Thin Layer Chromotography Discussion

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Thin-Layer Chromotography Discussion and Conclusion
Easton Montgomery

In thin-layer chromatography a liquid is pumped across a bed of particles. The liquid that is pumped across is called the mobile phase and the particles are the stationary phase. A mixture of the molecules that will be separated is put into the mobile phase. Thin-layer chromatography tells you/helps you determine the number of compounds in a mixture, the purity of a compound, and the identity of compounds if you have examples to pull information from. Thin-layer chromatography is used to separate nonvolatile mixtures. The dye that was the most polar was the color red and pink which was Rhodamine B and the least polar was the light pink color which was Sudan IV. Our first TLC plate had five
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You can use thin-layer chromatography for quantitative analysis to some extent. If you use solutions of known concentrations and compare the unknown to the standards, then you can gain some quantitative information. You can also calculate Rf values taking the distance traveled divided by the total distance of the eluent. After we chopped up our spinach leaf we used hexane and ethanol to separate out compounds in our leaf. After adding water, we removed the top layer and spotted a TLC plate to separate out our compounds. We also used a mixture of hexane/ethyl acetate (1:1) and hexane/ethyl acetate (20:1) to use as our developing solution. The (1:1) solution gave use six compounds separated out on a TLC plate with xanthophyll being the most polar and carotene being the least polar. On the other hand, the (20:1) solution gave us one compound that moved through our TLC plate. Looking at the plate it seems like it is the carotene that moved while everything else remained near the polar side of the plate. The most polar molecule was Rhodamine B (bright pink-red) then Fast Green (blue) then we had a clear compound show up, then Bismark Brown (yellow-orange) and the least polar compound was Sudan IV
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