The Use Of Crispr / Cas9 Technologies

CRISPR is a new gene-modifying tool that has the potential to treat numerous medical conditions by editing genes that are responsible for certain diseases. This technology is based on the ability of bacteria to destroy the DNA of invading viruses. Studies have suggested that this new technology can be applied to human cells, although the idea of chopping up regions of the human genome can be unethical and could even be harmful. In order for the treatment to be administered to a patient, a small piece of RNA and an enzyme that makes a cut in the DNA are delivered to the cells. A biotechnology company, known as Editas Medicine, located in Cambridge, MA, is already designing treatments for conditions of the blood and the eye using CRISPR. For

During January of 2013, scientists based at the Broad Institute of MIT and Harvard were able to demonstrate fixed change in human and animal cells using CRISPR-Cas9. This marked a turning point in genetic advancement—though CRISPR was far from mastered, its potential was beginning to show.

CRISPR has been garnishing a lot of media attention recently and it is not just popular among the scientific community but also the general public. Several online news outlets and scientific journals have been talking about the significance CRISPR-Cas could have for the field of genetics and science as a whole. I even came across a Youtube video from The Verge, a tech channel that normally does reviews on new smartphones and laptops talking about CRISPR [15]. So why is CRISPR gaining so much attention both from the scientific community and the general public? The answer lies in the potential this technology possesses.

The current applications of CRISPR have improved thoroughly since the first days of transgenic creations, and now with the help of the previously mentioned transgenic organisms, CRISPR has been created. CRISPR is currently being used in a variety of ways, whether it be through adaptation to severe weather, or even through creating medication to cure previously incurable diseases. There are no lengths

CRISPR stands for Clustered Regularly Interspaced Short Palindromic Repeat, referring to the repeating DNA sequences found in the genomes of microorganisms. CRISPR technology allows scientists to make precise changes in genes by splicing and replacing these DNA sequences with new ones. Through these changes, the biology of the cell is altered and possibly affects the health of an organism. The possibilities are endless as this offers opportunities in curing deadly diseases, modifying genes, and changing humanity as we know it. Although bioengineering has been around since the 1960s, CRISPR is significant because of the comparative low costs and the ease of the procedure to

CRISPR Cas-9 is a system that changes genes and shows a further promise for treatment for Duchenne Muscular Dystrophy (DMD). Doing this will hopefully avoid ethical dilemmas. DMD effects nearly 1 in 5,600-7,700 people between the ages of 5 and 24 for males in the U. S. CRISPR stands for “Clustered Regularly Interspaced Short Palindromic Repeats”. These are DNA segments found in microorganisms that repeat itself in open spaces. CRISPR attacks any virus that comes into your body and cuts the strand of virus on the DNA out. Researchers have tried to cure DMD in mice by getting therapy through the back of the eye, one of the ways CRISPR therapy works, which seems to work best.

The author gives a brief history of past genome editing but thoroughly explains the history and mechanism of the CRISPR technology. She elaborates on how the technology has already been used to cure diseases and speculates on its future uses and regulation.

Every few years, advancements in technology alter the way scientists do their work. Recently CRISPR-Cas9, a RNA useful for working organisms in the animal kingdom has proven itself beneficial on a gene-editing platform. After performing many abortive attempts to manipulate gene function, including homologous recombination and RNA interference, scientists have finally had a breakthrough with CRISPR-Cas9.

CRISPR Cas9 has many advantages, but it has generated a lot of excitement in the scientific community because it is faster, cheaper, more accurate, and more efficient than other existing genome editing methods. CRISPR reduces the time required to modify targeted genes and has improved bioinformatics tools to identify the best sequences to design guide RNAs. CRISPR Cas9 in embryos works extremely well for the generation

CRISPR/Cas9 DNA editing system is a prokaryotic immune system that becomes resistant to foreign genetics such as plasmids. CRISPR stands for Clustered Regularly-Interspaced Short Palindromic Repeats, which are prokaryotic DNA strands that have a short repetition base sequence. Cas9 is a nuclease enzyme that is used to cut into DNA strands that are associated with CRISPR type II. CRISPR/Cas9 system has several advantages when using this technology in the biological and biomedical sciences. It also has a few disadvantages that cause concerns when using this system in the medicine field. However, before all of this can be discussed, a depth of what CRISPR/Cas9 is and where it comes from and why is it important to the biology field.

CRISPR is versatile in that any target sequence can be modified by simply altering the gRNA sequence. In addition, multiple genes can be edited at the same time with great specificity (Cong 88-89). The convenience and accessibility of CRISPR resourced have also allowed thousands of laboratories worldwide to study CRISPR in different ways, which has broadened the horizons of its biomedical and clinical implications (Collins et al 259). Overall, the ease and simplicity of CRISPR technology has allowed for a rapid increase in the understanding of genome editing, which will allow CRISPR to revolutionize how certain conditions will be treated.

A research team at the University of Texas Southwestern medical centre have stated that by using the CRISPR-cas9 system they can target the disease within mice, and reattach fibres, therefore curing the disease. The mice after the CRISPR-Cas9 enzyme was added, were able to generate more force, grip stronger and even resist eccentric contraction and reduced blood levels of Creatine Kinase. However out of all these points the most

Diseases are something that we all fear to have. Many life changing diseases exist that may one day be in you. CRISPR, as stated previously, has potential. Curing or at least helping to eliminate diseases can be possible with CRISPR. By editing human embryos or the cause of the disease, major diseases may have the potential to be wiped off the Earth in the future, a major positive.

There are around 6,000 known genetic disorders (Genetic Disease Foundation). The symptoms of these disorders range from memory loss to blindness, physical abnormalities and more. A process known as gene editing was created in an attempt to do away with genetic disorders. Gene editing was named “Science Magazine 's Breakthrough of the Year 2015” due to its ease and high accessibility (ScienceDirect). It works by using CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) and Cas9 to make changes in the genes of cells. Francisco Mojica discovered CRISPR in 1993 at the University of Alicante in Spain (Broad Institute). It works by adding, changing or even removing DNA bases (Your Genome). Alexander Bolotin discovered Cas9 in 2005 at the French National Institute for Agricultural Research (Broad Institute). Cas9 is an enzyme that “acts as a pair of ‘molecular scissors’ that can cut two strands of DNA at a specific location in the genome so that bits of DNA can then be added or removed” (YourGenome). “The CRISPR-Cas9 system has been used to correct genetic mutations and for replacing entire genes, opening up a world of possibilities for the treatment of genetic diseases” (ScienceDirect). This makes CRISPR-Cas9 a new and revolutionary technology with a wide range of potential applications, and almost everybody will be affected by gene editing in one way or another. The scientists currently working on making human gene editing a reality, is only one group of

The CRISPR technology originates from the bacterial research, and about one decade’s efforts have been devoted to study its underlying molecular mechanism. The developmental history of the CRISPR-Cas9 system has revealed that how basic biological research will drive the progress of biotechnologies and therapeutic methods.