Thin Layer Chromatography Lab Report

The main goal of this lab was to determine which of the acetaminophen and acetylsalicylic acid exists in Anacin and which exists in Tylenol. In order to achieve this goal, Thin Layer Chromatography, an approach that uses the difference in the strength of intermolecular forces and polarity in molecules to separate mixtures, was used

Separation and Purification of the Components of an Analgesic Tablet. Cora Bruno, Lab Section E. Aspirin, Caffeine and Acetaminophen were separated from four analgesic tablets of Excedrin using extraction techniques. 5% wt/vol NaHCO3, 4M HCL, ethyl acetate and deionized water were used to separate the three active components. MgSO4 was used to dry each extraction. Aspirin was isolated using a hot water bath and weighed to determine the percent theoretical recovery and the actual percent recovery of aspirin. After separation, Aspirin (ASA), Caffeine (CAF), and Acetaminophen (ACE) were purified and identified using Thin Layer Chromatography (TLC). Standards and purified ASA, CAF, and ACE were spotted on the silica gel (stationary phase) of the

There are various techniques to separate a mixture of compounds from each other. One of the commonly used way to isolate compounds from a mixture of two compounds is called extraction. This method of extracting two compounds from each other relies on the different solubility of the compounds in two different solvents.

In this study, the use of an HPLC instrument with a UV/Vis detector to determine concentration of caffeine and benzoic acid in an unknown solution mixture will be evaluated by its ability to calculate these concentrations in Mountain Dew.

Substance A and B were weighed; Substance A weighed 0.502 g and substance B weighed 0.503 g. Both substances were put into two different test tube with approximately 8 ml of DI water into the test tub. Substance A and B were stirred and B dissolved while A did not. This shows that B is soluble in water compared to A. Thus, shows that B is soluble in water than A. The reason why B is soluble in water is because it has a higher dipole moment than A. With a higher dipole moment, it shows that it is soluble in water since it is polar and the bonds were easily broken.

The objective of this lab was to use qualitative and quantitative measurements and observations to properly identify, and then confirm the identity of an unknown compound. After identification, the compound would have to be synthesized, in our case, through an acid-base reaction, followed by confirming its identity with the original unknown compound.

In this experiment, several analgesics were analyzed by Thin Layer Chromatography (TLC) and the composition of an unknown tablet was identified. We define chromatography as the separation of two or more compounds or ions by their molecular interactions by either a moving or a stationary phase.1 There are different types of chromatography: Thin Layer Chromatography (TLC), Gas Liquid Chromatography (GC), and Column Chromatography (CC). All of which there two phases:

| Analysis of urine by physical, chemical, microscopical means to test for the presence of disease drug.

Pharmaceuticals, although known under many different names, have been an important part of civilization since the Ancient times. They are extremely important in the everyday life of many Canadians who deal with illnesses, diseases, and pain. Chemistry is an integral part of medicines. Were it not for medicines and the pharmaceutical industry, the world would be a very different, very unhealthy place. Medicines are easily taken and forgotten today, but that was not always so.

The color of food is an integral part of our culture and enjoyment of life. Who would deny the mouth-watering appeal of a deep-pink strawberry ice cream on a hot summer's day or a golden Thanksgiving turkey garnished with fresh green parsley?

To prevent the escape of organic vapors, the reaction mixture is cooled with an ice bath before removing the condenser. The next technique used in this experiment was simple distillation. This is a physical separation of the components of the mixture. This technique is accomplished once the drip rate of the product into the collection vessel diminishes considerably. After the reflux and distillation is complete 13C NMR and IR is used to identify the product or products for each reaction. 13C NMR is used to observe the carbon skeleton of an organic molecule. Analysis of this spectrum allows certain stretches to be observed. An IR spectrum has energy measured as frequency recorded on a horizontal axis and intensity of the absorption on the vertical axis. Analysis of the IR allows us to differentiate between certain characteristics and functional groups in organic chemistry.

The lab we did utilizes the varying acidity, polarity, and solubility of aspirin, acetaminophen, and caffeine to teach undergraduate organic chemistry students a variety of laboratory techniques. The purpose of this lab was to use the properties of the active components in Excedrin to learn and apply the techniques of acid-base extraction and Thin Layer Chromatography. Utilizing these techniques, we were able to separate the three components of Excedrin, confirm their identities, and check the purity of our products. The theory behind the acid-base extraction we conducted is to use the acidity level of each of the components of Excedrin to separate them without using the common separation method of temperature changes. Acid-base extraction is used to isolate compounds by using ionic salts like K2HPO4 and KOH to make the compound water soluble then adding an acid like HCl to protonate the compound and make our desired product precipitate out since it returns the component back to neutral.

Chromatography is a separation technique in which the mixture to be separated is dissolved in a solvent and the resulting solution, often called the mobile phase, is then passed through or over another material, the stationary phase. The separation of the original mixture depends on how strongly each component is attracted to the stationary phase. Substances that are attracted strongly to the stationary phase will be retarded and not move alone with the mobile phase. Weakly attracted substances will move more rapidly with the mobile phase.

The molecular structure of a substance can help to predict the toxicity of it. By comparing the substance 's structure to that of more than 12,000

By Using specific methods of compounds detection, we can match an unknown compound with a known compound because similar compounds will display similar characteristics. In this experiment, identifications of the unknown ketone was accomplished using thin layer chromatography, melting point, and NMR spectrometry. Thin layer chromatography is very quick but sensitive way of determining the components of a mixture or a specific compound from a list of knowns. This method is the one which was utilized in the