this form of bacteria, it is extremely important to keep it contained for safe measure (Belk et al.) Additionally, this bacterium has also been cited as a cause of infections involving the bloodstream that have life threatening consequences. It is increasingly troubling because antibiotics are now becoming resistant (Collington, 2009.) E. coli is like all other bacteria in the way it carries out reproduction, in that it produces asexually through the process of binary fission.

All it takes is one single cell to start a pure bacterial colony. This lab allows researchers to observe isolated highly concentrated samples of bacteria, to view their traits and to isolate pure colony samples for use in future experiments. Unfortunately successful isolation was not achieved in this lab. Possible sources of error could be that the streaking was too close together resulting in an unsuccessful isolation, the loop was not flamed adequately which permitted carryover of bacteria from one sector to the next or forgetting to let the loop cool before touching the agar. To expand this lab, researchers could collect a single colony from an agar plate and grow it inside a nutrient tube to observe the oxygen nutrient requirements for that specific sample of

While this experiment was done very carefully, there were a few errors that were made during the experiment. For instance, while a group member was putting the transformation fluid into the microcentrifuge tubes, they accidentally tried to obtain the fluid without a sterile tip and this could have possibly had the micropipette obtain the wrong amount of fluids afterwards. Also, when retrieving the E. coli colony, a group member scooped up an entire colony instead of half of it, so there were two different bacterium colonies used in this

Purpose: To learn and employ aseptic technique and basic forms of culture media as well as become familiar with the basic requirements of microbial growth and the methods used to control microbial growth.

An organism of unknown origin was selected by the course instructor in an effort to properly identify the organism with the utilization of a series of biochemical tests. This exercise was of extreme importance during the termination of the course because it was essential that each student be able to apply the practical knowledge learned through class lectures, and lab practicals in an effort to individualize such experience so that each student could properly handle a bacterial culture and identify its origin. In essence, the task of identifying the selected organism via various test is essential in order to determine key features of such organism. The test results ultimately disclose how the organism functions, and this information is useful for proper identification and handling of such culture.

Another purpose of this experiment is to stress the importance of knowing the identity of a microorganism. Knowing the species of microorganism present in a sample provides a

Bacterial transformation plays a huge role in this lab. Bacterial transformation is the process by which bacterial cells take up naked DNA molecules. If the foreign DNA has an origin of replication recognized by the host cell DNA polymerase, the bacteria will replicate the foreign DNA along with their own DNA. The field of study that uses bacterial transformation as their driving force for research is biotechnology. The lab that was carried out in class is just a very small example of biotechnology. Biotechnology according to www.bio.org is just simply technology based on biology that harnesses cellular and bimolecular processes to develop technologies and products that help improve our lives and the health of our planet. This field of study is used all over the world to provide for solutions for many problems that previously were not able to be solved. Some examples of areas where this technology might be used, range from the medical field, all the way to the other side of the spectrum in the agriculture business. Many patients are given the opportunity to be treated with different kinds of medicines that would not be possible without biotechnology. An example of this would be human insulin. Some people are not able to make their own insulin, this disease is called diabetes. The human insulin gene is injected into these bacteria and as a result these bacteria make human insulin which is then harvested and sold for the people that can’t make

JAK2/STAT signaling pathway was one of most significantly differentially expressed gene sets in BC compared with CP progenitors. This signaling pathway increases the ADAR1 expression in progenitor LSCs and promotes ADAR1-mediated A-to-I editing. Furthermore, ADAR1 impairs biogenesis of tumour suppressive miRNAs, thereby contributing to cancer progression. Since the let-7 miRNA family is also related to self renewal of LSC, it led the authors to investigate the correlation between JAK2/STAT pathway and LIN28B/let-7-axis, they hypothesized that the LIN28B/let-7self-renewal axis may also be disrupted by increased JAK2 signaling. This hypothesis was validated by a lentiviral transduction of BCR-ABL1 in normal progenitor, it shows an increase