The X 3c : Anaphase

1455 WordsApr 3, 20176 Pages
Fig 3C: Anaphase, 3. Switch to high power and center your slide in the apical meristem region, so that you have a field of view in which there is a wide selection of cells in various stages of mitosis (including interphase). Be sure to adjust your light for optimum viewing. Refer to Figure 3A, 3B, 3C: Stages of mitosis in onion root tip cells for guidance. Find a representative cell for each stage of mitosis on the onion slide and draw a clear diagram of the different phases you observed in the accompanying space. Be sure to draw only what you see, but include all details that are visible. Your drawings will not necessarily look exactly like the ones in Figure 3A, 3B, 3C. Interphase Prophase Metaphase Anaphase Telophase Cytokinesis 4.…show more content…
Then multiply this fraction by the total time (720 minutes) needed to complete one mitotic division. In other words, the time for a specific phase is equal to: Number of cells in a specific phase ----------------------------------------------- X 720 minutes = Time for specific phase Total number of cells counted 9. Using your data, calculate the time required for the completion of each stage of mitosis in onion root tip cells. Be sure to use the totals for all three count areas. Record these calculated results in the appropriate column of Data Table 1. 10. Now, calculate the percentage of the cell cycle spent in each phase and record it in Data Table 1. 11. Prepare a bar graph to illustrate your results. Your independent variable is the stages of mitosis and your dependent variable is the “time (minutes) to complete stage”. DATA TABLE 1. COUNT AND TIMING OF CELLS IN VARIOUS STAGES OF MITOSIS Stages of Mitosis # cells Area 1 # cells Area 2 # cells Area 3 TOTAL # cells TIME in Minutes % Cell Cycle Interphase Prophase Metaphase Anaphase Telophase TOTALS 720 100% PART 3. MITOTIC DIVISION IN CANCER CELLS A key characteristic of cancer cells is that they are no longer constrained by the standard cell cycle controls that normally coordinate cell division activity. Consequently the timing of mitosis in cancer cells is altered. You may

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