Aim
The purpose of this experiment is to determine the percentage by mass of calcium carbonate in eggshells.
Introduction
To avoid the breakage of eggs before reaching market, the eggshells needs to be as strong as possible. The strength of eggshells is mainly determined by the percentage of calcium carbonate in it. In order to monitor the quality of eggshells, the following experiment has to be done to determine the percentage of calcium carbonate in eggshells.
In this experiment, back titration is used. First, excess acid is reacted with the calcium carbonate in eggshells:
2HCl(aq) + CaCO3(s) → CaCl2(aq) + H2O(l)+ CO2(g) Later, if we can find out the number of mole of unreacted acid, number of mole of calcium carbonate can
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5. A 25 cm3 aliquot of the diluted eggshell solution was pipetted to a clean beaker. The beaker was placed on a magnetic stirrer and a pH meter was put into the solution.
6. 12.5 cm3 of 0.9992 M sodium hydroxide solution was transferred to a 250 cm3 volumetric flask by burette and de-ionized water was added to the mark. The flask was shaken to ensure homogeneity of the solution.
7. A clean burette was filled with the diluted sodium hydroxide solution (~ 0.05M). The initial burette reading, accurate to the nearest 0.05cm3, was recorded. During trial run, about 2-3 drops of methyl orange was added as an indicator.
8. Sodium hydroxide solution was run from the burette into the flask with the magnetic stirrer being turned on. When the indicator was turned slightly yellowish, sodium hydroxide was added dropwisely until the reading of the pH meter is 7.00. The final burette reading, accurate to the nearest 0.05cm3, was recorded.
9. The burette was refilled with the diluted sodium hydroxide solution. Steps 7 and 8 were repeated 3 times without using indicator. Sodium hydroxide was added dropwisely when the burette reading was close to the final reading in trial run.
Diagram
Results Tables
Trial 1 2 3
Burette reading / cm3 Final 26.80 26.85 26.80 26.75 Initial 0.00 0.00 0.00 0.00
Volume used (titre) / cm3 26.80 26.85 26.80 26.75
Mean titre / cm3 26.80
Calculation
The number of moles of NaOH used in each titration
=
The purpose of this lab is to determine what is the concentration of the unknown solution along with what concentration would be more isotonic to the chicken egg?
The egg shell completely disappears due to the vinegar reacting with the egg; bubbles form on the surface of the vinegar and on the egg because carbon dioxide was produced from
The primary purpose of the lab was to test and observe the effects of the process of osmosis on decalcified eggs. Before the process, the initial weights of the eggs were taken and noted. Two eggs were then immersed in a deionized water solution. The same was repeated to the other two solutions of 20% and 40% sucrose. The weights of the eggs were measured after 15 minutes to note the change in mass due to osmosis and again after 60 minutes to take the final measurement. Overall, while some human error might arise in the weighing process, care was taken in handling the eggs and during the weighing process. As such, it was found that the eggs behaved as predicted. It was found that the eggs placed in the hypertonic solution lost
The purpose of this semester long experiment was to determine an unknown organic acid. An organic acid is an organic compound with acidic properties. A base reacts with acids to form salts. Titrations are used to determine the concentration of unknown substances. The purpose of the KHP experiment was to determine the molarity of NaOH. HCl titrations are mainly to check technique and used to verify the molarity of NaOH solution. The hypothesis is that this acid is C4H3OCOOH.
The purpose to this experiment was to study the effect of osmosis in de-shelled chicken eggs in different percentages of sucrose solutions. Osmosis is the process, in which, water moves across a differentially permeable membrane. The eggs were soaked in vinegar to remove the outside hard shell but still leave the egg in its membrane. By placing the six de-shelled in different sucrose solutions, we tested the rate of osmosis. The eggs were placed in the solutions for an hour and weighed in fifteen minute intervals. Then, each weight was recorded and graphed. The results showed that the egg in the water solution gained the most weigh and the only other egg that gained a little weight was the one in the 10% solution. All the other eggs in the different solutions lost weight, even the unknown solution. According to the results the egg that was in the distilled water solution gained weight because it is the hypertonic solution. All the other eggs lost weight because they were placed in hypotonic solutions with different concentrations of sucrose. The egg that was placed in the higher concentration of sucrose lost the most weight. So, the higher the concentration of sucrose, the more water the egg lost.
After the twenty minutes elapsed, the flask was cooled to room temperature and then titrated with the remaining NaOH until the colorless solution remained pink. The final volume was then recorded. While solution #1 was heating the same process was repeated with solution#2 and the second burette
An egg has a semi-permeable membrane, thus processes like osmosis could occur. Osmosis is the movement of water molecules from an area of higher water concentration to an area of a lower water concentration. Osmosis is important, especially for living organisms, as they help distribute nutrients in the body. An egg’s mass would change when it is soaked in different substances. The goal of this experiment was to investigate how an egg changes through osmosis. This experiment was done to show how substances affect the mass of an egg. To start off, an egg’s mass was measured by putting the egg in a beaker then placing it on a mass scale. The beaker was then filled with vinegar and left alone for twenty-four hours. After a day, the egg was taken
Did the egg permeate the corn syrup (hypertonic solution) or the water (hypotonic solution)? First, using the triple beam balance we measured the mass of our egg which was 65 grams. Then, we put the egg in a 200ml of vinegar to completely take the outer shell off. The next day, after rinsing the egg, using a beaker we poured 200 ml of corn syrup into the beaker. Then put our egg into the beaker and let it sit for 24 hours. After the 24 hours, the egg has shriveled and has gone down to 48 grams. On day 3, we poured 200 ML of water into the beaker then placed the egg into the water. the next day, the mass of the egg went above the original mass of the egg at 96 grams. The egg permeated the water because of osmosis and it’s density.
This is the final step of the experiment. The shell-less egg was now placed into a new cup, but very carefully, as the egg was more fragile than when it was soaked in vinegar. The egg in the new cup was then covered with water. The egg sat in the water for a 24 hour time period once again. During this 24 hour time period the eggs appearance and size were once again recorded. After the time period had completed, the egg was removed from the water and was very carefully patted dry. Since the egg was now swollen, extra care was needed when holding the egg. The swollen egg was placed on the scale on last time and its mass was recorded as M3.
One milliliter of 6.00-M phosphoric acid was placed into a 125-mL Erlenmeyer flask using a volumetric pipette. Using a slightly larger pipette, six milliliters of 3.00-M sodium hydroxide was transferred into a 50-mL beaker. Then a disposable pipette was used to slowly mix the sodium hydroxide into the phosphoric acid while the solution was swirled around. Then both the beaker and flask were rinsed with 2-mL of deionized water and set aside. A clean and dry evaporating dish was weighed with watch glass on a scale. Then the solution was poured into the dish and the watch glass was placed on top. The solution was then heated with a Bunsen burner to allow for the water to boil off to reveal a dry white solid. After the dish cooled to room temperature it was once again weighed and the new mass was recorded.
The purpose of this experiment was to observe the effects of different substances to an egg. When the egg’s mass increased by 11.58 grams after it was left submerged in vinegar, one could say that the vinegar is hypotonic to the egg since it was absorbed, thus increasing the mass. A solution is hypotonic when water concentration is higher outside the cell. The egg was somewhat depleted after it was left with corn syrup which could say that the corn syrup was hypertonic to the egg because it caused water to flow out from the egg thus decreasing the egg’s mass. A hypertonic solution has a higher water concentration inside the cell than outside. The colored water was hypotonic to the egg as well since it doubled the mass of the egg when the water
In conclusion, the data that was collected helped tell us that the solutions greatly affected the cell of the egg because it caused the egg’s mass to increase than decrease. Also saw how the solutions played a role in the phospholipid bilayer and saw how it altered the shape of the egg. However, it does not support my hypothesis on the affect the egg would undergo onced placed in sugar and water but sugar was not used in this experiment, but water was used and it didn’t affect the shape of the egg, all it did was cause the egg to be in a isotonic state. According to Khan Academy (2017), states that a cell is isotonic “When the extracellular fluid has the same osmolarity as the cell, there will be no net movement in or out of the cell”. Which
The purpose of this lab is to determine how the egg changes when it was in different substances which were distilled water and corn syrup. The reason the eggs change in size is because of osmosis. Osmosis is a form of facilitated diffusion when water moves through channels, water is moving from high to low. There are three types of solutions, isotonic, hypertonic and hypotonic. When the egg is placed in an isotonic solution the egg will stay the same size. Isotonic is when the concentration of water is the same outside and inside the cell and water moves equally in both directions. When an egg is placed in hypertonic solution the egg will grow because hypertonic is when the concentration of water is higher outside the cell than inside. When an egg is placed in a hypertonic solution the egg will shrink because hypertonic is when the concentration of water is higher outside the cell than inside the cell.
Experiment to investigate the amount of sodium hydroxide needed to neutralize the solution of vinegar
In this experiment we will be testing the rate of reaction of pepsin by measuring the rate at which an egg white solution is broken down