Tlc Plate Chromatography

According to the results, the columns of caffeine in figure 1, of this experiment the hypothesis for caffeine is partially accepted. There is an increasing trend in the change of pulsation rates with increasing

TLC Analysis Data Table Compound Std. Aspirin Std. Caffeine Rf values 0.38 0.65 Error ---~ 0% 29% 5%

In this study, the use of an HPLC instrument with a UV/Vis detector to determine concentration of caffeine and benzoic acid in an unknown solution mixture will be evaluated by its ability to calculate these concentrations in Mountain Dew.

← I would repeat the experiment with the caffeine solution more times, so that the results I would get will be more

5.    Prepare the caffeine solution by dissolving 10g of caffeine tablets in 100ml of water in a beaker. Label the beaker ‘caffeine’. Similarly, add 10g of coffee to 100ml of water in another beaker and label it ‘coffee’.

Which one of the statements concerning valence bond (VB) and molecular orbital (MO) bond theories is correct?

1. Which solvent or solvent mixture did you decide to use for your TLC? Why?

In this experiment, the purpose of this experiment is to isolate pure caffeine from tealeaves. Caffeine does not exist alone within the tealeaves, but other substances that are in the leaves itself accompany it. There are major and minor products that make up the constancy of the leaves and with those substances they need to be separated out of the leaves. The main component of the leaves are, cellulose and polymer of glucose, while the minor components of the leaves are caffeine, tannis, flauaniods and chlorophylls. By weight, caffeine is 5%, which is an alkaloid and contains C, H, N, O; it is very soluble in water and is the main component that will be extracted from the experiment. Although caffeine is very soluble in water it is much more soluble when it is dissolved in methylene chloride. Therefore, when completing the methylene chloride process, it will nearly purify all of the caffeine, making it pure caffeine. Make sure not to breathe in the fumes of the chemicals or spill them on yourself. Safety precautions

Components containing caffeine were composed into stock solutions. These solutions were diluted to 1: 10 substance: mobile phase. A stock solution of caffeine was diluted 1:50. A sequence of diluted caffeine solutions were prepared for use as a standard (ppm): 1, 2, 4, and 10. Solutions of acetaminophen, acetylsalicylic acid, and Goody’s Powder were developed to differentiate chromatographic peaks observed. These solutions were subjected to HPLC for examination of the observed peak area and retention time for the set of compounds. Comparison of retention time allowed for the differentiation of peaks observed. The peak area obtained was utilized to determine the relative concentration of caffeine present in Goody’s Powder based on the relationship obtained in the standard. The content of caffeine present in Goody’s Powder by percent weight was identified.

- When marking the TLC plate with a pencil, there should be no pressing the plate surface with the pencil because the silica will be removed from the plate surface.

AIM: To extract codeine and paracetamol from its tablet by solvent extraction and tentatively identify in comparison to standards using Thin Layer Chromatography.

In this experiment, paper chromatography was used to determine what pigments were present in spinach extract. From this experiment, we can see that four different types of pigments are present in the spinach extract used, the following are those pigments: chlorophyll a, chlorophyll b, beta carotene, and xanthophyll. The absorption and reflection of these pigments all revolve around the basis of the electromagnetic spectrum. The form of electromagnetic radiation is released as light and overall it is a type of energy that travels in waves. Going back to the spectrum itself, all the different types of electromagnetic radiation combine to form the electromagnetic spectrum, which tells us which colors can be absorbed and/or reflected. Each wave

Chromatography is a separation technique in which the mixture to be separated is dissolved in a solvent and the resulting solution, often called the mobile phase, is then passed through or over another material, the stationary phase. The separation of the original mixture depends on how strongly each component is attracted to the stationary phase. Substances that are attracted strongly to the stationary phase will be retarded and not move alone with the mobile phase. Weakly attracted substances will move more rapidly with the mobile phase.

Chromatography Investigation Chromatography is a highly regarded technique used to separate the components of a mixture. It is based on the principle that each component possesses a unique affinity for a stationary phase and a mobile phase. The components that are more inclined to enter the mobile phase will migrate further on the chromatogram and distinguish themselves from the other components. The type of solvent used in chromatography is known to directly affect the separation of the mixture. In this experiment, thin-layer and column chromatography will be utilized to separate the numerous chlorophyll and carotenoid pigments of a spinach extract.

AIM : Thin-Layer Chromatography can show many different characteristics of a mixture. It is recognized for isolation , separation ,identification, and anaylsis of the mixture’s components. The purpose of this experiment is to separate carbohydrates into its pure components such as mixtures of monosacrides by TLC. TLC is used to identify sugars in normal and pancreatic disease urine, the procedure is easy and reproducible .