Three hundred and sixty feces samples were collected from children and infants who have diarrhea
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Three hundred and sixty feces samples were collected from children and infants who have diarrhea and other symptoms that associate with Acute Flaccin Paralysis (AFP) from middle and southern provinces of Iraq. They were equal or less than four years old. Preliminary culture shows total samples that gave positive to poliovirus were 19 samples and number of samples that have poliovirus type I were (6), type II (3) and type III (6), number of samples that have mix types of poliovirus were (4). Confirmed results show the numbers of samples that contain type I, type II and type III were 9,3and 10 in respectively and all viruses were sabin type only (There is no wild types of poliovirus were isolated).
Poliovirus belong to…show more content… But the last associated with many problems, First the live oral polio vaccine is known to be able to cause poliomyelitis in rare cases in both vaccines and their contacts (3). Second some hypogammaglobulinemic patients are known to excrete virus for long periods of time, giving opportunities for the virus to evolve to transmissible and virulent forms (9, 10). The aim of this study is showing of presence of wild and sabin (vaccine of poliovirus) in stools of many cases of children in Iraq and outbreak them.
MATERIALS AND METHODS:
Samples: Two – four grams of faces of 360 samples were collected from children and infants who have diarrhea and other symptoms that associate with Acute Flaccin Paralysis (AFP). All samples were collected from Al-Sadar learning hospitals in middle and south provinces of Iraq. The period of collection (1-1-2006 to 1-7-2006) All samples were collected from patients have less than four years old.
Preparation of fecal samples for virus isolation:
10 ml of Phosphate Buffer saline (PBS) PH 7.2, 1 gm of glass beads and 1 ml of chloroform were added to each tube. The tubes were closed and shaken vigorously for 20 minutes using a mechanical shaker. All tubes were spun for 20 minutes at 1500 g in a refrigerated centrifuge. Fecal suspensions were stored at -20 ○C till using .
Fecal suspensions were inoculated into tissue culture tubes of RD cells line (cells line derived from human rhabdomyosarcoma) and into