Tlc Plate Chromatography

NAME: ____Amy Hua_______________________ Experiment 4a Adsorption Chromatography (TLC) Summary of Points for Experiment 4a: Item Possible Points Actual Points Pre-Lab 2 Notebook: N/A N/A Purpose/Table of Reagents 2 Corrections 2 Blank Spaces 2 Signatures 2 TLC data (4-in notebook) 8 Coherent 2 Conclusions (absent here) 1 Sub-Total = 21 multiply Sub-Total x 2= 42 Report: N/A N/A Introduction 2 Data and Calculations 8 Less Points-Missing Data N/A N/A Unknown Identity 10

Thin Layer Chromatography and Column Chromatography By Maggi Shelton Under the Supervision of Dr. Mills Chavonda The Department of Chemistry and Physics Milledgeville, GA 31061   Abstract: Thin layer chromatography and column chromatography are two different methods that allow for the separation of two miscible solvents. Through column chromatography, a mixture of nonpolar fluorine and polar fluorenone was successfully separated. Thin layer chromatography was then used in order to determine the success

Chromatography of Commercial Analgesics Using the thin layer chromatography the major components of a commercial analgesic tablet will be indentified and purified. The active ingredients of the analgesic used were characterized by comparison with chromatograms of aspirin, acetaminophen, ibuprofen, mefanemic acid, and caffeine through the use of UV chromatography then the Rf value was calculated for each solvent. INTRODUCTION In this experiment, thin-layer chromatography (TLC) will be used

208). The main objective of this experiment was to extract these pigments from spinach leaves using chromatography techniques. First, the pigment was extracted from the leaves by column chromatography. However, the column was packed with granular anhydrous sodium sulfate. This first extraction acted as E or the main extract of the pigments. Using a small sample of the extract, column chromatography was conducted again to separate the pigments that are combined in the main pigment extract.

Chromatography Different types of Chromatography Paper chromatography Paper chromatography is a method of testing the purity of compounds and helps identify the substances within it. Using the paper chromatography is a useful and simple technique as it is relatively quick and requires small quantities of material. Paper chromatography separates colours, this also happens in the thin layer chromatography. Using the paper chromatography method, substances the distribution between stationary phase and

COLUMN AND THIN LAYER CHROMATOGRAPHY Mark Paul P. Pastrana, Mariah Ericka M. Patawaran, Princess Juneire M. Peligro, Francisco Q. Pua III, Rose Anne L. Quyo and Janille P. Ragpa Group 8 2B Medical Technology Organic Chemistry Laboratory ABSTRACT The main objectives were to separate the colored components of malunggay leaves by means of column chromatography, as well as to determine the purity of the components using thin layer chromatography (TLC) and measure the Rf values of the colored

Title Page Chelsea Maksin Organic Chemistry Laboratory 1 Column and Thin Layer Chromatography: The Separation of Spinach Pigments Russ Hoburg 02/15/2012 Abstract The main goal in doing the column and thin layer chromatography was to separate spinach extract into its components based on polarity and then to analyze the components. The separation of the spinach extract was done using the column chromatography with the wet/slurry packing method. Alumina was inserted into the column to act as

Background: Derived form the greek words for colour (Chroma) and writing (graphe). Chromatography is a method is method in which different kinds of the coloured chemical mixtures are separated. In the early 1900s, Mikhail Tswett, a Russian botanist, was interested in the individual compounds presented in plants. He understood that removing ground up plants extracts with the dissimilar solvents will provide assorted coloured solutions. Tswett conducted an experiment which involved pouring a plant

By: Salien Mansi Student ID: Smansi2 [TLC Separation of Amino Acids] Objective: Separation and identification of the amino acids in a mixture by thin layer chromatography. Experiment was done to understand the effects of Thin layer Chromatography using various concentration mixtures of solvents. Thin layer chromatography [TLC]: The importance of Chromatography is for the separation of compounds in a mixture. The separation happens between the stationary and the mobiles phases. This experiment

Introduction Column Chromatography is common and useful separation technique in organic chemistry. This separation method involves the same principles as thin layer chromatography but can be applied to separate larger quantities than TLC. Column chromatography can be used on both a large and small scale. The applications of this technique are wide reaching and cross many disciplines including biology, biochemistry, microbiology, and medicine. The technique of TLC is useful in determining the type