Thin Layer Chromatography Of Amino Acid

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Thin- Layer Chromatography (TLC) of Amino Acids
Thin layer chromatography (TLC) separates compounds, through migration, from a mixed solution with the assistance of a solvent and an absorbent strip of cellulose. The purpose of this lab is to allow students to determine an unknown amino acid by comparing results to six known amino acids (slowest to fastest: lysine, glycine, alanine, tyrosine, phenylalanine, and leucine) and properly separate a combined solution of amino acids using the TLC method. But to fully understand the lab, two terms must be understood: amino acid and protein. Amino acids are building blocks of proteins linked by peptide bonds that contain both carboxyl and amino functional groups.
The six amino acids used in this experiment:
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Rf values for known and unknown amino acids. Lanes 2 and 4 were used to identify the unknown (Unknown T) amino acid with the slight assistance of Lane 1.
Based on my results from Lanes 2 and 4, I predict Unknown T to contain both lysine and glycine based on the slow reaction/movement the unknown experienced once the solvent was introduced and the shortest distance it underwent. My prediction was supported with the development of Lane 2. As I analyzed Lane 2, it became obvious that the six Rf values represented the six known amino acids; the lowest Rf value was lysine and the highest Rf value was leucine. Once the Rf values for the unknown was calculated it was easy to identify the two amino acids present in the unknown sample due to corresponding values in both lanes. Although one value (Rf = 0.515) in Lane 4 did not completely match with the second value (representing glycine) in Lane 2, I can conclude that the Rf value in Lane 4 was still glycine because the values are similar and close in range, and Lane 1 helps support my theory as
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