Thin Layer Chromatography Lab Report

1791 WordsOct 15, 20128 Pages
OBJECTIVE The purpose of this experiment is to analyze mixtures of compounds prior to, during and after a separation scheme. This experiment also allows monitoring reactions of organic molecules, and determines the identity of a mixture of compounds. STRUCTURES AND PHYSICAL PROPERTIES OF REACTANTS [1] SOLVENTS | a. Hexane1Molecular Molarity: 86.18 g/molBoiling Point: 69 ºCMelting Point: -95ºCDensity: 0.659 g/mL at 25ºCWater Solubility: Insoluble in waterColor/Texture: Colorless/LiquidHazardous Information: May cause irritation to the eyes, skin and respiratory system. May cause drowsiness and/or dizziness. Suspected of damaging fertility or unborn child. May cause damage to organs. Toxic to aquatic life. Highly flammable liquid and…show more content…
The distance traveled by Benzophenone was 2.9cm, which gives it an Rf value of 0.67. The third spot contained Biphenyl, which traveled a distance of 3.6cm, which gives it an Rf value of 0.84. Unknown B had two spots: the first spot had a traveled distance of about 2.3cm which gives it an Rf value of about 0.53 and the second had a traveled distance of about 2.9cm, which gives it an Rf value of about 0.67. Judging from the Rf values of unknown B, the two compounds present are Benzhydrol and Benzophenone. DISCUSSION Separation of compounds is a result of competition by the stationary phase (adsorbent) and the mobile-phase (developing solvent). Generally, a non-polar developing solvent should be used for non-polar compounds and a polar developing solvent for polar compounds. Selecting a solvent is a trial-and-error process. Solvents that are not polar enough does not cause the original spot to move far enough, as well as, solvents that are very polar causes all spotted material to move along the solvent front. As a result, both give no to
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