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Thin Layer Chromatography Lab Report

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Sugar content in organic products versus natural products tested with thin layer chromatography

Carbohydrates are one of the most important components for food sources. Carbon based molecules are also called organic compounds. Large organic molecules have elaborate shapes. The chain of carbon atoms is called a carbon skeleton. Other monosaccharides carbon skeletons may have 3 to 7 carbons. 5 carbon sugars are called pentoses and six carbon sugars are called hexoses, which are the most common. The formula for a Monosaccharide Carbohydrate is CH2O. Carbohydrates refer to a class of molecules ranging from small sugar molecules to large polysaccharides (long chain of monosaccharides).(Reece 2012) These sugars behave differently and have different …show more content…

By testing with a method called thin layer chromatography (TLC) you can compare the compounds in the sugars. TLC is a quick, inexpensive procedure that tells you how many components are in a mixture. TLC is also used to support the identity of a compound in a mixture. A TLC plate is a sheet of glass, metal, or plastic which is coated with usually silica or alumina. A small amount of the mixture that will be analyzed is spotted near the bottom of the plate. The plate is then placed in a shallow pool of a solvent into the developing chamber so that only the very bottom of the plate is in liquid. This liquid slowly rises up the TLC plate by capillary action. Capillary action is the tendency of a liquid to rise in narrow tubes or to be drawn into small openings. (Capillary Action) As the solvent moves past the spot that was applied, an equilibrium is established for each component of the mixture between the molecules of that component which are adsorbed on the solid and the molecules which are in solution. When the solvent has reached the top of the plate, the plate is removed from the developing chamber, dried, and the separated components of the mixture are visualized. If the compounds are colored, visualization is straightforward. Usually the compounds are not colored, so a UV lamp is used to visualize the plates. (The plate itself contains a fluorescent dye which glows everywhere except where an …show more content…

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